Oxidases, peroxidases and hydrogen peroxide: The suberin connection

Bernards, Mark A.; Summerhurst, D. Kristen; Razem, Fawzi A.

doi:10.1023/b:phyt.0000047810.10706.46

Cited by 79 publications

(60 citation statements)

References 124 publications

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“…A mass spectrum of one of the diferulates encountered is illustrated in Figure S13. These ferulate dimers are expected to be the products of the coupling of phenyl and/or phenoxy radicals, similar to the reactions proposed for the polymerization of lignin and the polyphenolic domain of suberin (Bernards, 2002; Razem and Bernards, 2002; Bernards et al ., 2004). …”

Section: Resultsmentioning

confidence: 99%

AtMYB41 activates ectopic suberin synthesis and assembly in multiple plant species and cell types

et al. 2014

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Suberin is a lipid and phenolic cell wall heteropolymer found in the roots and other organs of all vascular plants. Suberin plays a critical role in plant water relations and in protecting plants from biotic and abiotic stresses. Here we describe a transcription factor, AtMYB41 (At4g28110), that can activate the steps necessary for aliphatic suberin synthesis and deposition of cell wall-associated suberin-like lamellae in both Arabidopsis thaliana and Nicotiana benthamiana. Overexpression of AtMYB41 increased the abundance of suberin biosynthetic gene transcripts by orders of magnitude and resulted in the accumulation of up to 22 times more suberin-type than cutin-type aliphatic monomers in leaves. Overexpression of AtMYB41 also resulted in elevated amounts of monolignols in leaves and an increase in the accumulation of phenylpropanoid and lignin biosynthetic gene transcripts. Surprisingly, ultrastructural data indicated that overexpression led to the formation of suberin-like lamellae in both epidermal and mesophyll cells of leaves. We further implicate AtMYB41 in the production of aliphatic suberin under abiotic stress conditions. These results provide insight into the molecular-genetic mechanisms of the biosynthesis and deposition of a ubiquitous cell wall-associated plant structure and will serve as a basis for discovering the transcriptional network behind one of the most abundant lipid-based polymers in nature.

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Section: Resultsmentioning

confidence: 99%

AtMYB41 activates ectopic suberin synthesis and assembly in multiple plant species and cell types

et al. 2014

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“…These data support earlier literature describing the histochemical detection of SPPD and SPAD components and in which a temporal as well as a spatial relationship between these two suberin domains was suggested (reviewed in Bernards and Lewis, 1998). These histochemical analyses provided the basis for a model of suberin macromolecular assembly in which specific phenolic compounds that accumulate in response to a wounding event are first polymerized into the SPPD, within the primary cell wall; this event is closely followed by the biosynthesis of SPAD components and their assembly into a multi-lammelar structure between the cell wall and plasma membrane (see Bernards, 2002, Bernards et al, 2003. Our metabolite profiling data support this model and begin to define key metabolites (e.g., ferulic acid) in the process.…”

Section: New Insights Into Suberization and The Overall Wound Responsmentioning

confidence: 99%

Metabolite profiling of potato (Solanum tuberosum L.) tubers during wound-induced suberization

Yang

Bernards

2007

Metabolomics

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Suberin is a specific cell wall-associated biopolymer characterized by the deposition of both a poly(phenolic) domain (SPPD) associated with the cell wall, and a poly(aliphatic) domain (SPAD) thought to be deposited between the cell wall and plasma membrane. In planta, suberin functions to prevent plants from desiccation and pathogen attack. Although the chemical identity of the monomeric components of the SPPD and SPAD are well known, their concerted biosynthesis and assembly into the suberin macromolecule is poorly understood. To expand our knowledge of suberin biosynthesis, a GC/MS-based metabolite profiling study was conducted, using wound healing potato (Solanum tuberosum L.) tubers as a model system. A time series of both non-polar and polar metabolite profiles were created, yielding a broad-based, dynamic picture of wound-induced metabolism, including suberization. Principal component analysis revealed a separation of metabolite profiles according to different suberization stages, with clear temporal differences emerging in the non-polar and polar profiles. In the non-polar profiles, suberin-associated aliphatics contributed the most to cluster formation, while a broader range of metabolites (including organic acids, sugars, amino acids and phenylpropanoids) influenced cluster formation amongst polar profiles. Pair-wise correlation analysis revealed strong correlations between known suberin-associated compounds, as well as between suberin-associated compounds and several un-identified metabolites in the profiles. These data may help to identify additional, as yet unknown metabolites associated with suberization process.

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“…The enzymes involved in suberin biosynthesis consist of elongases, hydroxylases and peroxidases (Bernards et al 2004;Franke et al 2005;Hofer et al 2008). However, their expression and regulation under stress conditions have not been extensively characterized.…”

Section: Introductionmentioning

confidence: 99%

The role of root apoplastic transport barriers in salt tolerance of rice (Oryza sativa L.)

Krishnamurthy

Ranathunge

Franke

et al. 2009

Planta

211

186

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Increasing soil salinity reduces crop yields worldwide, with rice being particularly affected. We have examined the correlation between apoplastic barrier formation in roots, Na+ uptake into shoots and plant survival for three rice (Oryza sativa L.) cultivars of varying salt sensitivity: the salt-tolerant Pokkali, moderately tolerant Jaya and sensitive IR20. Rice plants grown hydroponically or in soil for 1 month were subjected to both severe and moderate salinity stress. Apoplastic barriers in roots were visualized using fluorescence microscopy and their chemical composition determined by gas chromatography and mass spectrometry. Na+ content was estimated by flame photometry. Suberization of apoplastic barriers in roots of Pokkali was the most extensive of the three cultivars, while Na+ accumulation in the shoots was the least. Saline stress induced the strengthening of these barriers in both sensitive and tolerant cultivars, with increase in mRNAs encoding suberin biosynthetic enzymes being detectable within 30 min of stress. Enhanced barriers were detected after several days of moderate stress. Overall, more extensive apoplastic barriers in roots correlated with reduced Na+ uptake and enhanced survival when challenged with high salinity.

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Oxidases, peroxidases and hydrogen peroxide: The suberin connection

Cited by 79 publications

References 124 publications

AtMYB41 activates ectopic suberin synthesis and assembly in multiple plant species and cell types

AtMYB41 activates ectopic suberin synthesis and assembly in multiple plant species and cell types

Metabolite profiling of potato (Solanum tuberosum L.) tubers during wound-induced suberization

The role of root apoplastic transport barriers in salt tolerance of rice (Oryza sativa L.)

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