Oxidation of 2-keto-4-thiomethylbutyric acid (KTBA) by iron-binding compounds produced by the wood-decaying fungus Gloeophyllum trabeum

Abstract

The ability of iron‐binding compounds isolated from the brown‐rot fungus Gloeophyllum trabeum to carry out one‐electron oxidation reactions was established using a model substrate, 2‐keto‐4‐thiomethylbutyric acid (KTBA). The oxidation reaction was monitored by measuring the amount of ethylene produced from the substrate by gas chromatography. The extent of the reaction was found to be influenced by the concentration of the chelators, and by iron and manganese.

N-Glycoform Diversity of Cellobiohydrolase I from Penicillium decumbens and Synergism of Nonhydrolytic Glycoform in Cellulose Degradation

“…More than 60 years ago, Reese et al (28) first proposed that the C1 factor destroying the crystalline of cellulose is beneficial for the hydrolysis of cellulose by cellulase. In the course of exploring the mechanism of cellulose degradation, many studies discovered several materials with a similar function to C1, including swollenin from T. reesei (29), CDH from Phanerichaete chrysoporiu (30), HO ⅐ from T. reesei (31), and the short fiber-generating factor from T. pseudokoningi (32). However, the above materials are subject to much debate because of their unapparent synergism with commercial cellulases.…”

Degradation of cellulose by basidiomycetous fungi

“…Low-molecular-weight chelators of catecholate origin were isolated from white rot and brown rot fungi in the late 1980s and early 1990s (Fekete et al, 1989;Jellison et al, 1991;Chandhoke et al, 1992). The research was focused on the chelators produced by G. trabeum ('Gt chelators') that were of small molecular size (o 1000 Da) and, unlike enzymes, could penetrate through the wood cell wall matrix (Jellison et al, 1991;Goodell et al, 1997;Filley et al, 2002;Goodell et al, 2002).…”

Caracterização de compostos de baixa massa molar redutores de ferro produzidos por fungos e mediação da reação de fenton para degradação de polissacarídeos e lignina

“…Através de vários trabalhos, o grupo de Gao também propõe a participação de peptídeos de baixa massa molar na produção de radicais hidroxila via reação de Fenton, tanto em fungos de decomposição branca, quanto em fungos de decomposição parda (WANG; GAO, , 2003WANG et al, 2006;YANG et al, 2004 (CHANDHOKE et al, 1992;JELLISON et al, 1991;GOODELL et al, 1997;PASZCZYNSKI et al, 1999;GOODELL, 2001) e que são principalmente compostos aromáticos como os derivados de ácido hidroxifenilacético, de ácido hidroxi-benzóico e de hidroxi-benzeno . Goodell et al (1997) Uma quinona redutase dependente de NADH isolada do micélio de G.…”

“…trabeum é a enzima responsável por reduzir quinona à hidroquinona, e usa 2,5-dimetoxi-1,4-benzoquinona e 4,5-dimetoxi-1,2-benzoquinona como aceptores de elétrons (COHEN et al, 2004;QI;JELLISON, 2004 (COHEN et al, 2002(COHEN et al, , 2004CHANDHOKE et al, 1992;GOODELL et al, 1997;JELLISON et al, 1991;JENSEN et al, 2001HAMMEL, 1998;PASZCZYNSKI et al, 1999;GOODELL, 2001;SUZUKI et al, 2006). Dessa forma, mais estudos são necessários para constatar se este sistema oxidativo de baixa massa molar envolvendo compostos redutores de ferro na geração de radicais hidroxila via reação de Fenton é comum entre os fungos de decomposição branca ou específico do gênero Gloeophyllum.…”