Ovule identity mediated by pre-mRNA processing in Arabidopsis

Rodríguez-Cazorla, Encarnación; Ortuño-Miquel, Samanta; Candela, Héctor; Bailey-Steinitz, Lindsay J.; Yanofsky, Martin F.; Martı́nez-Laborda, Antonio; Ripoll, Juan-José; Vera, Antonio

doi:10.1371/journal.pgen.1007182

Cited by 18 publications

(33 citation statements)

References 124 publications

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“…We compared SHP2::GUS and STK::GUS expression in the WT and in h1h2P backgrounds, respectively. As observed previously (Pinyopich et al, 2003;Losa et al, 2010;Rodr ıguez-Cazorla et al, 2018), SHP2 and STK were broadly expressed in the WT both in placental tissue and ovules ( Fig. 2a,c), whereas in the transformed ovules of h1h2P, SHP2::GUS activity was stronger in the proximal portions, particularly in the stalk-like structure connecting to the placenta.…”

Section: Resultssupporting

confidence: 84%

Dissection of the Arabidopsis HUA‐PEP gene activity reveals that ovule fate specification requires restriction of the floral A‐function

et al. 2020

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Ovules are essential for sexual plant reproduction and seed formation, and are fundamental for agriculture. However, our understanding of the molecular mechanisms governing ovule development is far from complete. In Arabidopsis, ovule identity is determined by homeotic MADS-domain proteins that define the floral C-(AG) and D-(SHP1/SHP2, STK) functions. Pre-mRNA processing of these genes is critical and mediated by HUA-PEP activity, composed of genes encoding RNA-binding proteins. In strong hua-pep mutants, functional transcripts for C-and D-function genes are reduced, resulting in homeotic transformation of ovules. Thus, hua-pep mutants provide an unique sensitized background to study ovule morphogenesis when C-and D-functions are simultaneously compromised. We found that hua-pep ovules are morphologically sepaloid and show ectopic expression of the homeotic class-A gene AP1. Inactivation of AP1 or AP2 (A-function genes) in hua-pep mutants reduced homeotic conversions, rescuing ovule identity while promoting carpelloid traits in transformed ovules. Interestingly, increased AG dosage led to similar results. Our findings strongly suggest that HUA-PEP activity is required for correct C and D floral functions, which in turn prevents ectopic expression of class-A genes in ovules for their proper morphogenesis, evoking the classic A-C antagonism of the ABC model for floral organ development.

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Section: Resultssupporting

confidence: 84%

Dissection of the Arabidopsis HUA‐PEP gene activity reveals that ovule fate specification requires restriction of the floral A‐function

et al. 2020

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“…The assays described above, together with experimental data generated previously, show a direct positive correlation between seed-set and fruit valve elongation (60)(61)(62)72). Several studies in Arabidopsis and other species suggest that right after fertilization a cascade of signaling responses initiated in the ovule/seed trigger instructive responses that elicit fruit growth and valve elongation (50,64,75,76). To create a high-resolution spatiotemporal map at the cellular level of postfertilization valve cell epidermal cell growth, we developed a live-imaging platform we refer to as FLIP (fruit live-imaging platform).…”

Section: Fruit Elongation Is Triggered By Fertilization and Positivelmentioning

confidence: 75%

Growth dynamics of the Arabidopsis fruit is mediated by cell expansion

Ripoll

Zhu

Brocke

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

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Fruit have evolved a sophisticated tissue and cellular architecture to secure plant reproductive success. Postfertilization growth is perhaps the most dramatic event during fruit morphogenesis. Several studies have proposed that fertilized ovules and developing seeds initiate signaling cascades to coordinate and promote the growth of the accompanying fruit tissues. This dynamic process allows the fruit to conspicuously increase its size and acquire its final shape and means for seed dispersal. All these features are key for plant survival and crop yield. Despite its importance, we lack a high-resolution spatiotemporal map of how postfertilization fruit growth proceeds at the cellular level. In this study, we have combined live imaging, mutant backgrounds in which fertilization can be controlled, and computational modeling to monitor and predict postfertilization fruit growth in Arabidopsis. We have uncovered that, unlike leaves, sepals, or roots, fruit do not exhibit a spatial separation of cell division and expansion domains; instead, there is a separation into temporal stages with fertilization as the trigger for transitioning to cell expansion, which drives postfertilization fruit growth. We quantified the coordination between fertilization and fruit growth by imaging no transmitting tract (ntt) mutants, in which fertilization fails in the bottom half of the fruit. By combining our experimental data with computational modeling, we delineated the mobility properties of the seed-derived signaling cascades promoting growth in the fruit. Our study provides the basis for generating a comprehensive understanding of the molecular and cellular mechanisms governing fruit growth and shape.

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“…As illustrated above, in addition to transcription, post-transcriptional mechanisms are major determinants for flowering time regulation. The HUA-PEP activity is composed of a functionally versatile group of genes encoding RNA-binding proteins (RBP) that control pre-mRNA processing of the MADS-box genes AGAMOUS ( AG ) and its clade members SHATTERPROOF1 ( SHP1 ), SHP2 and SEEDSTICK ( STK ) 31–33 , crucial for flower and ovule morphogenesis 34,35 . Additionally, some HUA-PEP components also regulate FLC 36,37 .…”

Section: Introductionmentioning

confidence: 99%

Arabidopsis HUA ENHANCER 4 delays flowering by upregulating the MADS-box repressor genes FLC and MAF4

Ortuño-Miquel

Rodríguez-Cazorla

Zavala-González³

et al. 2019

Sci Rep

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The adaptive success of flowering plants is largely due to their ability to align floral production with optimal conditions. In Arabidopsis thaliana, MADS-box repressors of the FLC/MAF-clade prevent flowering under non-inductive conditions, although the role of some members is not yet clearly defined. Using a genetic strategy, we identified the KH-domain gene HEN4, previously shown to be involved in MADS-box floral homeotic gene regulation, as a modulator of flowering time. Loss-of-function hen4 mutants are early-flowering, and their response to low growth-temperature (16 °C) and day-length is altered. Interestingly, hen4 plants showed dramatic reduction of FLC and MAF4 transcripts, whereas other flowering repressors of the same clade (FLM, MAF2, MAF3, MAF5) remained unaltered. We also determined that hen4, partly due to loss of FLC, accelerates the vegetative phase-change. This report provides insight into flowering time control and highlights the potential of versatile regulators such as HEN4 to coordinate the juvenile-to-adult transition and floral timing.

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Ovule identity mediated by pre-mRNA processing in Arabidopsis

Cited by 18 publications

References 124 publications

Dissection of the Arabidopsis HUA‐PEP gene activity reveals that ovule fate specification requires restriction of the floral A‐function

Dissection of the Arabidopsis HUA‐PEP gene activity reveals that ovule fate specification requires restriction of the floral A‐function

Growth dynamics of the Arabidopsis fruit is mediated by cell expansion

Arabidopsis HUA ENHANCER 4 delays flowering by upregulating the MADS-box repressor genes FLC and MAF4

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