Oviposition-deterrent, ovicidal, and repellent activities of indigenous plant extracts against Anopheles subpictus Grassi (Diptera: Culicidae)

Abstract

Insecticides of botanical origin may serve as suitable alternative biocontrol techniques in the future. The leaf acetone, ethyl acetate, and methanol extracts of Aegle marmelos (Linn.) Correa ex Roxb, Andrographis lineata Wallich ex Nees, and Cocculus hirsutus (L.) Diels were tested for oviposition-deterrent, ovicidal, and repellent activities against Anopheles subpictus Grassi (Diptera: Culicidae). The percentage of effective oviposition repellency of 92.60 , 93.04, 95.20, 88.26, 92.80, 94.01, 95.77, 96.93, and 92.54 at 500 ppm and the lowest repellency of 47.14, 58.00, 56.52, 64.93, 71.09, 66.42, 50.62, 57.62, and 65.73 at 31.25 ppm in acetone, ethyl acetate, and methanol extracts of Aegle marmelos, Andrographis lineata, and Cocculus hirsutus, respectively. The oviposition activity index (OAI) value of acetone, ethyl acetate, and methanol extracts of Aegle marmelos, Andrographis lineata, and Cocculus hirsutus at 500 ppm were -0.86, -0.87, -0.90, -0.78, -0.87, -0.86, -0.91, -0.94, and -0.86 respectively. The OAI values revealed that the solvent plant extracts have deterrent effect, and they caused a remarkable negative response resulting in oviposition of very few eggs. Mean percent hatchability of the ovicidal activity was observed 24 h after treatment. The percent hatchability was inversely proportional to the concentration of extract and directly proportional to the eggs. Mortality of 100% with ethyl acetate extract of Aegle marmelos, methanol extracts Aegle marmelos, Andrographis lineata, and Cocculus hirsutus were exerted at 1,000 ppm. The maximum repellent activity was observed at 500 ppm in methanol extracts of Aegle marmelos, Andrographis lineata, and ethyl acetate extract of Cocculus hirsutus, and the mean complete protection time ranged from 90 to 120 min with the different extracts tested. These results suggest that the leaf extracts of Aegle marmelos, Andrographis lineata, and Cocculus hirsutus have the potential to be used as an ideal ecofriendly approach for the control of the Anopheles subpictus. Therefore, this study provides first report on the oviposition, ovicidal, and repellent activities against malaria vector, Anopheles subpictus of plant extracts from Southern India.

Repellent properties of Cardiospermum halicacabum Linn. (Family: Sapindaceae) plant leaf extracts against three important vector mosquitoes

“…aegypti mosquitoes. The results are comparable with an earlier report by Elango et al [30] that the maximum repellent activity was observed at 500 ppm in methanol extracts of Aegle marmelos, Acacia lineata, and ethyl acetate extract of Chamaecytisus hirsutus, and the mean complete protection time ranged from 90 to 120 min against Anopheles subpictus. The hexane extract of Andrographis paniculata was more effective in exhibiting the repellent action against the mosquito as compared with Acacia lineate extract and the complete protections was observed for 150 min in hexane extract of Andrographis paniculata at 500 ppm against Culex tritaeniorhynchus bites [31] .…”

Mosquito repellent potential of Pithecellobium dulce leaf and seed against malaria vector Anopheles stephensi (Diptera: Culicidae)

“…stephensi. respectively [18]. In conclusion, the present study clearly proved that the efficacy of leaf and seed extracts of P. dulce can be suggested as a repellent agent against An.…”

Chemosensory Cues for Mosquito Oviposition Site Selection

“…Not surprisingly, these plants were found to be deterrent for mosquito oviposition , Elango et al 2009, Rajkumar and Jebane san 2009, Warikoo et al 2011, showing that mosqui toes avoid conditions that are noxious to their offspring. However, suitability for the offspring does not always explain the oviposition effect of plant infusions; the water soluble lectin isolated from the Moringa oleifera Lamarck tree is larvicidal and ovicidal against Ae.…”

“…Other plants (such as Solenostemma argel Delile) have a negative effect on mosquito eggs , Elango et al 2009, Warikoo et al 2011 or larvae (Al Doghairi et al 2004, Rajkumar andJebane san 2009). Not surprisingly, these plants were found to be deterrent for mosquito oviposition , Elango et al 2009, Rajkumar and Jebane san 2009, Warikoo et al 2011, showing that mosqui toes avoid conditions that are noxious to their offspring.…”