OXA-258 from Achromobacter ruhlandii: a Species-Specific Marker

Abstract

A new bla OXA-258 gene is described as a species-specific taxonomic marker for Achromobacter ruhlandii isolates (all recovered from cystic fibrosis patients). Even though OXA-258 differs from OXA-114 variants, isolates could be misidentified as A. xiloxosidans by the amplification of an inner fragment from the OXA-coding gene. A robust identification of A. ruhlandii can be achieved by sequencing this single OXA gene, as well as by a more laborious recently proposed multilocus sequence-typing (MLST) scheme.

Characterization of Achromobacter Species in Cystic Fibrosis Patients: Comparison of bla OXA-114 PCR Amplification, Multilocus Sequence Typing, and Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry

“…Two isolates (isolates 4530 and 7393) were negative for bla OXA . As observed by Papalia et al (2), in our study not only A. xylosoxidans but also A. ruhlandii yielded positive amplification for the A. xylosoxidans species-specific marker bla . Amplification of the inner fragment without sequence analysis, as initially described by Turton et al (9), thus may result in the misidentification of some Achromobacter species; by this criterion, we would have identified 26 isolates instead of 17 as A. xylosoxidans.…”

“…The amplification of an inner fragment of the bla OXA-114 gene has been proposed for A. xylosoxidans identification (9). Furthermore, the presence of bla , bla , and bla OXA-243 has been detected in Achromobacter ruhlandii, Achromobacter dolens, and Achromobacter insuavis, respectively (2,3). Multilocus sequence typing (MLST) schemes that increase the accuracy of the characterization and identification of Achromobacter strains and species have also been developed (10,11).…”

“…Most Achromobacter clinical isolates are designated as Achromobacter xylosoxidans; however, the commonly used phenotypic tests are not reliable (1)(2)(3). Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis is used in clinical microbiology laboratories as an emerging technology for bacterial species identification (4)(5)(6)(7)(8).…”

Characterisation of OXA-258 enzymes and AxyABM efflux pump in Achromobacter ruhlandii

“…A. ruhlandii is considered an opportunistic pathogen known to be resistant to a wide range of antibiotics, however, the knowledge about the resistance mechanisms is limited. In a previous study we described two allelic variants of a new class D β-lactamase named OXA-258, which constitutes a naturally occurring β-lactamase in A. ruhlandii (5). OXA-258a differs from OXA-114a of A. xylosoxidans by 40 amino acids, displaying 85% identity (6).…”

Draft genome sequences of four Achromobacter ruhlandii strains isolated from cystic fibrosis patients

“…2012a; http://pubmlst.org/achromobacter/). Furthermore, the species specific marker genes for A. xylosoxidans ( bla OXA-114 ) and for A. ruhlandii ( bla OXA-258 ) were amplified and sequenced to confirm species assignment (Turton et al 2011, Papalia et al 2013). Accordingly, the sequences from all four strains showed identity with bla oxa-258 .Strains 6241, 7863, 7022 and 8173 were assigned STs 35, 204, 36 and 35, respectively.…”