Oxaliplatin delivery via chitosan/vitamin E conjugate micelles for improved efficacy and MDR-reversal in breast cancer

Itoo, Asif Mohd; Paul, Milan; Ghosh, Balaram; Biswas, Swati

doi:10.1016/j.carbpol.2022.119108

Cited by 54 publications

(49 citation statements)

References 50 publications

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“…Then, the cells were washed with PBS 7.4 and fixed using 4% paraformaldehyde (500 μL/well) for 20 min under dark conditions, followed by staining using DAPI (10 μg/mL, 90 μL/well) and AO (100 μg/mL, 90 μL/well), visualized under a fluorescence microscope at 358 nm (blue channel for DAPI) and 480−490 nm (green channel for AO). 32 2.6.5. DNA Fragmentation.…”

Section: Cell Viability %mentioning

confidence: 99%

Dual-Drug-Loaded Topical Delivery of Photodynamically Active Lipid-Based Formulation for Combination Therapy of Cutaneous Melanoma

et al. 2023

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Topical administration of anti-cancer drugs along with photodynamically active molecules is a non-invasive approach, which stands to be a promising modality for treating aggressive cutaneous melanomas with the added advantage of high patient compliance. However, the efficiency of delivering drugs topically is limited by several factors, such as penetration of the drug across skin layers at the tumor site and limited light penetrability. In this study, curcumin, an active anti-cancer agent, and chlorin e6, a photoactivable molecule, were encapsulated into lipidic nanoparticles that produced reactive oxygen species (ROS) when activated at 665 nm by near-infrared (NIR) light. The optimized lipidic nanoparticle containing curcumin and chlorin e6 exhibited a particle size of less than 100 nm. The entrapment efficiency for both molecules was found to be 81%. The therapeutic efficacy of the developed formulation was tested on B16F10 and A431 cell lines via cytotoxicity evaluation, combination index, cellular uptake, nuclear staining, DNA fragmentation, ROS generation, apoptosis, and cell cycle assays under NIR irradiation (665 nm). Co-delivering curcumin and chlorin e6 exhibited higher cellular uptake, better cancer growth inhibition, and pronounced apoptotic events compared to the formulation having the free drug alone. The study results depicted that topical application of this ROS-generating dual-drug-loaded lipidic nanoparticles incorporated in SEPINEO gel achieved better permeation (80 ± 2.45%) across the skin, and exhibited the improved skin retention and a synergistic effect as well. The present work introduces photo-triggered ROS-generating dual-drug-based lipidic nanoparticles, which are simple and efficient to develop and exhibit synergistic therapeutic effects against cutaneous melanoma.

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Section: Cell Viability %mentioning

confidence: 99%

Dual-Drug-Loaded Topical Delivery of Photodynamically Active Lipid-Based Formulation for Combination Therapy of Cutaneous Melanoma

et al. 2023

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“…A micelle is a group of amphoteric surfactant molecules that autonomously assemble in water to form a (typically) spherical vesicle . Ms may thus be utilized to integrate lipophilic PSs for cancer (PDT).…”

Section: Nanotechnology-based Delivery Of Photosensitizersmentioning

confidence: 99%

“…A micelle is a group of amphoteric surfactant molecules that autonomously assemble in water to form a (typically) spherical vesicle. 133 Ms may thus be utilized to integrate lipophilic PSs for cancer (PDT). Since the Ms core is hydrophobic, hydrophobic PSs may be sequestered there until a drug delivery system releases them.…”

Section: Nanotechnology-based Delivery Of Photosensitizersmentioning

confidence: 99%

“…Cancer is one of the most worrisome illnesses, particularly in affluent nations. Nearly 8 million people die from cancer each year, contributing significantly to the burden of cancer-related mortality and morbidity worldwide. − Chemotherapy, surgery, radiotherapy, and, more recently, small molecule-based treatments and immunotherapy, as well as a combination of these approaches, are the current clinical approaches for cancer therapy. Surgery is typically the first course of treatment; however, if metastasis has already taken place, it may not be the most appropriate treatment option.…”

Section: Introductionmentioning

confidence: 99%

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Nanotherapeutic Intervention in Photodynamic Therapy for Cancer

et al. 2022

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The clinical need for photodynamic therapy (PDT) has been growing for several decades. Notably, PDT is often used in oncology to treat a variety of tumors since it is a low-risk therapy with excellent selectivity, does not conflict with other therapies, and may be repeated as necessary. The mechanism of action of PDT is the photoactivation of a particular photosensitizer (PS) in a tumor microenvironment in the presence of oxygen. During PDT, cancer cells produce singlet oxygen (1O2) and reactive oxygen species (ROS) upon activation of PSs by irradiation, which efficiently kills the tumor. However, PDT’s effectiveness in curing a deep-seated malignancy is constrained by three key reasons: a tumor’s inadequate PS accumulation in tumor tissues, a hypoxic core with low oxygen content in solid tumors, and limited depth of light penetration. PDTs are therefore restricted to the management of thin and superficial cancers. With the development of nanotechnology, PDT’s ability to penetrate deep tumor tissues and exert desired therapeutic effects has become a reality. However, further advancement in this field of research is necessary to address the challenges with PDT and ameliorate the therapeutic outcome. This review presents an overview of PSs, the mechanism of loading of PSs, nanomedicine-based solutions for enhancing PDT, and their biological applications including chemodynamic therapy, chemo-photodynamic therapy, PDT–electroporation, photodynamic–photothermal (PDT–PTT) therapy, and PDT–immunotherapy. Furthermore, the review discusses the mechanism of ROS generation in PDT advantages and challenges of PSs in PDT.

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“…Given the highly promising results achieved with glycol chitosan (GC) for resistant cancer therapy, one study reported GC NP encapsulation of both chemotherapeutics (DOX) and siRNA (BCL-2) to achieve maximal efficacy to overcome MDR ( 41 ). Likewise, self-assembly of chitosan/vitamin E conjugate into micelle is used to entrap oxaliplatin (OXPt) to overcome conventional OXPt-mediated drug resistance/nephrotoxicity in breast cancer ( 42 ). Also, folic acid-functionalized chitosan nanocarrier is developed for codelivery of DOX and oleanolic acid (OA), to form a copolymer with self-assembly into 180-nm NPs in water.…”

Section: Chitosan: Inherent Propertiesmentioning

confidence: 99%

Tumor microenvironment penetrating chitosan nanoparticles for elimination of cancer relapse and minimal residual disease

et al. 2022

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Chitosan and its derivatives are among biomaterials with numerous medical applications, especially in cancer. Chitosan is amenable to forming innumerable shapes such as micelles, niosomes, hydrogels, nanoparticles, and scaffolds, among others. Chitosan derivatives can also bring unprecedented potential to cross numerous biological barriers. Combined with other biomaterials, hybrid and multitasking chitosan-based systems can be realized for many applications. These include controlled drug release, targeted drug delivery, post-surgery implants (immunovaccines), theranostics, biosensing of tumor-derived circulating materials, multimodal systems, and combination therapy platforms with the potential to eliminate bulk tumors as well as lingering tumor cells to treat minimal residual disease (MRD) and recurrent cancer. We first introduce different formats, derivatives, and properties of chitosan. Next, given the barriers to therapeutic efficacy in solid tumors, we review advanced formulations of chitosan modules as efficient drug delivery systems to overcome tumor heterogeneity, multi-drug resistance, MRD, and metastasis. Finally, we discuss chitosan NPs for clinical translation and treatment of recurrent cancer and their future perspective.

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Oxaliplatin delivery via chitosan/vitamin E conjugate micelles for improved efficacy and MDR-reversal in breast cancer

Cited by 54 publications

References 50 publications

Dual-Drug-Loaded Topical Delivery of Photodynamically Active Lipid-Based Formulation for Combination Therapy of Cutaneous Melanoma

Dual-Drug-Loaded Topical Delivery of Photodynamically Active Lipid-Based Formulation for Combination Therapy of Cutaneous Melanoma

Nanotherapeutic Intervention in Photodynamic Therapy for Cancer

Tumor microenvironment penetrating chitosan nanoparticles for elimination of cancer relapse and minimal residual disease

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