Oxidant Stress: The Role of the Glutathione Redox Cycle in Skin Preconditioning

Rees, Riley S.; Smith, David J.; Adamson, Belinda; Im, Michael J.; Hinshaw, Daniel B.

doi:10.1006/jsre.1995.1061

Cited by 21 publications

(19 citation statements)

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“…Although enhanced oxidative stress is believed to be a crucial mediator of lung injury, it may also modulate the process of subsequent wound healing. For example, oxidative stress has been shown to delay epithelial wound healing in the skin and the gastric mucosa [5,10,11]. However, it remains unclear whether oxidative stress modulates wound healing by ® broblasts.…”

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confidence: 99%

Apoptosis of Wound Fibroblasts Induced by Oxidative Stress

Takahashi

Aoshiba

Nagai

2002

Experimental Lung Research

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Irreversible lung parenchymal injury is usually healed by fibrosis, which depends on the abilities of fibroblasts to proliferate, migrate into the wound, and survive. Because the lung is frequently exposed to increased oxidative stress, which is thought to mediate apoptosis, we examined whether oxidative stress induces apoptosis in fibroblasts during wound healing. We performed an in vitro scratch wound assay where cultured fibroblast monolayers were exposed to H2O2 (10-500 microM) after artificial wounding. Apoptosis was evaluated by nuclear staining with Hoechst33342 or terminal deoxynucleotidyl transferase (TdT)-mediated nucleotide nick end-labeling (TUNEL). Intracellular oxidants were assessed with the peroxide-sensitive fluorochrome carboxydichlorodihydrofluorescein (CDCF). We found that repopulating fibroblasts at the wound margin, but not quiescent fibroblasts at the intact site, selectively underwent oxidant accumulation and apoptosis in response to H2O2 exposure. Some of the apoptotic cells had incorporated bromodeoxyuridine (BrdU), an indicator of proliferating cells. These results suggest that oxidative stress selectively induces apoptosis in fibroblasts that are stimulated to proliferate and/or migrate into the wound. Fibroblast apoptosis induced by oxidative stress during wound repopulation may be relevant to intractable wound healing.

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confidence: 99%

Apoptosis of Wound Fibroblasts Induced by Oxidative Stress

Takahashi

Aoshiba

Nagai

2002

Experimental Lung Research

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“…The ischemic condition that results from skin flap elevation may also progress due to various biochemical events [8,11,12,13]. The skin in our model was therefore under dual oxidant stress; stress caused by burns followed by flap elevation.…”

Section: Discussionmentioning

confidence: 99%

The use of superficial and deep partial-thickness burned tissue for flap fabrication

Borman¹,

Maral

Bilezikçi

et al. 2002

European Journal of Plastic Surgery

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When flaps are indicated for early wound closure in extensive burns, and there is limited unburned local tissue to design these, one option may be to use a partial-thickness burned area as a flap for construction in the acute phase. This study examined the proportions of superficial and deep partial-thickness burned skin that can safely be included in an island flap. Eighty male Wistar rats were divided into four groups, with varying extent of burning of the right epigastric flap site: group 1, 50% superficially burned; group 2, 100% superficially burned; group 3, 50% deeply burned; and group 4, 100% deeply burned. In each group the left epigastric flaps served as controls. The superficially burned flaps in groups 1 and 2 healed at 2-4 weeks, and the deeply burned flaps in groups 3 and 4 healed at 4-6 weeks postoperatively. In all cases hair grew on the burned flap sites, and the flaps healed in such a way that they were almost impossible to distinguish, even after the hair was shaved at week 8. It was observed that the superficially burned flaps healed perfectly without scarring, but small portions of the deeply burned flaps developed scars. In conclusion, superficially and deeply burned island flaps survived completely in this animal model, even when the entire flap surface was burned. In the future, under appropriate conditions the transfer of partial-thickness burned tissue as flaps may provide a good alternative for reconstruction of the acute burn wound.

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“…Glutathione was assayed as previously described [28]. Briefly, cells were trypsinized and counted using a hemacytometer before being suspended in a 2.5% sulfosalicylic acid, 0.2% Triton X-100 solution.…”

Section: Animal Model Of Ischemia-reperfusionmentioning

confidence: 99%