Oviposition Deterrent and Larvicidal and Pupaecidal Activity of Seven Essential Oils and their Major Components against Culex quinquefasciatus Say (Diptera: Culicidae): Synergism–antagonism Effects

Abstract

Abstract:The larvicidal activity of essential oils cinnamon (Cinnamomum verum J. Presl), Mexican lime (Citrus aurantifolia Swingle) cumin (Cuminum cyminum Linnaeus), clove (Syzygium aromaticum (L.) Merr. & L.M.Perry), laurel (Laurus nobilis Linnaeus), Mexican oregano (Lippia berlandieri Schauer) and anise (Pimpinella anisum Linnaeus)) and their major components are tested against larvae and pupae of Culex quinquefasciatus Say. Third instar larvae and pupae are used for determination of lethality and mortality. Essential oils with more than 90% mortality after a 30-min treatment are evaluated at different time intervals. Of the essential oils tested, anise and Mexican oregano are effective against larvae, with a median lethal concentration (LC 50 ) of 4.7 and 6.5 µg/mL, respectively. Anise essential oil and t-anethole are effective against pupae, with LC 50 values of 102 and 48.7 µg/mL, respectively. Oregano essential oil and carvacrol also have relevant activities. A kinetic analysis of the larvicidal activity, the oviposition deterrent effect and assays of the effects of the binary mixtures of chemical components are undertaken. Results show that anethole has synergistic effects with other constituents. This same effect is observed for carvacrol and thymol. Limonene shows antagonistic effect with β-pinene. The high larvicidal and pupaecidal activities of essential oils and its components demonstrate that they can be potential substitutes for chemical compounds used in mosquitoes control programs.

In vitro and in silico studies of terpenes, terpenoids and related compounds with larvicidal and pupaecidal activity against Culex quinquefasciatus Say (Diptera: Culicidae)

“…Groups of 50 individuals of first and second instar larvae were placed in glass bottles with purified water, maintained at 26 ± 2° C with a natural photoperiod and supplied with 3:1 powdered mixture of dog food and baking powder. The third instar emerging larvae were then separated by groups of 10 individuals in 100 mL tubes with distilled water [ 22 ].…”

Evaluation of the Anti-Trypanosomal Activity of Vietnamese Essential Oils, with Emphasis on Curcuma longa L. and Its Components

“…However these EOs showed a moderate effect in this study with IC 50 values in the range of 8–16 nL/mL. On the contrary, antagonistic effects may also occur between constituents of these mixtures [48]. An interesting example is terpinolene, which accounts for 55% of our C. indica EO (data not shown).…”

“…Major compounds can be studied easier and are often considered as responsible for EO biological activities. However other constituents could of course contribute to the activity through a higher efficacy, additional and/or synergistic activity [48]. For example, eucalyptol, which was characterized as the major component (accounts from 27% to 55%) in four of our tested EOs: A. aromaticum , E. blanda , H. coronarium , and L. cubeba (leaves) (data not shown), was not effective on Tbb as shown by its high IC 50 value (83.15 µg/mL) [49].…”

Essential Oils of Zingiber Species from Vietnam: Chemical Compositions and Biological Activities

“…albopictus (LC 50 of 47.33 and 42.39 μg/mL for (+)-β-pinene and (−)-β-pinene, respectively) [ 53 ] and Cx. quinquefasciatus (LC 50 = 19.6 μg/mL) [ 54 ]. α-Pinene and sabinene have also shown larvicidal activity against Ae.…”