Ovine immune parameters following immunisation against Mycobacterium avium ssp. paratuberculosis using a lipid-based live-cell vaccine

Gillan, Sonia; Hughes, A.D.; O’Brien, Rory; Griffin, J.F.T.

doi:10.1016/j.vetimm.2010.04.020

Cited by 2 publications

(6 citation statements)

References 20 publications

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“…paratuberculosis and other environmental mycobacteria. In the present study, vaccinated calves demonstrated biased reactivities to AvPPD (18.9 Ϯ 5.9) com- (3,9,17,19,20). In the present study, more extensive evaluation of immunologic parameters was performed to further characterize host immune responses to vaccination but, also, to identify potential markers that might be useful in differentiating vaccinated and infected animals.…”

Section: Paratuberculosismentioning

confidence: 75%

“…The rising incidence of Johne's disease in U.S. dairy herds in recent years is forcing producers to consider vaccination more carefully as an additional management tool to prevent the spread of infec- paratuberculosis is generally thought to be the result of Th1-mediated immunity in the host (24), yet vaccination invokes both cell-mediated and humoral immune responses (3,9). With this in mind, we must consider the utility of current immune-based diagnostic tools for the detection of Johne's disease in vaccinated herds.…”

Section: Discussionmentioning

confidence: 99%

“…CD4 CD25 ϩ T cells are highly induced in both experimentally and naturally infected animals, as well as animals vaccinated for paratuberculosis, and represent a highly activated T cell subpopulation (9,22,25,27,33). Coexpression of CD25 and the transcription factor FoxP3 on CD4 ϩ cells defines T regulatory cells, a subpopulation that is critical in controlling inflammatory responses in the host (5).…”

Section: Paratuberculosismentioning

confidence: 99%

“…paratuberculosisspecific serum antibody, thereby confounding their use as diagnostic tools for the detection of M. avium subsp. paratuberculosis infection (9,17,19). However, it is possible that other immunologic parameters may be useful as screening tools that can differentiate M. avium subsp.…”

mentioning

confidence: 99%

“…and ␥␦ T cells and CD4 CD25 T cell populations in recall to antigen stimulation (9,22). A more thorough assessment of host immune responses to vaccination will provide us with information about protective correlates associated with reduced clinical disease.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Mediation of Host Immune Responses after Immunization of Neonatal Calves with a Heat-Killed Mycobacterium avium subsp. paratuberculosis Vaccine

Stabel

Waters

Bannantine

et al. 2011

Clin Vaccine Immunol

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A major drawback of current whole-cell vaccines for Mycobacterium avium subsp. paratuberculosis is the interference with diagnostic tests for bovine tuberculosis (TB) and paratuberculosis. The current study was designed to explore the effects of immunization with a heat-killed whole-cell vaccine (Mycopar) on diagnostic test performance and to characterize host immune responses to vaccination over a 12-month period. Neonatal dairy calves were assigned to treatment groups consisting of (i) controls, not vaccinated (n ‫؍‬ 5), and (

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Section: Paratuberculosismentioning

confidence: 75%

Section: Discussionmentioning

confidence: 99%

Section: Paratuberculosismentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Mediation of Host Immune Responses after Immunization of Neonatal Calves with a Heat-Killed Mycobacterium avium subsp. paratuberculosis Vaccine

Stabel

Waters

Bannantine

et al. 2011

Clin Vaccine Immunol

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Evaluation of protection in a mouse model after vaccination with Mycobacterium avium subsp. paratuberculois protein cocktails

Stabel

Barnhill

Bannantine

et al. 2012

Vaccine

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Whole-cell vaccines successfully reduce signs of clinical disease and fecal shedding of Mycobacterium avium subsp. paratuberculosis (MAP), however, these vaccines have some limitations. The present study was conducted to identify MAP proteins that might be candidates for the development of an improved vaccine. MAP proteins were screened for immunogenicity in naturally infected cattle and selected based upon reactivity in the interferon-γ (IFN-γ) and Western blot assays. Proteins (MAP1087, MAP1204, MAP1272c, and MAP2077c) were arrayed into 4 overlapping cocktails containing 3 proteins each. The efficacy of the proteins within these cocktails as vaccine candidates was evaluated by subcutaneous immunization of mice, followed by challenge with live, virulent MAP. All MAP protein cocktails significantly reduced the recovery of live MAP from the ileum, while cocktails 1 and 3 reduced colonization in the liver. No significant differences were seen in the mesenteric lymph node or spleen, however, cocktail 1 reduced viable MAP in the mesenteric lymph node compared to other treatments. Stimulation of splenocytes upregulated antigen-specific IFN-γ and IL-23 secretion in all treatment groups, regardless of vaccination. Interestingly, IL-4 was moderately downregulated for vaccinates compared to control infected mice. An increase in total CD25 expression was noted for 3 of the 4 vaccinate groups upon stimulation of splenocytes with a whole cell sonicate of MAP, with this effect becoming more significant within CD4CD25+ and CD8CD25+ subpopulations. The present study demonstrated that MAP proteins are useful as vaccine candidates to reduce MAP tissue burden.

show abstract

Ovine immune parameters following immunisation against Mycobacterium avium ssp. paratuberculosis using a lipid-based live-cell vaccine

Cited by 2 publications

References 20 publications

Mediation of Host Immune Responses after Immunization of Neonatal Calves with a Heat-Killed Mycobacterium avium subsp. paratuberculosis Vaccine

Mediation of Host Immune Responses after Immunization of Neonatal Calves with a Heat-Killed Mycobacterium avium subsp. paratuberculosis Vaccine

Evaluation of protection in a mouse model after vaccination with Mycobacterium avium subsp. paratuberculois protein cocktails

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