Ovulation after sympathetic denervation of the rat ovary produced by freezing its nerve supply

Wylie, S. N.; Roche, P. J.; Gibson, W. P. R.

doi:10.1530/jrf.0.0750369

Cited by 17 publications

(10 citation statements)

References 12 publications

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“…In earlier studies we showed that brief freezing of the ovarian nerve supply produces complete adrenergic denervation of the ovary and the oviduct (Gibson, Roche, Summers & Wylie, 1984) but does not interfere with ovulation (Wylie, Roche & Gibson, 1985). In the present study we extend this work to test whether pregnancy can be initiated and maintained and whether normal parturi¬ tion can occur when the adrenergic innervation of the ovary, oviduct, mesosalpinx, utero-tubal junction and upper uterine horn is eliminated.…”

Section: Introductionsupporting

confidence: 54%

Pregnancy and parturition in rats after sympathetic denervation of the ovary, oviduct and utero-tubal junction

Roche¹,

Parkington²,

Gibson³

1985

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The ovarian vascular pedicle and ovarian suspensory ligament were briefly frozen to destroy the nerves. Examination of sections from the ovary, oviduct and utero-tubal junction by fluorescence histochemistry showed that they were usually devoid of adrenergic nerves. Measurement of noradrenaline in segments of the uterine horn by high-performance liquid chromatography showed that the transmitter was eliminated from the upper third but not the middle or lower thirds of the uterine horn. Unilateral or bilateral denervations at metoestrus in cyclic rats did not affect either the number of ovulations or the numbers or spacing of conceptuses at Day 7 of pregnancy. Bilateral denervations on Days 4, 7 or 11 of pregnancy did not affect ovarian weights or numbers of conceptuses observed 1 week later. Plasma progesterone concentrations were at least as high in the denervated groups as in the sham-operated control groups. After unilateral or bilateral denervations at Day 15, pregnancy continued normally and birth of normal young occurred, without apparent problems, at the same time as for sham-operated rats. The mothers tended their young and allowed them to suck. It is concluded that the adrenergic innervation of the ovary in the rat is not required for its normal function during pregnancy; that of the oviduct and mesosalpinx is not required for ovum pick-up or for transport of oocytes, spermatozoa or early embryos; and that of the utero-tubal junction is not required for uterine motility involved in embryo spacing and parturition.

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Section: Introductionsupporting

confidence: 54%

Pregnancy and parturition in rats after sympathetic denervation of the ovary, oviduct and utero-tubal junction

Roche¹,

Parkington²,

Gibson³

1985

Reproduction

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“…The relation between ovulation and ovarian innervation has also been discussed (Bahr et al, 1974) and it has been suggested that there is no direct relation between them (Wylie et al, 1985). However, the intrabursal application of norepinephrine and adrenergic agonists and antagonists in the ovary showed a significant increase in the number of new corpora lutea in prepuberal rats primed with PMSG (Kannisto et al, 1985).…”

Section: Innervation and Ovarian Functionmentioning

confidence: 99%

Role of the central and peripheral nervous system in the ovarian function

Aguado

2002

Microscopy Res & Technique

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This review attempts to give a comprehensive overview of ovarian innervation, considering the whole nervous system and its different levels that may modify the ovarian function. The connection between the ovary and the central nervous system through the autonomic pathways, including the peripheral ganglia, is highlighted. The evidence obtained over the last years highlights the role of the superior ovarian nerve (SON) in the ovarian phenomena. Besides, the effect on the ovary of conventional neurotransmitters and others such as indolamines and peptides, which have been found in this organ, are discussed. Various reproductive diseases have been studied almost exclusively from the endocrine point of view. It is evident that a better knowledge about the role of the neural factors involved in the ovarian physiology may facilitate the understanding of some of these. A review of the concepts and an update of some experimental designs is made that permits clarifying several aspects of the relationship between the neural system and the ovary. At present, there is no doubt that the innervation of the ovary is involved in several physiological aspects of this gland function. However, the relationship of some levels of the nervous system and the ovary offer a wide avenue for future research.

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“…There are numerous reviews and articles on sperm transport in the female genital tract [16,27,28] and the main objective with the transport is obviously to establish a population of fertilizable or viable spermatozoa near the oocyte prior to the process of ovulation by inducing contraction of the follicular wall prior to the collapse of the preovulatory follicle [43]. Other studies have, however, shown that denervation of the ovary has no adverse effect on the ovulatory process so the effect of the nervous system is still not clear [58]. The duration of the ovulatory process is estimated to be approximately 6 min in the mare [55], 1.8 h in pigs [50] and 4-12 h in the superovulated cow [3,4].…”

Section: The Spermatozoamentioning

confidence: 99%

Rendez-vous in the oviduct: implications for superovulation and embryo transfer

Greve

Callesen²

2001

Reprod. Nutr. Dev.

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-The meeting between the maternal and paternal gametes is dependant upon a number of complicated processes. On the maternal side it involves maturation of the oocytes under the influence on both peripheral and follicular endocrine factors. Deviations in the normal pattern of maturation will lead to ovulation of inferior oocytes. On the paternal side the transport of spermatozoa in the female genital tract following mating is an area of great importance. The establishment of the sperm reservoir in the isthmus is dependant upon a number of factors (intracellular calcium concentrations, oligo-saccharides, change in estradiol: progesterone ratio in the afferent blood supply). Alterations of the normal micro-environment may disturb both binding, release and transport as a whole. The process of fertilization occurs in the ampullar region of the oviduct and it involves several well tuned steps: binding to the zona pellucida where the acrosome reaction takes place, penetration, fusion between the oolemma and the sperm plasma membrane, activation with the release of the cortical granules, decondensation of the sperm chromatin, pronucleus formation and finally syngamy where the two pronuclei fuses. The egg will experience the first cleavage shortly thereafter. Superovulation may disturb a number of these processes including oocyte maturation (arrest at MI) and sperm and zygote transport in the oviduct caused by the deviant endocrine environment, thus leading to a higher incidence of lack of fertilization and poor embryos quality. oocyte maturation / capacitation / fertilization / superovulation Reprod. Nutr. Dev. 41 (2001) [451][452][453][454][455][456][457][458][459] 451

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Ovulation after sympathetic denervation of the rat ovary produced by freezing its nerve supply

Cited by 17 publications

References 12 publications

Pregnancy and parturition in rats after sympathetic denervation of the ovary, oviduct and utero-tubal junction

Pregnancy and parturition in rats after sympathetic denervation of the ovary, oviduct and utero-tubal junction

Role of the central and peripheral nervous system in the ovarian function

Rendez-vous in the oviduct: implications for superovulation and embryo transfer

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