The study was carried out to determine the seroprevalence of Toxoplasmosis in sheep in Babylon province by use of two methods (latex agglutination test (LAT) and Enzyme-linked immunosorbent assay (ELISA)). The results of Latex agglutination have appeared infection rate of 13.77% (31 out of 225 animals), the higher seropositivity was in urban region 19.4% while in rural region it was 11.39%, and depending on sex of animal thus higher rate in males was 16.51% compared with females 11.2%, so the results were referred to a higher rate in young ages (less than one year) were 22.41%, and higher titer for latex agglutination test was 64 with rate 35.48%. With respect ELISA test where the results referred to that out of 31 positive samples for LAT, nine samples were positive for IgG 29.03%.