Ovulation, fertilization and early embryonic development in the bitch (Canis familiaris)

Renton, J.P.; Boyd, J. Morton; Eckersall, P. D.; Ferguson, Joyce; Harvey, Michael J.; Mullaney, J.; Perry, B.

doi:10.1530/jrf.0.0930221

Cited by 59 publications

(44 citation statements)

References 7 publications

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“…In our study, the mean number of corpora lutea per bitch was slightly higher than that observed classically (Doak et al 1967, Tsutsui & Shimizu 1975, Shimizu et al 1990, Renton et al 1991, Bysted et al 2001.…”

Section: Discussioncontrasting

confidence: 69%

“…Most studies, in both the fox and the dog, have been conducted on very few females (from six to 25) over a very large period of time (from 0 to 24 days after ovulation) (Doak et al 1967, Tsutsui 1975, Tsutsui & Shimizu 1975, Renton et al 1991, Bysted et al 2001. Furthermore, the timing of maturation and embryonic development has been described with reference to several events more or less timely related to ovulation: onset of oestrus, acceptance of the male, LH peak, aspect of follicles at laparotomy or serum progesterone levels (see for example Tsutsui 1975, 1989, Archbald et al 1980, Renton et al 1991, Bysted et al 2001. No study has been conducted with real visualization of ovulation, which appears to be the best reference for the description of oocyte maturation and embryo development.…”

Section: Discussionmentioning

confidence: 99%

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In vivo meiotic resumption, fertilization and early embryonic development in the bitch

Reynaud¹,

Fontbonne²,

Marseloo³

et al. 2005

Reproduction

110

112

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Early development in canine species follows a very specific pattern. Oocytes are ovulated at the germinal vesicle stage and meiotic resumption occurs in the oviduct. However, because of difficulties in the accurate determination of ovulation time and in the observation of oocyte nuclear stage by light microscopy, these early events have not been fully described. Moreover, the oocyte stage at which sperm penetration occurs is still uncertain since fertilization of immature oocytes has been reported in vivo and in vitro. The aim of this study was to establish the exact timing of in vivo meiotic resumption, fertilization and early embryo development in the bitch with reference to ovulation. Ovulation was first determined by ultrasonography, artificial inseminations were performed daily and oocytes/embryos were collected between 17 and 138 h after ovulation. After fixation and DNA/tubulin staining, the nuclear stage was observed by confocal microscopy. Of the 195 oocytes/embryos collected from 50 bitches, the germinal vesicle stage was the only one present until 44 h post-ovulation, and the first metaphase II stage was observed for the first time at 54 h. Sperm penetration of immature oocytes appeared to be exceptional (three out of 112 immature oocytes). In most cases, fertilization occurred from 90 h post-ovulation in metaphase II oocytes. Embryonic development was observed up to the eight-cell stage. No significant influence of bitch breed and age on ovulation rate, maturation and developmental kinetics was observed. However, some heterogeneity in the maturation/development process was observed within the cohort of oocytes/embryos collected from one bitch. In conclusion, the most peculiar aspect of the canine species remains oocyte meiotic maturation whereas fertilization follows the same pattern as in other mammals. Reproduction (2005) 130 193-201

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Section: Discussioncontrasting

confidence: 69%

Section: Discussionmentioning

confidence: 99%

In vivo meiotic resumption, fertilization and early embryonic development in the bitch

Reynaud¹,

Fontbonne²,

Marseloo³

et al. 2005

Reproduction

110

112

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show abstract

“…Los embriones ingresan al útero 10 a 12 días después de la fecundación, en estado de mórula o blastocisto temprano (Tsutsui, 1989). Blastocistos más avanzados solo se describen en los cuernos uterinos (Renton et al, 1991). Un aspecto particular del desarrollo embrionario canino es que no sigue un patrón bien definido, existiendo acuerdo en cuanto a la asincronía del proceso para embriones producidos en una misma ovulación.…”

Section: Desarrollo Embrionariounclassified

Biología Gestacional y Predicción del Parto en la Perra

Riquelme

Ruiz

2017

Rev. investig. vet. Perú

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RESUMENLa gestación en la perra transcurre entre la fecundación y el parto, y su duración tiene importancia clínica. La compleja biología de los gametos y embriones influye en la duración de la gestación, cuyo lapso fisiológico fluctúa entre los 57 y 70 días. Este amplio rango puede estar influenciado por los métodos referenciales para la estimación del inicio de la gestación. El realizar montas o inseminaciones en base a detección de la ovulación permite estrechar el rango a 62 a 64 días. Una condición de alto impacto obstétrico es la gestación de un solo cachorro, la cual es más prolongada y representa alto riesgo de distocia. Dada la importancia clínica de una adecuada proyección de la duración de la gestación y de la predicción de la fecha de parto, se han desarrollado fórmulas matemá-ticas para el cálculo de la edad fetal y tiempo al parto a través de la sistematización de la ultrasonografía gestacional.Palabras clave: perra; duración de gestación; parto ABSTRACTThe pregnancy in the bitch occurs between fertilization and whelping, and its length has clinical importance. The complex biology of the gametes and embryos influences the length of the gestation, where the physiological period fluctuates from 57 to 70 days. This wide range can be influenced by the referential methods used to estimate the starting day. Controlled natural mating or artificial insemination based on the detection of ovulation allows reducing the range from 62 to 64 days. A high impact obstetric condition is the

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“…Subsequent stages of the meiotic maturation are resumed in the oviduct and take 2-3 days to complete [14]. Moreover, the canine oocyte is very rich in lipids and the cumulus cell mass around the oocyte is tight and multilayered and remains attached to the gamete longer after fertilization [37].…”

Section: In Vitro Embryo Production In Dogsmentioning

confidence: 99%

Current progress on assisted reproduction in dogs and cats: in vitro embryo production

Luvoni

2000

Reprod. Nutr. Dev.

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-The objective of the development of assisted reproduction techniques in dogs and cats is their application to non-domestic canine and feline species, most of which are considered threatened or endangered. Among these techniques, an entirely in vitro system for embryo production is effectively an important tool for conservation of wildlife. In the last decade, progress has been made in embryo production in carnivores. It has been shown that canine oocytes can resume meiosis in vitro and that these oocytes can be fertilized and developed in vitro, although at a much lower rate than most other domestic animal oocytes. The reason lies in the dissimilarities of reproductive physiology of the dog compared to other species and the lack of precise information concerning the oviductal environment, in which oocyte maturation, fertilization and early embryonic development take place. Successful in vitro embryo production in the domestic cat has been attained with oocytes matured in vitro, and kittens were born after transfer of IVM/IVF derived embryos. On the basis of these results the in vitro fertilization of oocytes has also been applied in several non-domestic feline species. The effectiveness of such protocols in the preservation of genetic material of rare species can be improved by developing better techniques for long-term storage of gametes. In dogs and cats sperm cells have been successfully frozen and the cryopreservation of oocytes would greatly increase their availability for a range of reproductive technologies. Cryopreserved cat oocytes can be fertilized successfully and their development in vitro after fertilization is enhanced when mature oocytes are frozen. Thus refined techniques of oocyte maturation and fertilization in vitro coupled with oocyte cryopreservation could allow for an easy establishment of genetic combinations when male and female gametes in the desired combination are not simultaneously available, and the propagation of endangered carnivores would be facilitated.canine / feline / in vitro embryo production Résumé -Progrès récents de la reproduction assistée des chiens et des chats : production in vitro d'embryons. L'objectif du développement des techniques de reproduction assistée chez le chien et le chat ont leur application à des espèces canines et félines non domestiques, la plupart d'entre elles menacées ou en danger. En effet un système de production d'embryons entièrement Reprod. Nutr. Dev. 40 (2000) 505-512 505

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Ovulation, fertilization and early embryonic development in the bitch (Canis familiaris)

Cited by 59 publications

References 7 publications

In vivo meiotic resumption, fertilization and early embryonic development in the bitch

In vivo meiotic resumption, fertilization and early embryonic development in the bitch

Biología Gestacional y Predicción del Parto en la Perra

Current progress on assisted reproduction in dogs and cats: in vitro embryo production

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