Oxidation of Energy Substrates in Tissues of Fish: Metabolic Significance and Implications for Gene Expression and Carcinogenesis

Jia, Sichao; Li, Xinyu; He, Wenliang; Wu, Guoyao

doi:10.1007/978-3-030-74180-8_5

Cited by 15 publications

(5 citation statements)

References 79 publications

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“…This also indicated that leucine may activate AMPK and Sirt1 to activate the AMPK signaling pathway. In addition, with the increase of leucine in diet, the expression of BCKDH-related genes and proteins had a slight upward trend, and the transamination product (glutamate) [ 68 , 69 ] of leucine had increased. These might also imply that the activation of the pathway was related to the metabolism of leucine.…”

Section: Discussionmentioning

confidence: 99%

“…MRFs include myod , myf5 , myog , mrf4 , and so on [ 76 ]. The myod and myf5 mainly play a considerable role in the determination and maintenance of myoblast, while myog and mrf4 are involved in the differentiation and fusion of advanced myotubes [ 69 ]. At the moment, studies with mammals have shown that activation of AMPK can control the expression of genes related to muscle fiber growth, such as myog and mrf4 , to regulate the proliferation and development of muscle fibers [ 77 , 78 ].…”

Section: Discussionmentioning

confidence: 99%

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Dietary Leucine Supplementation Improves Muscle Fiber Growth and Development by Activating AMPK/Sirt1 Pathway in Blunt Snout Bream (Megalobrama amblycephala)

Wang

Guo

Huang

et al. 2022

Aquaculture Nutrition

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This research is aimed at evaluating the effects of leucine supplementation on muscle fibers growth and development of blunt snout bream through a feeding trial and a primary muscle cells treatment. An 8-week trial with diets containing 1.61% leucine (LL) or 2.15% leucine (HL) was conducted in blunt snout bream ( mean initial weight = 56.56 ± 0.83 g ). Results demonstrated that the specific gain rate and the condition factor of fish in the HL group were the highest. The essential amino acids content of fish fed HL diets was significantly higher than that fed LL diets. The texture (hardness, springiness, resilience, and chewiness), the small-sized fiber ratio, fibers density, and sarcomere lengths in fish all obtained the highest in the HL group. Additionally, the proteins expression related with the activation of the AMPK pathway (p-Ampk, Ampk, p-Ampk/Ampk, and Sirt1) and the expression of genes (myogenin (myog), myogenic regulatory factor 4 (mrf4) and myoblast determination protein (myod), and protein (Pax7) related to muscle fiber formation were significantly upregulated with increasing level of dietary leucine. In vitro, the muscle cells were treated with 0, 40 and 160 mg/L leucine for 24 h. The results showed that treated with 40 mg/L leucine significantly raised the protein expressions of BCKDHA, Ampk, p-Ampk, p-Ampk/Ampk, Sirt1, and Pax7 and the gene expressions of myog, mrf4, and myogenic factor 5 (myf5) in muscle cells. In summary, leucine supplementation promoted muscle fibers growth and development, which may be related to the activation of BCKDH and AMPK.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Dietary Leucine Supplementation Improves Muscle Fiber Growth and Development by Activating AMPK/Sirt1 Pathway in Blunt Snout Bream (Megalobrama amblycephala)

Wang

Guo

Huang

et al. 2022

Aquaculture Nutrition

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“…This method has not been utilized to a large extent, but should be incorporated in future studies because the plots can display a clear separation between uninfected and infected groups, as well as enzymatic activities (128). Another appealing perspective would be to look further into the effects of both pathogen and AA supplementation on epigenetics, which is defined as the heritable alterations of gene expression through covalent modifications of DNA in addition to core histones without alterations in the DNA sequence (248). This process is initiated by S-adenosylmethionine-dependent DNA and histone methylation, histone acetylation by acetyl-CoA, histone phosphorylation, and histone ubiquitination (249).…”

Section: Discussionmentioning

confidence: 99%

Immunonutrition: facilitating mucosal immune response in teleost intestine with amino acids through oxidant-antioxidant balance

Hissen,

He,

et al. 2023

Front. Immunol.

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Comparative animal models generate fundamental scientific knowledge of immune responses. However, these studies typically are conducted in mammals because of their biochemical and physiological similarity to humans. Presently, there has been an interest in using teleost fish models to study intestinal immunology, particularly intestinal mucosa immune response. Instead of targeting the pathogen itself, a preferred approach for managing fish health is through nutrient supplementation, as it is noninvasive and less labor intensive than vaccine administrations while still modulating immune properties. Amino acids (AAs) regulate metabolic processes, oxidant-antioxidant balance, and physiological requirements to improve immune response. Thus, nutritionists can develop sustainable aquafeeds through AA supplementation to promote specific immune responses, including the intestinal mucosa immune system. We propose the use of dietary supplementation with functional AAs to improve immune response by discussing teleost fish immunology within the intestine and explore how oxidative burst is used as an immune defense mechanism. We evaluate immune components and immune responses in the intestine that use oxidant-antioxidant balance through potential selection of AAs and their metabolites to improve mucosal immune capacity and gut integrity. AAs are effective modulators of teleost gut immunity through oxidant-antioxidant balance. To incorporate nutrition as an immunoregulatory means in teleost, we must obtain more tools including genomic, proteomic, nutrition, immunology, and macrobiotic and metabonomic analyses, so that future studies can provide a more holistic understanding of the mucosal immune system in fish.

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“…Remodeling of energy metabolism is an effective strategy in sh to compensate increased demand during hypoxic conditions [112] or infection [113]. The liver plays a pivotal role controlling energy reserves utilizing amino acids as primary fuel [114] with upregulated metabolism [115], [116] observed during hypoxic episodes. Prolonged hypoxia increased dominance of aerobic TCA and OXPHOS [112].…”

Section: Cellular Stress In Oxygen Minimum Zonementioning

confidence: 99%

Network-based integration of omics, physiological and environmental data in real-world Elbe estuarine Zander

Koll,

Theilen,

Hauten

et al. 2024

Preprint

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Coastal and estuarine environments are under endogenic and exogenic pressures jeopardizing survival and diversity of inhabiting biota. Information of possible synergistic effects of multiple (a)biotic stressors and holobiont interaction are largely missing in the Elbe estuary but are of importance to estimate unforeseen effects on animals’ physiology. Here, we seek to leverage host-transcriptional RNA-seq and gill mucus microbial 16S rRNA metabarcoding data coupled with physiological and abiotic measurements in a network analysis approach to deconvolute the impact of multiple stressors on the health of juvenile Sander lucioperca along one of the largest European estuaries. We find mesohaline areas characterized by gill tissue specific transcriptional responses matching osmosensing and tissue remodeling. Liver transcriptomes instead emphasized that zander from highly turbid areas were undergoing starvation which was supported by compromised body condition. Potential pathogenic bacteria, including Shewanella, Acinetobacter, Aeromonas and Chryseobacterium, dominated the gill microbiome along the freshwater transition and oxygen minimum zone. Their occurrence coincided with a strong adaptive and innate transcriptional immune response in host gill and enhanced energy demand in liver tissue supporting their potential pathogenicity. Overall, we demonstrate the information gain from integration of omics data into biomonitoring of fish and point out bacterial species with disease potential.

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Oxidation of Energy Substrates in Tissues of Fish: Metabolic Significance and Implications for Gene Expression and Carcinogenesis

Cited by 15 publications

References 79 publications

Dietary Leucine Supplementation Improves Muscle Fiber Growth and Development by Activating AMPK/Sirt1 Pathway in Blunt Snout Bream (Megalobrama amblycephala)

Dietary Leucine Supplementation Improves Muscle Fiber Growth and Development by Activating AMPK/Sirt1 Pathway in Blunt Snout Bream (Megalobrama amblycephala)

Immunonutrition: facilitating mucosal immune response in teleost intestine with amino acids through oxidant-antioxidant balance

Network-based integration of omics, physiological and environmental data in real-world Elbe estuarine Zander

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