Oxalic acid degradation in wood-rotting fungi. Searching for a new source of oxalate oxidase

Abstract

Oxalate oxidase (EC 1.2.3.4) is an oxalate-decomposing enzyme predominantly found in plants but also described in basidiomycete fungi. In this study, we investigated 23 fungi to determine their capability of oxalic acid degradation. After analyzing their secretomes for the products of the oxalic acid-degrading enzyme activity, three groups were distinguished among the fungi studied. The first group comprised nine fungi classified as oxalate oxidase producers, as their secretome pattern revealed an increase in the hydrogen peroxide concentration, no formic acid, and a reduction in the oxalic acid content. The second group of fungi comprised eight fungi described as oxalate decarboxylase producers characterized by an increase in the formic acid level associated with a decrease in the oxalate content in their secretomes. In the secretomes of the third group of six fungi, no increase in formic acid or hydrogen peroxide contents was observed but a decline in the oxalate level was found. The intracellular activity of OXO in the mycelia of Schizophyllum commune, Trametes hirsuta, Gloeophyllum trabeum, Abortiporus biennis, Cerrena unicolor, Ceriosporopsis mediosetigera, Trametes sanguinea, Ceriporiopsis subvermispora, and Laetiporus sulphureus was confirmed by a spectrophotometric assay.

Oxygen-dependent biofilm dynamics in leaf decay: an in vitro analysis

“…Furthermore, Trametes strains secrete extracellular fatty acid droplets 49 and corrode apatite under glucose-limited conditions 50 . Graz et al 2023 confirmed the oxalic acid-degrading activity of Trametes and Laetiporus 51 . Oxalic acid is commonly secreted by the majority of fungi during the wood decay process, and it is linked with the ability to chelate metals 51 , 52 .…”

“…Graz et al 2023 confirmed the oxalic acid-degrading activity of Trametes and Laetiporus 51 . Oxalic acid is commonly secreted by the majority of fungi during the wood decay process, and it is linked with the ability to chelate metals 51 , 52 . The endophytic fungi Sarocladium and Penicillium were enriched in the biofilms according to Deseq2.…”