Oxidation of Cyclic Ethers by Alkane‐Grown <i>Mycobacterium vaccae</i> JOB5

Lan, Renny Shang-Lun; Smith, Christy A.; Hyman, Michael R.

doi:10.1002/rem.21364

Cited by 23 publications

(11 citation statements)

References 41 publications

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“…may limit its effectiveness as a biomarker, multiple studies have demonstrated the ability of propanotrophs to cometabolize dioxane, supporting the use of this target as a potential biomarker for dioxane biodegradation (20,28,54).…”

Section: Discussionmentioning

confidence: 98%

Identification of Biomarker Genes To Predict Biodegradation of 1,4-Dioxane

Gedalanga

Pornwongthong

Mora³

et al. 2014

Appl Environ Microbiol

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Bacterial multicomponent monooxygenase gene targets in Pseudonocardia dioxanivorans CB1190 were evaluated for their use as biomarkers to identify the potential for 1,4-dioxane biodegradation in pure cultures and environmental samples. Our studies using laboratory pure cultures and industrial activated sludge samples suggest that the presence of genes associated with dioxane monooxygenase, propane monooxygenase, alcohol dehydrogenase, and aldehyde dehydrogenase are promising indicators of 1,4-dioxane biotransformation; however, gene abundance was insufficient to predict actual biodegradation. A time course gene expression analysis of dioxane and propane monooxygenases in Pseudonocardia dioxanivorans CB1190 and mixed communities in wastewater samples revealed important associations with the rates of 1,4-dioxane removal. In addition, transcripts of alcohol dehydrogenase and aldehyde dehydrogenase genes were upregulated during biodegradation, although only the aldehyde dehydrogenase was significantly correlated with 1,4-dioxane concentrations. Expression of the propane monooxygenase demonstrated a time-dependent relationship with 1,4-dioxane biodegradation in P. dioxanivorans CB1190, with increased expression occurring after over 50% of the 1,4-dioxane had been removed. While the fraction of P. dioxanivorans CB1190-like bacteria among the total bacterial population significantly increased with decrease in 1,4-dioxane concentrations in wastewater treatment samples undergoing active biodegradation, the abundance and expression of monooxygenase-based biomarkers were better predictors of 1,4-dioxane degradation than taxonomic 16S rRNA genes. This study illustrates that specific bacterial monooxygenase and dehydrogenase gene targets together can serve as effective biomarkers for 1,4-dioxane biodegradation in the environment.

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Section: Discussionmentioning

confidence: 98%

Identification of Biomarker Genes To Predict Biodegradation of 1,4-Dioxane

Gedalanga

Pornwongthong

Mora³

et al. 2014

Appl Environ Microbiol

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“…NBB4 could efficiently degrade chlorinated aliphatic compounds (1,2dichloroethane, vinyl chloride and cis-dichloroethane; Le and Coleman 2011). In turn, Mycobacterium vaccae JOB5 oxidized several cyclic ethers (Lan et al 2013). Vila and Grifoll (2009) documented removal of alkyl-PAHs from the Prestige tanker fuel by Mycobacterium sp.…”

Section: Bacteria Able To Metabolize Both N-alkanes and Aromatic Hydrmentioning

confidence: 99%

Aerobic bacteria degrading both n-alkanes and aromatic hydrocarbons: an undervalued strategy for metabolic diversity and flexibility

2018

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Environmental pollution with petroleum toxic products has afflicted various ecosystems, causing devastating damage to natural habitats with serious economic implications. Some crude oil components may serve as growth substrates for microorganisms. A number of bacterial strains reveal metabolic capacities to biotransform various organic compounds. Some of the hydrocarbon degraders are highly biochemically specialized, while the others display a versatile metabolism and can utilize both saturated aliphatic and aromatic hydrocarbons. The extended catabolic profiles of the latter group have been subjected to systematic and complex studies relatively rarely thus far. Growing evidence shows that numerous bacteria produce broad biochemical activities towards different hydrocarbon types and such an enhanced metabolic potential can be found in many more species than the already well-known oil-degraders. These strains may play an important role in the removal of heterogeneous contamination. They are thus considered to be a promising solution in bioremediation applications. The main purpose of this article is to provide an overview of the current knowledge on aerobic bacteria involved in the mineralization or transformation of both n-alkanes and aromatic hydrocarbons. Variant scientific approaches enabling to evaluate these features on biochemical as well as genetic levels are presented. The distribution of multidegradative capabilities between bacterial taxa is systematically shown and the possibility of simultaneous transformation of complex hydrocarbon mixtures is discussed. Bioinformatic analysis of the currently available genetic data is employed to enable generation of phylogenetic relationships between environmental strain isolates belonging to the phyla Actinobacteria, Proteobacteria, and Firmicutes. The study proves that the co-occurrence of genes responsible for concomitant metabolic bioconversion reactions of structurally-diverse hydrocarbons is not unique among various systematic groups.

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“…PG-3-5 greatly enhanced the velocity of butane degradation, demonstrating that the enhanced degradation velocity was the result of the cooperation between the BOB strains and the butanol-oxidizing strain. Since BOB and other hydrocarbon-degrading bacteria play an important role in the elimination of hydrocarbon contamination caused by leaching during petroleum extraction [20,21], our findings about the syntrophic relationships between the alkane and alcohol-oxidizing bacteria might be important for improving the bioremediation efficiency of hydrocarbon contamination.…”

Section: Discussionmentioning

confidence: 92%

“…The bmoX gene encodes the alpha hydroxylase subunit of butane monooxygenase, which is the most conservative gene segment [14,19]. The degradation of other organic pollutants by BOBs has also been studied [20,21].…”

Section: Introductionmentioning

confidence: 99%

Syntrophic Interactions Within a Butane-Oxidizing Bacterial Consortium Isolated from Puguang Gas Field in China

Zhang

Deng

Shen³

et al. 2016

Microb Ecol

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Butane oxidation by the hydrocarbon degradation bacteria has long been described, but little is known about the microbial interaction in this process. To investigate this interaction, the efficiency of butane oxidation was estimated in monocultures and co-cultures of six strains of butane-oxidizing bacteria (BOB) and a butanol-oxidizing strain. Results showed that the butane degradation velocity was at least 26 times higher in the co-culture of the seven strains (228.50 nmol h(-1)) than in the six individual monocultures (8.71 nmol h(-1)). Gas chromatographic analysis of metabolites in the cultures revealed the accumulation of butanol in the monocultures of BOB strains but not in the co-culture with the butanol-oxidizing strain. These results evidenced a novel syntrophic association between BOB and butanol-oxidizing bacteria in the butane oxidation. The BOB strains oxidized butane into butanol, but this activity was inhibited by the accumulated butanol in monocultures, whereas the removal of butanol by the butanol-oxidizing strain in co-culture could eliminate the suppression and improve the butane degradation efficiency. In the co-culture, both BOB and butanol-oxidizing bacteria could grow and the time needed for butane complete removal was shortened from more than 192 h to less than 4 h. The unsuppressed effect of the co-culture was also consistent with the results of reverse transcription quantitative real-time PCR (RT-qPCR) of bmoX gene because increased expression of this gene was detected during the syntrophic growth compared with that in monoculture, pointing to the upregulation of bmoX in the syntrophic interaction.

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Oxidation of Cyclic Ethers by Alkane‐Grown Mycobacterium vaccae JOB5

Abstract: In this study we investigated the cometabolic oxidation of six cyclic ethers by alkane-grownMycobacterium vaccae JOB5. These ethers include, among others, tetrahydrofuran (THF), 1, 1,

Cited by 23 publications

References 41 publications

Identification of Biomarker Genes To Predict Biodegradation of 1,4-Dioxane

Identification of Biomarker Genes To Predict Biodegradation of 1,4-Dioxane

Aerobic bacteria degrading both n-alkanes and aromatic hydrocarbons: an undervalued strategy for metabolic diversity and flexibility

Syntrophic Interactions Within a Butane-Oxidizing Bacterial Consortium Isolated from Puguang Gas Field in China

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