Ovine pulmonary adenocarcinoma as an animal model of progressive lung cancer and the impact of nutritional selenium supply

Humann-Ziehank, Esther; Wolf, Petra; Renko, Kostja; Schomburg, Lutz; Bruegmann, Michael Ludwig; Andreae, Arnim; Brauer, Carsten; Ganter, Martin

doi:10.1016/j.jtemb.2010.10.008

Cited by 12 publications

(3 citation statements)

References 20 publications

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“…Immunohistochemical methods may also be useful in the diagnosis of the disease (Toma et al 2020;De Las Heras et al 2021). The characteristic histopathological (Humann-Ziehank et al 2011;Youssef et al 2015;Mansour et al 2019) and immunohistochemical findings (Hudachek et al 2010;Sun et al 2017;Belalmi et al 2020) of OPA were observed in sheep in this study, which were consistent as previously reported.…”

Section: Discussionsupporting

confidence: 91%

“…Progressive emaciation, weight loss, dyspnoea, polypnoea, coughing, profuse fluid accumulation in the lungs that drains from the nostrils when the sheep's head is lowered are the most important clinical features of OPA (Sonawane et al 2016;Karagianni et al 2019;Mansour et al 2019). In terms of pathological and epidemiological features, OPA is similar to various pulmonary adenocarcinomas in humans, such as bronchoalveolar carcinoma (BAC), therefore, it is a very useful animal tumour model for pulmonary carcinogenesis (Hudachek et al 2010;Humann-Ziehank et al 2011;Sozmen and Beytut 2012). Postmortem findings, histopathological and immunohistochemical examinations are highly reliable in the diagnosis of OPA (Liu et al 2016;De Las Heras et al 2021).…”

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confidence: 99%

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Assessment of MDA and 8-OHdG expressions in ovine pulmonary adenocarcinomas by immunohistochemical and immunofluorescence methods

et al. 2022

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This study aimed to reveal the presence of reactive oxygen species (ROS)-induced lipid peroxidation and DNA damage in ovine pulmonary adenocarcinomas (OPA) by evaluating malondialdehyde (MDA) and 8-hydroxy-2-deoxyguanosine (8-OHdG) expressions by immunohistochemical and immunofluorescence methods. Lung tissue samples were collected from 26 sheep brought to the Pathology Department for routine diagnosis. Lung tissues were fixed in 10% neutral buffered formalin, following routine procedures tissues were stained with hematoxylin and eosin. Avidin-Biotin Peroxidase method was used as immunohistochemical staining. Indirect immunofluorescence method was applied to the sections. Tumoral cells showed acinar, papillary or mixed type patterns. Only 2 of 20 cases metastasized to regional lymph nodes. All OPAs were immune positive for Jaagsiekte Sheep Retrovirus Capsid Protein (JSRV CA), MDA and 8-OHdG. The control group was negative for JSRV CA, MDA and 8-OHdG expressions. Malondialdehyde and 8-OHdG immune positive cells were statistically increased in the OPA group compared to the control group. In conclusion, our results demonstrate that higher MDA and 8-OHdG expressions in sheep with OPA suggest that OPA may be closely related to lipid peroxidation and oxidative DNA damage.

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Section: Discussionsupporting

confidence: 91%

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confidence: 99%

Assessment of MDA and 8-OHdG expressions in ovine pulmonary adenocarcinomas by immunohistochemical and immunofluorescence methods

et al. 2022

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“…Numerous reports in the literature describe the analogous anti-tumor and immunomodulatory effects of some selenium compounds [ 9 , 10 ]. Selenium, as an active center of enzymes involved in oxidative transformations, plays a key role in the resistance mechanisms of plant and animal organisms [ 11 ]. A similar pharmacological effect of selenium and fungal polysaccharides, despite the difference in the mechanism, suggests the possibility of hyperadditive synergism (potentiation of action) of both components [ 12 , 13 , 14 , 15 , 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

Selenium-Containing Exopolysaccharides Isolated from the Culture Medium of Lentinula edodes: Structure and Biological Activity

Górska-Jakubowska

Klimaszewska

Podsadni

et al. 2021

IJMS

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In continuation of our research on the influence of selenium incorporation on the biosynthesis, structure, and immunomodulatory and antioxidant activities of polysaccharides of fungal origin, we have isolated from a post-culture medium of Lentinula edodes a selenium (Se)-containing exopolysaccharide fraction composed mainly of a highly branched 1-6-α-mannoprotein of molecular weight 4.5 × 106 Da, with 15% protein component. The structure of this fraction resembled mannoproteins isolated from yeast and other mushroom cultures, but it was characterized by a significantly higher molecular weight. X-ray absorption fine structure spectral analysis in the near edge region (XANES) suggested that selenium in the Se-exopolysaccharide structure was present mainly at the IV oxidation state. The simulation analysis in the EXAFS region suggested the presence of two oxygen atoms in the region surrounding the selenium. On the grounds of our previous studies, we hypothesized that selenium-enriched exopolysaccharides would possess higher biological activity than the non-Se-enriched reference fraction. To perform structure–activity studies, we conducted the same tests of biological activity as for previously obtained mycelial Se-polyglucans. The Se-enriched exopolysaccharide fraction significantly enhanced cell viability when incubated with normal (human umbilical vein endothelial cells (HUVEC)) cells (but this effect was absent for malignant human cervical HeLa cells) and this fraction also protected the cells from oxidative stress conditions. The results of tests on the proliferation of human peripheral blood mononuclear cells suggested a selective immunosuppressive activity, like previously tested Se-polyglucans isolated from L. edodes mycelium. The Se-exopolysaccharide fraction, in concentrations of 10–100 µg/mL, inhibited human T lymphocyte proliferation induced by mitogens, without significant effects on B lymphocytes. As with previously obtained Se-polyglucans, in the currently tested Se-polymannans, the selenium content increased the biological activity. However, the activity of selenium exopolysaccharides in all tests was significantly lower than that of previously tested mycelial isolates, most likely due to a different mode of selenium binding and its higher degree of oxidation.

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Selenium-Functionalized Molecules (SeFMs) as Potential Drugs and Nutritional Supplements

Abdur

Huang

2015

Topics in Medicinal Chemistry

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Ovine pulmonary adenocarcinoma as an animal model of progressive lung cancer and the impact of nutritional selenium supply

Cited by 12 publications

References 20 publications

Assessment of MDA and 8-OHdG expressions in ovine pulmonary adenocarcinomas by immunohistochemical and immunofluorescence methods

Assessment of MDA and 8-OHdG expressions in ovine pulmonary adenocarcinomas by immunohistochemical and immunofluorescence methods

Selenium-Containing Exopolysaccharides Isolated from the Culture Medium of Lentinula edodes: Structure and Biological Activity

Selenium-Functionalized Molecules (SeFMs) as Potential Drugs and Nutritional Supplements

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