Ovum pick up, intracytoplasmic sperm injection and somatic cell nuclear transfer in cattle, buffalo and horses: from the research laboratory to clinical practice

Establishment of conditions for ovum pick up and IVM of jennies oocytes toward the setting up of efficient IVF and in vitro embryos culture procedures in donkey (Equus asinus)

“…Then, transvaginal ultrasound-guided follicular puncture was developed in the mare [40,41]. Since then, the OPU technique has been extensively used in the equine [11][12][13][14][15][16][17][18][19][20][21]. The mean number of follicles punctured per mare per session ranges from two to 12 with an effect of mare [19], breed (lower number in pony mares compared with saddle mares) [12], the estrous cycle stage [14,15] and season [17].…”

“…The first step is the collection of several immature oocytes on a donor female using ovum pick up (OPU). The OPU technique has been widely used in the equine [11][12][13][14][15][16][17][18][19][20][21]. However, to our knowledge, OPU has never been described in jennies.…”

Animal Reproduction Technologies—Future Perspectives

“…It is rapidly outpacing conventional superovulation for ET, having increased more than ten-fold since the turn of century [26]. The technique uses ultrasound-guided follicular aspiration to recover oocytes from pre-ovulatory follicles in situ, and can even be used on pregnant cows.…”

Challenges and perspectives to enhance cattle production via in vitro techniques: focus on epigenetics and cell-secreted vesicles

“…Since then, a flurry of papers were published showing the effect of HDACis on SCNT in different species (MONTEIRO et al, 2010). The results are promising, but in some species such as bovine, remains highly controversial (AKAGI et al, 2013;GALLI et al, 2014). The majority of reports have addressed the effects on pre-implantation stages only.…”