Oviductal epithelial cells transcriptome and extracellular vesicles characterization during thermoneutral and heat stress conditions in dairy cows

Stamperna, Konstantina; Giannoulis, Themistoklis; Cañón-Beltrán, K.; Dovolou, Eleni; Kalemkeridou, Maria; Nanas, Ioannis; Rizos, Dimitrios; Moutou, Katerina A.; Mamuris, Zissis; Amiridis, G.S.

doi:10.1016/j.theriogenology.2022.04.011

Cited by 7 publications

(6 citation statements)

References 91 publications

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“…In the same study [19], several immune-related genes were found to exhibit differential expression. This result, along with the significant changes observed in progesterone levels between periods, is indicative of the detrimental effects of over-activation of the immune system during HS on fertility potential.…”

Section: Oviduct-gravid Uterusmentioning

confidence: 80%

“…In a recent study [19], a comparative transcriptomic analysis was conducted using RNASeq of BOECs during a thermoneutral period (spring) and a HS period (summer). The results revealed that the main pathways of the expressed genes were associated with protein synthesis, mitochondrial biogenesis, and the adaptive immune system, which aligns with the functions of BOECs.…”

Section: Oviduct-gravid Uterusmentioning

confidence: 99%

“…We have recently shown that the concentration of EVs in the ipsilateral oviduct to the CL at the early stages of the luteal phase (days 3 to 4) is reduced during the hot summer months compared to thermoneutral periods. This impaired embryotrophic condition can be considered as an additional causative factor for summer subfertility in dairy cows [19].…”

Section: Oviduct-gravid Uterusmentioning

confidence: 99%

“…These effects disrupt the balance of reproductive hormones, impair oocyte quality, and hinder proper embryo development. As a result, weak estrus expression, low conception rates, and increased embryo mortality are synergistically observed [16][17][18][19][20]. The aim of this review is to discuss the reproductive endocrinologic implications of heatstressed dairy cows and to focus on the effects of high temperatures on oocyte fertilizing capacity and early embryo development.…”

Section: Introductionmentioning

confidence: 99%

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Heat Stress: A Serious Disruptor of the Reproductive Physiology of Dairy Cows

Dovolou

Giannoulis

Nanas

et al. 2023

Animals

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Global warming is a significant threat to the sustainability and profitability of the dairy sector, not only in tropical or subtropical regions but also in temperate zones where extreme summer temperatures have become a new and challenging reality. Prolonged exposure of dairy cows to high temperatures compromises animal welfare, increases morbidity, and suppresses fertility, resulting in devastating economic losses for farmers. To counteract the deleterious effects of heat stress, cattl e employ various adaptive thermoregulatory mechanisms including molecular, endocrine, physiological, and behavioral responses. These adaptations involve the immediate secretion of heat shock proteins and cortisol, followed by a complex network of disrupted secretion of metabolic and reproductive hormones such as prolactin, ghrelin, ovarian steroid, and pituitary gonadotrophins. While the strategic heat stress mitigation measures can restore milk production through modifications of the microclimate and nutritional interventions, the summer fertility records remain at low levels compared to those of the thermoneutral periods of the year. This is because sustainment of high fertility is a multifaceted process that requires appropriate energy balance, undisrupted mode of various hormones secretion to sustain the maturation and fertilizing competence of the oocyte, the normal development of the early embryo and unhampered maternal—embryo crosstalk. In this review, we summarize the major molecular and endocrine responses to elevated temperatures in dairy cows, as well as the impacts on maturing oocytes and early embryos, and discuss the consequences that heat stress brings about in dairy cattle fertility.

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Section: Oviduct-gravid Uterusmentioning

confidence: 80%

Section: Oviduct-gravid Uterusmentioning

confidence: 99%

Section: Oviduct-gravid Uterusmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Heat Stress: A Serious Disruptor of the Reproductive Physiology of Dairy Cows

Dovolou

Giannoulis

Nanas

et al. 2023

Animals

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“…In addition, the proteomic composition of the oviduct uid greatly varies according to the systemic and topical concentrations of estradiol and progesterone across the cycle in mammalian females [9][10][11]. Although poorly explored, there is growing evidence that the mammalian oviduct is also highly sensitive to environmental factors such as diet habits [12][13][14], heat stress [3,15], or chemical compounds commonly found in our daily life [16,17].…”

Section: Introductionmentioning

confidence: 99%

Oral exposure to bisphenol S is associated with alterations in the oviduct proteome of an ovine model, with aggravated effects in overfed females

Mahé,

Riviere,

Lasserre

et al. 2024

Preprint

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Background Bisphenol S (BPS) is a substitute for bisphenol A in plastic manufacturing and, as a potential endocrine disruptor, may alter the physiology of the oviduct, in which fertilization and early embryo development take place in mammals. The objective of this study was to assess the effect of a daily dietary exposure to BPS combined with a contrasted diet on the oviduct fluid proteome using an ovine model. Results Eighty adult cyclic ewes were allotted to four groups (20/group): overfed (OF) consuming 50 µg/kg/day of BPS in their diet, underfed (UF) consuming 50 µg/kg/day of BPS, and non-exposed controls in each diet group. After three months, the mean body condition score and plasma levels of glucose and non-esterified fatty acids were significantly higher in over- than in underfed females. The proteins in collected OF samples (50 µg) were analyzed by nanoliquid chromatography coupled with tandem mass spectrometry (nanoLC-MS/MS). Overall, 1563 proteins were identified, among which 848 were quantified. Principal component analysis of the data revealed a clear discrimination of samples according to the diet and a segregation between BPS-exposed and non-exposed females in overfed ewes. Hierarchical clustering of differentially abundant proteins (DAPs) identified two clusters of 101 and 78 DAPs according to the diet. Pairwise comparisons between groups revealed a stronger effect of BPS in OF than in UF females (70 vs. 24 DAPs) and a stronger effect of the diet in BPS-exposed than non-exposed females (56 vs. 36 DAPs). Functional analysis of DAPs showed an enrichment in metabolic processes, immune system, cell response to stress, and reproductive processes. Conclusions This work highlights for the first time the important impact of BPS on the oviduct proteome, with larger effects seen in OF than UF females. These results, together with previous ones, raise health concerns for everyone and call for a greater regulation of BPS in the food industry.

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