Ox peripheral nerve myelin membrane. Purification and partial characterization of two basic proteins

Abstract

SUMMARYTwo basic proteins were purified from the peripheral nervous system. The isolation was achieved by (I) delipidation with chloroform-butanol mixtures, dry acetone, and dry ether, (2) acid extraction at pH 2 and then (3) dialysis against distilled water, lyophilization, and solubilization in pH-Io.7 buffer. Further purification steps included: (4) ion exchange chromatography on QAE-Sephadex G-25 and (5) gel filtration on Sephadex G-75. Each of the two basic proteins was shown to be homogeneous by disc electrophoresis at pH 2.0, 4.3, and 9.2.The acid extractable proteins of an isolated fraction from peripheral nerve were studied by using gel filtration and disc electrophoresis techniques. Two basic proteins were also recovered in this case. These proteins gave the same elution volumes and the same electrophoretic mobility as the basic proteins recovered from peripheral nerve. Myelin was extracted with butanol and 72-86 % of the myelin proteins were recovered as soluble lipoproteins. They were compared to the acid extractable proteins.

Immunological relationship between acetylcholine receptor and thymus: a possible significance in myasthenia gravis.

“…No stimulation of lymphocytes from the above rabbits was observed when they were cultured with any of the following antigens: basic proteins of bovine central (26) and peripheral (27) nerve tissue, human aqueous muscle extract, and hen egg white lysozyme (a nonrelated protein). The stimulation indices obtained with the mitogens phytohemagglutinin and concanavalin A were about 30.…”

Experimental allergic neuritis induced by galactocerebroside

“…In experimental allergic encephalomyelitis (EAE), the central nervous system (CNS) cohnterpart of EAN, the encephalitogen is the myelip basic protein. PNS myelin has two basic proteins, P1 and P2 [ l l , 13,191, and the latter (called P,L by London [35] and BF by Uyemura et al [66]) has been suggested to be the neuritogen [l, 2, 11, 291. Brostoff et al [12] have reported that a neuritogenic determinant is present within a peptide representing the NH,-terminal 2 1 amino acids of P2 [12].…”

Immunology of Guillain-Barré syndrome

“…It was denoted P2 or P2L by London [72] and by Abramsky and London [1,2] or P~ by Brostoff and Eylar [23]. Two basic proteins were found in myelin of bovine, rabbit, guinea pig, and human peripheral nerves.…”