Ovocalyxin-36 and other LBP/BPI/PLUNC-like proteins as molecular actors of the mechanisms of the avian egg natural defences

Gautron, Joël; Réhault-Godbert, Sophie; Pascal, Géraldine; Nys, Yves; Hincke, Maxwell T.

doi:10.1042/bst0390971

Cited by 39 publications

(28 citation statements)

References 42 publications

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“…They are very effective barriers to bacterial penetration and at the same time have antibacterial properties (Gautron et al, 2001a;Gautron et al, 2001b;Gautron et al, 2011). The shell membranes are built up of three distinct layers: (i) the inner shell membrane (ISM; lies immediately over the albumen); (ii) the outer shell membrane (OSM; attached to the eggshell), which consists of a network of randomly oriented fibres; and (iii) a homogeneous third layer of electron-dense material called the limiting membrane (Bruce and Drysdale, 1994).…”

Section: The Shell Membranesmentioning

confidence: 99%

“…These fibres have a complement of antibacterial proteins (e.g. ovotransferrin, lysozyme, ovocalyxin) (Hincke et al, 2000;Gautron et al, 2001a;Gautron et al, 2001b;Gautron et al, 2011). The inner and outer shell membranes are attached at all points except at the blunt end of the eggshell where they separate and the air cell is formed.…”

Section: The Shell Membranesmentioning

confidence: 99%

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Scientific Opinion on the public health risks of table eggs due to deterioration and development of pathogens

2014

EFSA Journal

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Salmonella Enteritidis is considered the only pathogen currently posing a major risk of egg-borne diseases in the European Union (EU). The possible impact of extending the shelf-life of eggs on the risk to consumers posed by S. Enteritidis was estimated by applying a quantitative model and comparing the actual situation regarding the storage of eggs in the EU with different possible scenarios combined, considering the prolongation of the bestbefore and the sell-by date from 7 to a maximum of 70 days. Extending the sell-by date by one week (from 21 to 28 days), but leaving the best-before date unchanged, is estimated to result in a relative risk of illness of 1.4 and 1.5 for uncooked and lightly cooked egg meals respectively, compared to the current situation. If the best-before date is also extended by one week (from 28 to 35 days), the relative risk would be 1.6 and 1.7. In the worst case scenario considered (sell-by date of 42 days, best before date of 70 days), such figures would be 2.9 and 3.5. It should be noted that the absolute risk is greater for uncooked meals compared to lightly cooked meals. An effective way to minimise any increase in risk during extended storage is to keep the eggs refrigerated both at retail and the household. Regarding egg spoilage, such events strongly depend on the hygienic conditions of egg production and practices of egg handling, including storage times and temperatures. Finally, the impact of the prolongation of storage time on the quality criteria for eggs (3-hydroxybutyric acid and lactic acid) destined for manufacturing of egg products is considered negligible. Specifically, EFSA was asked to assess: (i) the public health risk posed by relevant pathogens and in particular by Salmonella in the consumption and handling of table eggs (Term of Reference, or ToR1), (ii) the public health risk deriving from deterioration (ToR2) and (iii) possible consequences for public health of an extended shelf-life of table eggs for the specific freshness criteria for egg products as laid out in the hygiene package (ToR3). © European Food Safety Authority, 2014 KEY WORDSIn order to answer the first ToR, the BIOHAZ Panel based its conclusions on the results of a quantitative model, aimed at describing the behaviour of Salmonella Enteritidis following vertical transmission, since this serovar is recognised to be the major pathogen related to egg-borne disease because of its ability to contaminate the interior of intact eggs during their formation within the body of an infected hen. The possible impact of extending the shelf-life of eggs on other serovars of Salmonella, as well as on other pathogens, is evaluated in a qualitative manner, with no individual serovar other than S. Enteritidis currently posing a major risk of egg-borne salmonellosis in the European Union (EU), although S. Typhimurium has been associated with relatively small outbreaks due to duck eggs. Trans-shell contamination (i.e. secondary contamination) is also discussed, but the impact of a prolongation of the shelf ...

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Section: The Shell Membranesmentioning

confidence: 99%

Section: The Shell Membranesmentioning

confidence: 99%

Scientific Opinion on the public health risks of table eggs due to deterioration and development of pathogens

2014

EFSA Journal

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“…OCX-36 is the most abundant in the basal shell and associated membranes. Sequence comparison analysis reveals that OCX-36 is related to the antibacterial protein superfamily BPI/LBP/PLUNC and shares 20-27% identity with BPI/LBP/PLUNC members (Gautron et al , 2007; Gautron et al , 2011b). Additionally, OCX-36 is a pattern recognition molecule, which recognizes bacterial endotoxins as the first step to eliminate pathogens, and it also plays an important role during calcification (Cordeiro et al , 2013; Gautron et al , 1997; Hincke et al , 2010; Kovacs-Nolan et al , 2014).…”

Section: Introductionmentioning

confidence: 99%

An Efficient Method for Co-purification of Eggshell Matrix Proteins OC-17, OC-116, and OCX-36

Zhang¹,

Wang²,

Xu³

et al. 2016

Korean Journal for Food Science of Animal Resources

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In this study, we improved the eggshell-membrane separation process by separating the shell and membrane with EDTA solution, evaluating effects of three different extraction solutions (acetic acid, EDTA, and phosphate solution), and co-purifying multiple eggshell proteins with two successive ion-exchange chromatography procedures (CM Sepharose Fast Flow and DEAE Sepharose Fast Flow). The recovery and residual rates of eggshell and membrane separated by the modified method with added EDTA solution were 93.88%, 91.15% and 1.01%, 2.87%, respectively. Ovocleidin-116 (OC-116) and ovocalyxin-36 (OCX-36) were obtained by loading 50 mM NaHepes, pH 7.5, 2 mM DTT and 350 mM NaCl buffer onto the DEAE-FF column at a flow rate of 1 mL/min, ovocleidin-17 (OC-17) was obtained by loading 100 mM NaCl, 50 mM Tris, pH 8.0 on the CM-FF column at a flow rate of 0.5 mL/min. The purities of OCX-36, OC-17 and OC-116 were 96.82%, 80.15% and 73.22%, and the recovery rates were 55.27%, 53.38% and 36.34%, respectively. Antibacterial activity test suggested that phosphate solution extract exhibited significantly higher activity against the tested bacterial strains than the acetic acid or EDTA extract, probably due to more types of proteins in the extract. These results demonstrate that this separation method is feasible and efficient.

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“…The following proteins have been examined: ovalbumin, a protein that is synthesized mainly in the magnum and that constitutes 54 % of the egg white (Nys et al 2001), and the recently described eggshell-matrix proteins produced predominantly in the shell gland (Gautron et al 2001, 2007; Hincke et al 2003), i.e. ovocalyxin-32, which influences the variability of crystal traits and, in turn, the thickness profile of the shell (Takahashi et al 2010; Dunn et al 2012; Fulton et al 2012) and ovocalyxin-36, whose expression is strongly upregulated during shell calcification and which has antimicrobial activity and might play a role in the natural defence of the egg against pathogens (Gautron et al 2011). …”

Section: Introductionmentioning

confidence: 99%

Chicken oviduct—the target tissue for growth hormone action: effect on cell proliferation and apoptosis and on the gene expression of some oviduct-specific proteins

et al. 2014

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The aim of this study was to examine the in vivo effect of growth hormone (GH) on cell proliferation and apoptosis and on the gene expression of selected proteins in the chicken oviduct before sexual maturity (first oviposition). Ten-week-old Hy-Line Brown chickens were injected three times a week with 200 μg · kg-1 body weight of recombinant chicken GH (cGH) until 16 weeks of age. Control hens received 0.9 % NaCl with 0.05 % bovine serum albumin as a vehicle. Treatment with cGH increased (P < 0.05) oviduct weight at 16 weeks of age, i.e. 1–2 weeks before onset of egg laying. The highest number of proliferating (determined by proliferating cell nuclear antigen [PCNA] immunocytochemistry) and apoptotic (determined by TUNEL assay) cells in the oviduct was found in the mucosal epithelium, and the lowest in the stroma. Administration of cGH did not increase (P > 0.05) the number of PCNA-positive cells but it decreased (P < 0.01) the number of TUNEL-positive cells, thus increasing the proliferating-to-apoptotic cell ratio in the oviduct. Gene expression (determined by real-time polymerase chain reaction) of apoptosis-related caspase-2 in the magnum and caspase-3 in the magnum and isthmus and their activity (determined by fluorometric assay) in the magnum were attenuated (P < 0.05) in cGH-treated hens. The gene expression of the magnum-specific ovalbumin and the shell-gland-specific ovocalyxins 32 and 36 was increased (P < 0.05) in cGH-treated chickens. In contrast, the expression of Bcl-2 and of caspases 8 and 9 was not affected by cGH in any of the oviductal segments. The results suggest that GH, via the orchestration of apoptosis and expression of some oviduct-specific proteins, participates in the development and activity of the chicken oviduct prior to the onset of egg laying.

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Ovocalyxin-36 and other LBP/BPI/PLUNC-like proteins as molecular actors of the mechanisms of the avian egg natural defences

Cited by 39 publications

References 42 publications

Scientific Opinion on the public health risks of table eggs due to deterioration and development of pathogens

Scientific Opinion on the public health risks of table eggs due to deterioration and development of pathogens

An Efficient Method for Co-purification of Eggshell Matrix Proteins OC-17, OC-116, and OCX-36

Chicken oviduct—the target tissue for growth hormone action: effect on cell proliferation and apoptosis and on the gene expression of some oviduct-specific proteins

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