Oxalate‐Degrading <i>Enterococcus faecalis</i>

Hokama, Seitetsu; Honma, Yasuko; Toma, Claudia; Ogawa, Yoshihide

doi:10.1111/j.1348-0421.2000.tb02489.x

Cited by 80 publications

(61 citation statements)

References 28 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…An increase in intestinal absorption of oxalate is known to lead to hyperoxaluria, with a significantly enhanced risk of urinary stone formation (10,15,47). Several studies have already demonstrated the presence of oxalate-degrading bacteria in the human intestine (2,20,23) and their ability to control oxalate levels by influencing intestinal absorption of dietary oxalate. Identification of intestinal bacteria with oxalate-degrading activity can offer unique opportunities to provide this capacity to individuals suffering from an increased body burden of oxalate and oxalate-associated disorders.…”

Section: Discussionmentioning

confidence: 99%

Characterization and Heterologous Expression of the Oxalyl Coenzyme A Decarboxylase Gene from Bifidobacterium lactis

Federici

Vitali

Gotti

et al. 2004

Appl Environ Microbiol

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Characterization and Heterologous Expression of the Oxalyl Coenzyme A Decarboxylase Gene from Bifidobacterium lactis

Federici

Vitali

Gotti

et al. 2004

Appl Environ Microbiol

View full text Add to dashboard Cite

“…Other oxalate-degrading bacteria isolated from the human GIT include Eubacterium lentum (22) and Enterococcus faecalis (20 . They observed a reduction in the excreted oxalate in the patients and showed that L. acidophilus and S. thermophilus could reduce oxalate concentrations in vitro, even when their growth was partially inhibited by this compound.…”

Section: Discussionmentioning

confidence: 99%

Transcriptional and Functional Analysis of Oxalyl-Coenzyme A (CoA) Decarboxylase and Formyl-CoA Transferase Genes from Lactobacillus acidophilus

Azcarate‐Peril

Bruno-Bárcena²,

Hassan³

et al. 2006

Appl Environ Microbiol

View full text Add to dashboard Cite

Oxalic acid is found in dietary sources (such as coffee, tea, and chocolate) or is produced by the intestinal microflora from metabolic precursors, like ascorbic acid. In the human intestine, oxalate may combine with calcium, sodium, magnesium, or potassium to form less soluble salts, which can cause pathological disorders such as hyperoxaluria, urolithiasis, and renal failure in humans. In this study, an operon containing genes homologous to a formyl coenzyme A transferase gene (frc) and an oxalyl coenzyme A decarboxylase gene (oxc) was identified in the genome of the probiotic bacterium Lactobacillus acidophilus. Physiological analysis of a mutant harboring a deleted version of the frc gene confirmed that frc expression specifically improves survival in the presence of oxalic acid at pH 3.5 compared with the survival of the wild-type strain. Moreover, the frc mutant was unable to degrade oxalate. These genes, which have not previously been described in lactobacilli, appear to be responsible for oxalate degradation in this organism. Transcriptional analysis using cDNA microarrays and reverse transcription-quantitative PCR revealed that mildly acidic conditions were a prerequisite for frc and oxc transcription. As a consequence, oxalate-dependent induction of these genes occurred only in cells first adapted to subinhibitory concentrations of oxalate and then exposed to pH 5.5. Where genome information was available, other lactic acid bacteria were screened for frc and oxc genes. With the exception of Lactobacillus gasseri and Bifidobacterium lactis, none of the other strains harbored genes for oxalate utilization.

show abstract

“…One mechanism by which mammalian herbivores may overcome PSCs is to host microbes capable of degrading such toxins (7). Dozens of species of bacteria capable of metabolizing dietary PSCs have been isolated from the guts of mammals, suggesting a long-term coevolution between hosts and their microbiotas (8)(9)(10)(11)(12). Additionally, the structure of the gut microbiota can be altered significantly by exposure to dietary PSCs and other xenobiotics, which is indicative of short-term ecological interactions between the diet and the microbiota (2,13).…”

mentioning

confidence: 99%

“…Despite its prevalence and toxicity, mammals do not produce the enzymes capable of metabolizing oxalate (22,23). However, oxalate-degrading bacteria are widely distributed within the guts of mammals (8,11,(24)(25)(26)(27)(28)(29).…”

mentioning

confidence: 99%

The Gastrointestinal Tract of the White-Throated Woodrat (Neotoma albigula) Harbors Distinct Consortia of Oxalate-Degrading Bacteria

Miller

Kohl

Dearing

2014

Appl Environ Microbiol

View full text Add to dashboard Cite

The microbiota inhabiting the mammalian gut is a functional organ that provides a number of services for the host. One factor that may regulate the composition and function of gut microbial communities is dietary toxins. Oxalate is a toxic plant secondary compound (PSC) produced in all major taxa of vascular plants and is consumed by a variety of animals. The mammalian herbivore Neotoma albigula is capable of consuming and degrading large quantities of dietary oxalate. We isolated and characterized oxalate-degrading bacteria from the gut contents of wild-caught animals and used high-throughput sequencing to determine the distribution of potential oxalate-degrading taxa along the gastrointestinal tract. Isolates spanned three genera: Lactobacillus, Clostridium, and Enterococcus. Over half of the isolates exhibited significant oxalate degradation in vitro, and all Lactobacillus isolates contained the oxc gene, one of the genes responsible for oxalate degradation. Although diverse potential oxalate-degrading genera were distributed throughout the gastrointestinal tract, they were most concentrated in the foregut, where dietary oxalate first enters the gastrointestinal tract. We hypothesize that unique environmental conditions present in each gut region provide diverse niches that select for particular functional taxa and communities.

show abstract

Oxalate‐Degrading Enterococcus faecalis

Cited by 80 publications

References 28 publications

Characterization and Heterologous Expression of the Oxalyl Coenzyme A Decarboxylase Gene from Bifidobacterium lactis

Characterization and Heterologous Expression of the Oxalyl Coenzyme A Decarboxylase Gene from Bifidobacterium lactis

Transcriptional and Functional Analysis of Oxalyl-Coenzyme A (CoA) Decarboxylase and Formyl-CoA Transferase Genes from Lactobacillus acidophilus

The Gastrointestinal Tract of the White-Throated Woodrat (Neotoma albigula) Harbors Distinct Consortia of Oxalate-Degrading Bacteria

Contact Info

Product

Resources

About