Ovine microsatellites at the OarVH98, OarVHllO, OarVH116, OarVH117 and OarVH130 loci

Hanrahan, V.; Ede, A J; Pierson, C. A.; Hill, Daniel H.

doi:10.1111/j.1365-2052.1993.tb00301.x

Animal Genetics

1993

DOI: 10.1111/j.1365-2052.1993.tb00301.x

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Ovine microsatellites at the OarVH98, OarVHllO, OarVH116, OarVH117 and OarVH130 loci

V. Hanrahan

A J Ede

C. A. Pierson

et al.

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Cited by 8 publications

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“…This opportunity is particularly valuable because reliable estimates of population size for Sitka black-tailed deer have never been available. In addition, DNA-based identification from fecal pellets Levine et al (2000) b Wilson et al (1997) c Bonnet et al (2002) d Bishop et al (1994) e Arevalo et al (1994) f Holder et al (1994) g Mezzelani et al (1995) h Vaiman et al (1994) i Hanrahan et al (1993) j This study potentially will allow researchers to advance understanding of social structure, paternity, kinship, sex ratios, gene flow and phylogeography, all of which are poorly understood for Sitka black-tailed deer. PID is the probability of identity assuming random individuals, and PID SIB is the probability of identity assuming siblings.…”

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confidence: 97%

Individual identification of Sitka black-tailed deer (Odocoileus hemionus sitkensis) using DNA from fecal pellets

Brinkman

Person

Schwartz

et al. 2010

Conservation Genet Resour

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We tested a protocol for extracting DNA from fecal pellets from Sitka black-tailed deer (Odocoileus hemionus sitkensis) and evaluated genotyping performance of previously developed microsatellite markers as well as a suite of new markers designed specifically for this study. We screened 30 microsatellites, and identified 7 (23%) loci including 4 new markers, that fit well into a single multiplex and consistently genotyped deer with low error rates. DNA was extracted from 2,408 fecal-pellet samples. Of those, 1,240 (52%) were genotyped successfully at all 7 markers allowing identification of 634 genetically unique deer. Using DNA from fecal pellets collected in the field was an effective technique for identifying and distinguishing among deer.Keywords Alaska Á DNA Á Feces Á Microsatellites Á Odocoileus hemionus sitkensis Á Sitka black-tailed deer Densely vegetated environments within Southeast Alaska have hindered the collection of basic population parameters on Sitka black-tailed deer (Odocoileus hemionus sitkensis), the most important terrestrial game species in this region.Because of challenges in sampling deer via direct observation (e.g., aerial surveys), we sought non-invasive methods using genetics (Waits and Paetkau 2005) to answer key population questions. We tested a protocol for extracting DNA from fecal pellets and evaluated genotyping performance of previously developed microsatellite markers as well as a new suite of markers designed specifically for Sitka black-tailed deer. Whereas several studies have been conducted on wild carnivores using noninvasive genetic approaches to identify individuals (Waits and Paetkau 2005; Kendall et al. 2008; Schwartz and Monfort 2008), field research identifying individuals using DNA from the feces of wild ungulates has been rare (Flagstad et al. 2000;Gebremedhin et al. 2009 demonstrated that deer feces collected from the rectum are a viable source of DNA. We now seek to assess the utility of fecal DNA from naturally deposited pellets to genotype deer (Odocoileus spp.).During 2006-2008, we collected 4-6 fecal pellets from pellet groups deposited by deer in three watersheds on Prince of Wales Island in southeast Alaska. To minimize DNA degradation from ambient environmental conditions and to maximize DNA recovery, we collected pellets from transects that were cleared of old pellets 10 days earlier. We preserved pellets in 95% ethanol, and stored pellets at room temperature until DNA was extracted. During collection, pellet samples were classified based on appearance as: good (freshly deposited in a clumped distribution with pellets intact, surface with a glossy sheen, and/or a detectable coating of mucus), average (slightly older or more weathered pellet group that still had intact pellets with smooth surfaces, but that lacked a tightly clumped distribution, glossy sheen, and mucus), or poor (spread-out groups with rough-surfaced pellets which were often showing signs of decomposition). Because early experimentation revealed

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confidence: 97%

Individual identification of Sitka black-tailed deer (Odocoileus hemionus sitkensis) using DNA from fecal pellets

Brinkman

Person

Schwartz

et al. 2010

Conservation Genet Resour

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“…Hcterozygosity is calculated as l-(Xp,') where pi = allele frequency. References: 'Bishop et al (1994); 'Steffen et al (1993); "Buchanan & Crawford (1993); "Buchanan & Crawford (1992); 'Hanrahan et al (1993); 'Jamieson (1994 'These multiplex sets are suitable for analysis on a Perkin Elmer ABI automated DNA sequencer model 377 using fluorescently labelled primers; 'Reaction five shows disparity in annealing temperature with other primer sets and is used only when the other four reactions fail to sufficiently clarify possible parentage relationships.…”

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confidence: 99%

A parentage evaluation test in North American Elk (Wapiti) using microsatellites of ovine and bovine origin

1996

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Efficiency of semi‐automated fluorescent multiplex PCRs with 11 microsatellite markers for genetic studies of deer populations

et al. 2002

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Thirty bovine and eight ovine microsatellite primer pairs were tested on four tropical deer species: Eld's and Swamp deer (highly threatened) and Rusa and Vietnamese Sika deer (economically important). Thirty markers gave an amplified product in all four species (78.9%). The number of polymorphic microsatellite markers varied among the species from 14 in Eld's deer (47%) to 20 in Swamp deer (67%). Among them, 11 microsatellite loci were multiplexed in three polymerase chain reactions (PCRs) and labelled with three different fluorochromes that can be loaded in one gel-lane. To test the efficiency of the multiplex, primary genetic studies (mean number of alleles, expected heterozygosities and Fis values) were carried out on four deer populations. Parentage exclusion probability and probability of identity were computed and discussed on a Swamp deer population. These multiplexes PCRs were also tested on several other deer species and subspecies. The aim of this study is to establish a tool useful for genetic studies of population structure and diversity in four tropical deer species which with few modifications can be applied to other species of the genus Cervus.

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Ovine microsatellites at the OarVH98, OarVHllO, OarVH116, OarVH117 and OarVH130 loci

Cited by 8 publications

References 0 publications

Individual identification of Sitka black-tailed deer (Odocoileus hemionus sitkensis) using DNA from fecal pellets

Individual identification of Sitka black-tailed deer (Odocoileus hemionus sitkensis) using DNA from fecal pellets

A parentage evaluation test in North American Elk (Wapiti) using microsatellites of ovine and bovine origin

Efficiency of semi‐automated fluorescent multiplex PCRs with 11 microsatellite markers for genetic studies of deer populations

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