Oxidation of ethane by an Acremonium species

Davies, Julie; Wellman, A. M.; Zajic, J. E.

doi:10.1128/aem.32.1.14-20.1976

Cited by 8 publications

(2 citation statements)

References 17 publications

(17 reference statements)

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“…These investigations suggest a biochemical relatedness between fungal and mammalian liver micrgsomes towards the monooxygenation of aromatic substrates. The hydroxylation of alkanes, fatty acids, and drugs (by fungi) has also been reported (12,14,17).…”

Section: Discussionmentioning

confidence: 94%

Metabolism of naphthalene by Cunninghamella elegans

Cerniglia¹,

Gibson²

1977

Appl Environ Microbiol

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Cunninghamella elegans grown on Sabouraud dextrose broth in the presence of naphthalene produced six metabolites. Each product was isolated and identified by conventional chemical techniques. The major metabolites were 1naphthol (67.9%) and 4-hydroxy-1-tetralone (16.7%). Minor products isolated were 1,4-naphthoquinone (2.8%), 1,2-naphthoquinone (0.2%), 2-naphthol (6.3%), and trans-1,2-dihydroxy-1,2-dihydronaphthalene (5.3%). C. elegans oxidized both 1-naphthol and 1,4-naphthoquinone to 4-hydroxy-1-tetralone. The results suggest that C. elegans oxidizes naphthalene by a sequence of reactions similar to those reported for the mammalian metabolism of this hydrocarbon.

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Section: Discussionmentioning

confidence: 94%

Metabolism of naphthalene by Cunninghamella elegans

Cerniglia¹,

Gibson²

1977

Appl Environ Microbiol

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“…Although many eukaryotes are known to utilize long-chain hydrocarbons as carbon and energy sources, Graphium sp. strain ATCC 58400 is one of the few eukaryotes known to grow on gaseous n-alkanes (10)(11)(12)21). Substrate utilization studies with this species and other physiologically related fungi suggest a conventional n-alkane oxidation pathway that involves alcohol, aldehyde, and fatty acid intermediates (12,25,46).…”

mentioning

confidence: 99%

Metabolism of Diethyl Ether and Cometabolism of Methyl tert-Butyl Ether by a Filamentous Fungus, a Graphium sp

Hardison¹,

Curry²,

Ciuffetti³

et al. 1997

Appl Environ Microbiol

133

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In this study, evidence for two novel metabolic processes catalyzed by a filamentous fungus, Graphium sp. strain ATCC 58400, is presented. First, our results indicate that this Graphium sp. can utilize the widely used solvent diethyl ether (DEE) as the sole source of carbon and energy for growth. The kinetics of biomass accumulation and DEE consumption closely followed each other, and the molar growth yield on DEE was indistinguishable from that with n-butane. n-Butane-grown mycelia also immediately oxidized DEE without the extracellular accumulation of organic oxidation products. This suggests a common pathway for the oxidation of both compounds. Acetylene, ethylene, and other unsaturated gaseous hydrocarbons completely inhibited the growth of this Graphium sp. on DEE and DEE oxidation by n-butane-grown mycelia. Second, our results indicate that gaseous n-alkane-grown Graphium mycelia can cometabolically degrade the gasoline oxygenate methyl tert-butyl ether (MTBE). The degradation of MTBE was also completely inhibited by acetylene, ethylene, and other unsaturated hydrocarbons and was strongly influenced by n-butane. Two products of MTBE degradation, tert-butyl formate (TBF) and tert-butyl alcohol (TBA), were detected. The kinetics of product formation suggest that TBF production temporally precedes TBA accumulation and that TBF is hydrolyzed both biotically and abiotically to yield TBA. Extracellular accumulation of TBA accounted for only a maximum of 25% of the total MTBE consumed. Our results suggest that both DEE oxidation and MTBE oxidation are initiated by cytochrome P-450-catalyzed reactions which lead to scission of the ether bonds in these compounds. Our findings also suggest a potential role for gaseous n-alkane-oxidizing fungi in the remediation of MTBE contamination.

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Modeling the kinetics of vinyl chloride cometabolism by an ethane-grownPseudomonas sp.

Verce

Freedman

2000

Biotechnol. Bioeng.

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Oxidation of ethane by an Acremonium species

Cited by 8 publications

References 17 publications

Metabolism of naphthalene by Cunninghamella elegans

Metabolism of naphthalene by Cunninghamella elegans

Metabolism of Diethyl Ether and Cometabolism of Methyl tert-Butyl Ether by a Filamentous Fungus, a Graphium sp

Modeling the kinetics of vinyl chloride cometabolism by an ethane-grownPseudomonas sp.

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