Ovine cells: Their long-term cultivation and susceptibility to visna virus

Torchio, C; Trowbridge, R. S.

doi:10.1007/bf02615083

Cited by 16 publications

(4 citation statements)

References 21 publications

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“…This may explain the relatively low activity of the human IFN-ctB/D hybrid on the established cell line of monkey Vero cells as compared to human diploid cells. However, the ovine choroid plexus cell line, an established cell line with a long history of subculture in vitro (Torchio & Trowbridge, 1977), had not lost its sensitivity to human IFNs. The human recombinant IFNs had even greater activity on ovine cells than on homologous cells, in accordance with earlier results (Yilma, 1983).…”

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confidence: 99%

A Recombinant Human Interferon- B/D Hybrid with a Broad Host-range

Horisberger

Staritzky

1987

Journal of General Virology

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SUMMARYA recombinant interferon (IFN) hybrid has been found to have a broad host-range of activity in an antiviral assay (plaque reduction of vesicular stomatitis virus) and also high efficacy as an antiviral agent in at least 12 different animal cell species. The IFN hybrid consists of amino acids 1 to 60 from HulFN-c~B and amino acids 61 to 166 from HuIFN-c~D. The profile of cross-species activity of the IFN-,B/D hybrid has been compared with that of HulFN-c~F, and of the parents HulFN-c~B and -c~D. When both IFN-c~B and -aD were active in a cell species, the hybrid IFN had comparable or better activity than the more active parental IFN. The hybrid shared a broad cross-species activity with IFN-c~D. However, the IFN-c~B/D hybrid was 10-fold more active on human cells, 30-fold more active on rabbit cells, and 50-fold more active on mouse cells than IFN-~D.Numerous studies using crude or partially purified mixtures of interferon (IFN) have shown that these preparations usually exert antiviral activity in a defined variety of cell species (for review, see Stewart, 1981). These studies were refined when recombinant IFN became available, when it was observed that the profile of host-range specificity could vary widely between individual species of IFN (Streuli et al., 1981;Yelverton et al., 1981;. Cross-species efficacy has proved to be a useful biological parameter to discriminate between IFN-c~ hybrids constructed by combining gene fragments of individual species of HulFN-c~. In particular, the spectrum of activity of some IFN-~A/D hybrids is different from that of either parent (Streuli et al., 1981;Rehberg et al., 1982; Weck et al., 1981b). The construction of human hybrid molecules with high specific activity on unrelated animal species might provide a useful tool for studying the biology of the IFN system in various animal species.The purpose of this report is to describe the antiviral properties of a recombinant human IFN hybrid which has not only a very broad host-range of activity but also a high efficacy. The hybrid comprises amino acids 1 to 60 from IFN-c~B and amino acids 61 to 166 from IFN-c~D as previously described (Meister et al., 1986).The types of recombinant IFN (> 90~ pure) used in this study were produced in yeast, and their final purification involved affinity immunochromatography using monoclonal antibodies. IFN titres calibrated against HulFN-c~ (NIH standard G 023-901-527) on human diploid cells were: IFN-c~B/D hybrid (0.5 x 108 IU/mg), IFN-c~B (2.5 x 108 IU/mg), IFN-c~D (4 x 10 ° IU/mg) and IFN-c~F (0.5 x 108 IU/mg). Whenever possible, the target cells were diploid cells with a finite life span in tissue culture. They were obtained by digestion of tissues with a neutral protease (Dispase, Boehringer-Mannheim) at pH 6.7 in phosphate-buffered saline. All cells were propagated in Eagle's MEM supplemented with 10~o foetal bovine serum and antibiotics. Primary cells and established cell lines were subcultured using a solution of trypsin (1:250) in EDTA (Gibco).The cross-species activity of IFN ...

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confidence: 99%

A Recombinant Human Interferon- B/D Hybrid with a Broad Host-range

Horisberger

Staritzky

1987

Journal of General Virology

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“…Samples of synovial membrane and choroid plexus were collected asep-3 68 tically a t post mortem for explant culture using techniques previously described (Crawford et a f 1980;Torchio and Trowbridge 1977). Samples of synovial membrane and choroid plexus were collected asep-3 68 tically a t post mortem for explant culture using techniques previously described (Crawford et a f 1980;Torchio and Trowbridge 1977).…”

Section: Virologymentioning

confidence: 99%

Progressive interstitial pneumonia in goats

Sims¹,

Hale²,

McCormick³

1983

Aust Veterinary J

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Seven Anglo-Nubian goats, 5 months to 3 1/2 years old, developed clinical signs of increased respiratory rate, weight loss and exercise intolerance. Post-mortem examination of the goats revealed extensive consolidation of the lungs involving all lobes. Lesions, consisting of peribronchial and perivascular lymphoid cuffing, accumulations of homogeneous eosinophilic material in alveolar spaces, alveolar epithelialisation and thickening of alveolar septa as a result of lymphocytic infiltration, were detected histologically. A syncytial forming virus was consistently isolated from affected animals in explant cultures of lung, synovial membrane and choroid plexus. This agent had similar growth characteristics to the caprine arthritis-encephalitis (CAE) virus and the possible relationship between the respiratory disease and the diseases caused by the CAE virus is discussed.

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“…Interferon titrations were performed in microplates essentially as described by I-IAVEL5 and VILSEK (7), using human FS-4 ceils and encephalomyoearditis (EMC) virus for ChMlenge, or heterologous sheep ehoroid plexus (SCP tI-B) cells (22) and vesicular stomatitis virus for challenge. To identify the antigenic composition of a given interferon fraction, antiviral activity was measured in the presence of excess antibodies directed against either one or both antigenic species of leukocyte interferon (Le and F).…”

Section: Assaysmentioning

confidence: 99%

Biological properties of human interferon fractions obtained by Blue Sepharose chromatography

Erickson

Dalton

Paucker

1980

Archives of Virology

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Human interferons of lymphoid and nonlymphoid origins were segregated into several fractions by virtue of their elution properties from Blue Sepharose. According to desorption of the main portion of antiviral activity, three major classes could be discerned: 1. Leukocyte interferons eluting predominantly in 0.5 M NaCl buffer, 2. Lymphoblastoid interferons eluting mostly in 1 M NaCl buffer, and 3. Cell culture-derived fibroblast and bladder carcinoma interferons requiring ethylene glycol in addition to 1 M NaCl for elution. Desorption behavior from Blue Sepharose did not necessarily correlate with the presence of specific antigenic markers and no consistent segregation of Le and F antigens in individual fractions was observed. All fractions exhibited comparable activity in heterologous sheep and homologous human cells. Therefore, no distinctive biological features could be associated with multiple interferon species isolated by Blue Sepharose chromatography.

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Ovine cells: Their long-term cultivation and susceptibility to visna virus

Cited by 16 publications

References 21 publications

A Recombinant Human Interferon- B/D Hybrid with a Broad Host-range

A Recombinant Human Interferon- B/D Hybrid with a Broad Host-range

Progressive interstitial pneumonia in goats

Biological properties of human interferon fractions obtained by Blue Sepharose chromatography

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