Oxalate Decarboxylase from Collybia velutipes

Abstract

Oxalic acid is present as nutritional stress in many crop plants like Amaranth and Lathyrus. Oxalic acid has also been found to be involved in the attacking mechanism of several phytopathogenic fungi. A full-length cDNA for oxalate decarboxylase, an oxalate-catabolizing enzyme, was isolated by using 5-rapid amplification of cDNA ends-polymerase chain reaction of a partial cDNA as cloned earlier from our laboratory (

Oxalate decarboxylase of the white-rot fungus Dichomitus squalens demonstrates a novel enzyme primary structure and non-induced expression on wood and in liquid cultures

“…ODC is a relevant enzyme for biotechnological applications due to its ability to specifically decompose oxalic acid, with prospects from process industry to diagnostics. So far, only one fungal ODC has been completely cloned and enzymically described, from Flammulina (Collybia) velutipes (Mehta & Datta, 1991;Kesarwani et al, 2000). To add to the pool of fungal ODCs, we focused on identifying the gene and enzyme from D. squalens, which showed high ODC activity in our previous screening study (Mäkelä et al, 2002).…”

“…ODCs from several species of basidiomycetous and ascomycetous fungi have been reported (Emiliani & Bekes, 1964;Magro et al, 1988;Mehta & Datta, 1991;Dutton et al, 1994;Micales, 1997;Kathiara et al, 2000;Mäkelä et al, 2002), but so far only one basidiomycetous ODC enzyme, from the whiterot fungus Flammulina (Collybia) velutipes has been thoroughly characterized, at both the gene and protein level (Mehta & Datta, 1991;Kesarwani et al, 2000).…”

“…Other applications have utilized ODC in, for example, assays of oxalic acid concentration in clinical and food samples. The construction of transgenic odc-expressing crop plants can (i) improve their resistance against certain oxalic-acidsecreting plant-pathogenic fungi and (ii) by reducing their oxalic acid content, make them less toxic to humans and herbivores (Kesarwani et al, 2000;Dias et al, 2006). In a search for a treatment for excessive excretion of urinary oxalate (hyperoxaluria), oral therapy with a crystalline cross-linked formulation of ODC has been shown to reduce symptoms in experiments with mice (Grujic et al, 2009).…”

Silenciamento gênico induzido pelo hospedeiro (HIGS) do gene da quitina sintase em Sclerotinia sclerotiorum

“…No caso específico do mofo branco, o controle biológico mais conhecido utilizado é o fungo do gênero Trichoderma (Geraldine et al, 2013) (Kesarwani et al, 2000;Donaldson et al, 2001;Livingstone et al, 2005;Dias et al, 2006;Dong et al, 2008;Walz et al, 2008;. Outra alternativa é o uso da estratégia de silenciamento gênico induzido pelo hospedeiro (explicada detalhadamente mais a frente), a qual tem sido utilizada para alcançar resistência a fungos biotróficos, tais como do Erysiphe blumeria em cevada e trigo , Puccinia striiformis no trigo , Puccinia triticina no trigo , Fusarium oxysporum em banana e Bremia lactucae em alface (Govindarajulu et al, 2014).…”

“…The main strategy used so far consists of the expression of genes encoding enzymes capable of degrading oxalic acid, such as oxalate oxidases and oxalate decarboxylases, which have produced enhanced resistance to S. sclerotiorum (Kesarwani et al, 2000;Dias et al, 2006;Dong et al, 2008;. Another alternative is the use of host-induced gene silencing (HIGS) methods, which exploit the silencing effect of small interfering RNA (siRNA) signals in interacting organisms, and involves host expression of siRNA-generating constructs directed against genes in associated pathogens, parasites or symbionts (Knip et al, 2014).…”