Ovicidal effect of essential oils of Lippia alba, Lippia sidoides and Lippia gracilis on the acanthocephalan Neoechinorhynchus buttnerae (Eoacanthocephala: Neoechinorhynchidae)

Abstract

Acanthocephalosis in tambaqui Colossoma macropomum culture is a limiting factor for the production of the species, being the most recurrent, which makes it necessary to develop strategies to control parasitosis even in the early stages of parasite development. This study aimed to elucidate the morphological characteristics of eggs of the acanthocephalan Neoechinorhynchus buttnerae and the ovicidal effect of the essential oils of Lippia alba, Lippia sidoides, and Lippia gracilis. The parasites were collected from hosts naturally infected with N. buttnerae, and the eggs obtained from the female 'parasites' abdominal cavity were added to 12‐well plates containing the essential oils at different dilutions. After the eggs (N = 300) were separated and measured (length and width) with the aid of an optical microscope coupled to a digital program, the viability of the treated eggs was monitored 24 h after treatments, L. gracilis essential oil was 100% effective at the highest concentration, whereas L. sidoides essential oil showed over 50% efficacy at 2 mg/ml, reaching 100% efficacy at all higher concentrations. Lippia. alba essential oil did not show satisfactory efficacy in preventing N. buttnerae egg hatching.

Anthelmintic Efficacy of Palmarosa Oil and Curcuma Oil against the Fish Ectoparasite Gyrodactylus kobayashii (monogenean)

“…Natural or plant-derived herbal products and their derivatives have recently gained more attention as an alternative method for monogenean management [13,14]. Essential oils (EOs) are the secondary metabolites of herbal medicines, and some EOs have been reported to be effective in eliminating monogenean infections [15][16][17][18]. For example, the EO of lemongrass (Cymbopogon citratus) showed 100% anthelmintic efficacy against monogenean parasites of the tambaqui (Colossoma macropomum) in the in vitro assays [19].…”