Ovulation as an Inflammatory Reaction—A Hypothesis

Espey, Lawrence L.

doi:10.1095/biolreprod22.1.73

Cited by 743 publications

(334 citation statements)

References 0 publications

Supporting

Mentioning

312

Contrasting

Unclassified

Order By: Relevance

“…NO produced by increasing NOS after hCG injection may contribute to this change of local circulation. Numerous factors are involved in inducing follicular rupture [40]. For example, progesterone, which increases as a result of the LH surge and peaks at 10 h after hCG injection [13], is one of the important factors in ovulation [12]; in fact, progesterone receptor knockout mice fail to ovulate [4i].…”

Section: Discussionmentioning

confidence: 99%

Changes in Nitric Oxide Synthase Activity in the Ovary of Gonadotropin Treated Rats. The Role of Nitric Oxide during Ovulation.

et al. 1999

View full text Add to dashboard Cite

Abstract.Immature rats receiving equine chorionic gonadotropin (eCG) and human CG (hCG) were used to study the time course changes in nitric oxide synthase (NOS) activity in the ovary during ovulation.To study the role of NO in ovulation, the effects of intrabursal injection of L-NG-monomethylarginine (L-NMMA, 125 pg/20 pl/bursa), a NOS inhibitor, on the number of ova shed were also examined.Rats were sacrificed at -48, 0, 3, 6, 9, 12, and 24 h after hCG injection, and the ovaries were collected for the NOS activity assay, Western blotting, NADPH-diaphorase histochemistry and immunohistochemistry.Total NOS and constitutive NOS activities in the ovary increased significantly at 9 h after hCG injection and the values remained high thereafter.Inducible NOS (iNOS) activity was detectable as a small peak at 3 and 6 h after hCG injection. Endothelial NOS (eNOS) protein production increased after hCG injection with a peak at 12 h, whereas iNOS protein production decreased at 12 and 24 h after hCG injection. NADPH-diaphorase positive cells increased at the thecae of growing follicles after hCG injection, appeared at mural granulosa cells before ovulation, and were detected in newly formed corpora lutea, which coincided with the results in eNOS positive cells by immunohistochemistry.L-NMMA given to rats at 5 or 7 h after hCG was most effective in reducing the number of ova shed. These results indicate that the NOS activity and NOS positive cells increased after hCG injection, and that eNOS was likely the main NOS increasing in the ovary during ovulation. It is concluded that NO produced between 5 and 9 h after hCG might play a supportive role in ovulation.

show abstract

Section: Discussionmentioning

confidence: 99%

Changes in Nitric Oxide Synthase Activity in the Ovary of Gonadotropin Treated Rats. The Role of Nitric Oxide during Ovulation.

et al. 1999

View full text Add to dashboard Cite

show abstract

“…The ovulatory process could be considered as an inflammatory reaction [7], and leukocytes, especially neutrophils and monocytes/macrophages, have been shown to infiltrate the ovaries just before ovulation [2,5,8]. It has been suggested that IL-1β derived from infiltrating leukocytes enhanced ovulation [3,10,20,21].…”

Section: Discussionmentioning

confidence: 99%

Detection of Interleukin-1.BETA. in Sera and Colostrum of Dairy Cattle and in Sera of Neonates.

Goto

Maruyama

Kitadate

et al. 1997

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

ABSTRACT. In order to obtain basic information about bovine interleukin-1 (IL-1β), levels of IL-1β in sera and milk of clinically normal mature Holstein cattle before and after parturition and in sera of newborn calves were examined by ELISA. The level of IL-1β was undetectable in sera of mature cattle around the time of artificial insemination, but the concentration gradually increased and reached a peak at parturition and then decreased again to an undetectable level. IL-1β in milk was detected on the day of parturition but not thereafter. IL-1β mRNA was detected by reverse transcription-polymerase chain reaction in the cells from milk collected during 20 days before and 2 to 3 days after parturition, but was not detected thereafter. Although IL-1β was not detected in all the sera of newborn calves, the concentration transiently increased with peak titers on day 3 and became undetectable by day 14 after birth. Newborns that showed serum IL-1β on day 3 had been fed on colostrum in which the IL-1β concentration was significantly higher than that in colostrum that had been fed to newborns having no detectable IL-1β on day 3. These results indicate that IL-1β is induced in association with pregnancy in healthy dairy cattle and that the cytokine might be transferred to neonates via colostrum. -KEY WORDS: cattle, colostrum, interleukin-1, pregnancy.

show abstract

“…The present results demonstrate that the macrophage is one of the anti-TGF/3 positive cells in functional luteal tissue. It is known that macrophages migrate into the ruptured follicle after ovulation and reside in newly formed luteal tissue [27,49]. It is inferred that these macrophages play a role in regulating luteal function by secreting cytokines [28,30,35,36,[50][51][52][53][54][55][56].…”

Section: Discussionmentioning

confidence: 99%

“…In fact, it is reported that large luteal cells and small luteal cells in rat corpus luteum were stained with the anti-TGF/31 and anti-TGF/32, respectively [24]. TGF/3 is one of the cytokines secreted from macrophages [2,33], and macrophages are known to be present in corpora lutea [25][26][27][28][29][30][31][32]. The luteal macrophage is therefore also a possible candidate for the anti-TGF/3 positive cell in the corpus luteum.…”

Section: Transformingmentioning

confidence: 99%

Immunoreactive (ir)-Transforming Growth Factor (TGF)-.BETA. in Rat Corpus Luteum: ir-TGF.BETA. Is Expressed by Luteal Macrophages.

Matsuyama

Takahashi

1995

Endocr J

View full text Add to dashboard Cite

Abstract. Western analyses and immunohistochemistryof transforming growth factor /31(TGF/31) and TGF/32 were performed in rat luteal tissue at either the functional or regressing stage. Anti-TGF/31 or TGF/32 was localized simultaneously to macrophages within cultured luteal cells and frozen ovarian sections. By Western analysis, an active form of TGF/3 (25 kDa) was detected clearly with anti-TGFf32 but only faintly with anti-TGF/31, and the former band from functional corpora lutea was more intense than that from regressing ones. Treatment with prolactin (PRL), a luteotropic hormone in rodents, also increased the intensity of 25 kDa TGF/32 in the corpus Luteum. Several specific bands with higher molecular weights than 25 kDa were also recognized with anti-TGF/31 or TGFI32; they are probably ascribed to latent TGFJ3s and/or TGF/3 precursor proteins. In cultured luteal cells and frozen ovarian sections, many anti-TGF/31 or anti-TGF/32 positive cells from functional corpora lutea of pseudopregnant rats were double-stained with anti-macrophage. There were numerous macrophages in structurally regressing corpora lutea of pseudopregnant rats, but most of them were not stained with anti-TGF/3s. These results suggest that the expression of TGF/3, at least for TGF/32, is under the influence of PRL in the rat corpora lutea, and that macrophages are responsible for some, if not all, of the Immunoreactive-TGF/3 in rat luteal tissue.

show abstract

Ovulation as an Inflammatory Reaction—A Hypothesis

Cited by 743 publications

References 0 publications

Changes in Nitric Oxide Synthase Activity in the Ovary of Gonadotropin Treated Rats. The Role of Nitric Oxide during Ovulation.

Changes in Nitric Oxide Synthase Activity in the Ovary of Gonadotropin Treated Rats. The Role of Nitric Oxide during Ovulation.

Detection of Interleukin-1.BETA. in Sera and Colostrum of Dairy Cattle and in Sera of Neonates.

Immunoreactive (ir)-Transforming Growth Factor (TGF)-.BETA. in Rat Corpus Luteum: ir-TGF.BETA. Is Expressed by Luteal Macrophages.

Contact Info

Product

Resources

About