Ovine subclinical mastitis: Proteomic analysis of whey and milk fat globules unveils putative diagnostic biomarkers in milk

Chiaradia, Elisabetta; Valiani, Andrea; Tartaglia, Micaela; Scoppetta, Fausto; Renzone, Giovanni; Arena, Simona; Avellini, Luca; Benda, S.; Gaiti, Alberto; Scaloni, Andrea

doi:10.1016/j.jprot.2013.03.017

Cited by 34 publications

(35 citation statements)

References 70 publications

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“…Initial studies have already been published about the proteome of ewes' milk (Pisanu et al, 2011;Anagnostopoulos et al, 2014). More recently, biomarkers potentially suitable for early diagnosis of intramammary infections caused by S. chromogenes in ewes have been reported (Chiaradia et al, 2013); increased levels of proteins (SPA, SPB, C3, Ig1g, IgmC, IgLl, ALB, TTR) were observed in the secretion of infected mammary glands. The identification of molecules suitable for the early and accurate diagnosis of intramammary infections can be a fruitful area of future research.…”

Section: Diagnosismentioning

confidence: 99%

Mastitis in sheep – The last 10 years and the future of research

Gelasakis

Mavrogianni

Petridis

et al. 2015

Veterinary Microbiology

144

126

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Section: Diagnosismentioning

confidence: 99%

Mastitis in sheep – The last 10 years and the future of research

Gelasakis

Mavrogianni

Petridis

et al. 2015

Veterinary Microbiology

144

126

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“…Studies related to proteomic analysis of sheep milk confirm that the increase of SCC is not always associated with proteins that characterize infection. Stressful conditions of cellular differentiation, problems in the glandular metabolism possibly related to hormonal changes, and even previous infections may be related to the increase of SCC (CHIARADIA et al, 2013). Interestingly, high SCC levels without microbiological isolation occurred in all breeds, except the Santa Inês sheep.…”

Section: Resultsmentioning

confidence: 95%

Somatic cell count in the diagnosis of subclinical mastitis in sheep of different breeds

Zafalon¹,

Santana²,

Esteves³

et al. 2018

SCA

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The aims of this study were to determine the occurrence of subclinical mastitis in sheep of different breeds and the values for somatic cell count (SCC) in milk for the diagnosis of the disease at lactation and weaning, a fundamental prerequisite for identifying animals in need of control measures. Milk samples were obtained from 1,457 mammary halves of Santa Inês, Texel, Ile de France, and Dorper sheep at two different periods, during the second week of lactation and at weaning. After teats antisepsis, the samples were collected, and identification of the infectious etiology of mastitis and determination of SCC were performed. Microorganisms were identified in 117/762 (15.3%) mammary halves in the second week of lactation and in 86/694 (12.4%) at weaning. Coagulase-negative staphylococci (CoNS) were the etiological agents with the highest incidence alone and in association with other microorganisms, with percentages of 58.1% and 60.6%, respectively. The Santa Inês presented a higher incidence of subclinical mastitis when compared to the other breeds. The cut-off values of SCC for subclinical mastitis were determined at both sampling periods and varied according to stage of lactation, as well breed. These results illustrate the lack of a universal value that can be used for the diagnosis of mastitis and suggests the need for permanent follow-up in herds in order to control the disease. Key words: Diagnostic tests. Milk. Sheep. ResumoOs objetivos do trabalho foram estabelecer a ocorrência da mastite subclínica em ovelhas de diferentes raças e os respectivos valores de triagem da contagem de células somáticas (CCS) no leite para o diagnóstico da doença durante a lactação e ao desmame, um pré-requisito fundamental para a identificação dos animais para o estabelecimento de medidas de controle. As amostras de leite foram obtidas de 1.457 metades mamárias de ovelhas das raças Santa Inês, Texel, Ile de France e Dorper, em dois diferentes períodos, durante a segunda semana de lactação e ao desmame. Após a antissepsia dos tetos, as amostras de leite foram colhidas para identificação da etiologia infecciosa dos casos de mastite e determinação da CCS. Dentre as metades mamárias investigadas, 117/763 (15,3%) apresentaram micro-organismos no leite na segunda semana de lactação e 86/694 (12,4%) apresentaram resultados microbiológicos positivos ao desmame. Estafilococos coagulase-negativos (ECN) foram os agentes etiológicos com maior ocorrência isoladamente e em associação com outros micro-organismos, 58,1% e 60,6%, respectivamente. A raça Santa Inês apresentou maior ocorrência de mastite subclínica, quando comparada às outras raças. Diferentes pontos de corte para CCS foram determinados em ambos os períodos. Os valores das contagens celulares para a triagem da mastite subclínica nas ovelhas variaram de acordo com a fase da lactação em que as amostras foram obtidas, assim como com as raças dos animais, o que denota não existir um valor universal que possa ser usado para o diagnóstico da mastite, sugerindo a necessidade...

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“…Detection of foodborne pathogen bacteria and their toxins can be performed by isotope dilution MALDI-TOF MS, but other, more target-ed investigations and detections of food pathogens such as E. coli, Salmonella sp., Clostridium sp., Mycobacteria sp. and their toxins, as well as possible antibiotic resistance, were performed by high-performance LC (less frequently CE)-MS/MS and by using diff erent instruments (138,141,207,208). At the same time, hybrid type mass spectrometers were used for investigation of proteins in milk as putative biomarkers of milk contamination (208) and antibiotic resistance of potential foodborne pathogens (209,210).…”

Section: Hybrid Mass Spectrometers -Orbitrap and Other Instrumentsmentioning

confidence: 99%

“…and their toxins, as well as possible antibiotic resistance, were performed by high-performance LC (less frequently CE)-MS/MS and by using diff erent instruments (138,141,207,208). At the same time, hybrid type mass spectrometers were used for investigation of proteins in milk as putative biomarkers of milk contamination (208) and antibiotic resistance of potential foodborne pathogens (209,210). Picariello et al (211) and Andjelkovic et al (19) reviewed the use of mass spectrometry-based techniques for the determination of allergens in food.…”

Section: Hybrid Mass Spectrometers -Orbitrap and Other Instrumentsmentioning

confidence: 99%

Foodomics and Food Safety: Where We Are

Andjelković

Gajdošik

Gašo-Sokač

et al. 2017

Food Technol. Biotechnol.

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SummaryThe power of foodomics as a discipline that is now broadly used for quality assurance of food products and adulteration identifi cation, as well as for determining the safety of food, is presented. Concerning sample preparation and application, maintenance of highly sophisticated instruments for both high-performance and high-throughput techniques, and analysis and data interpretation, special att ention has to be paid to the development of skilled analysts. The obtained data shall be integrated under a strong bioinformatics environment. Modern mass spectrometry is an extremely powerful analytical tool since it can provide direct qualitative and quantitative information about a molecule of interest from only a minute amount of sample. Quality of this information is infl uenced by the sample preparation procedure, the type of mass spectrometer used and the analyst's skills. Technical advances are bringing new instruments of increased sensitivity, resolution and speed to the market. Other methods presented here give additional information and can be used as complementary tools to mass spectrometry or for validation of obtained results. Genomics and transcriptomics, as well as affi nity-based methods, still have a broad use in food analysis. Serious drawbacks of some of them, especially the affi nity-based methods, are the cross-reactivity between similar molecules and the infl uence of complex food matrices. However, these techniques can be used for pre-screening in order to reduce the large number of samples. Great progress has been made in the application of bioinformatics in foodomics. These developments enabled processing of large amounts of generated data for both identifi cation and quantifi cation, and for corresponding modeling.

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Ovine subclinical mastitis: Proteomic analysis of whey and milk fat globules unveils putative diagnostic biomarkers in milk

Cited by 34 publications

References 70 publications

Mastitis in sheep – The last 10 years and the future of research

Mastitis in sheep – The last 10 years and the future of research

Somatic cell count in the diagnosis of subclinical mastitis in sheep of different breeds

Foodomics and Food Safety: Where We Are

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