Overview of typing techniques as molecular epidemiology tools for bacterial characterization

Almasian-Tehrani, Nasim; Alebouyeh, Masoud; Armin, Shahnaz; Soleimani, Neda; Azimi, Leila; Shaker-Darabad, Roozbeh

doi:10.55705/cmbr.2021.143413.1016

Cited by 10 publications

(10 citation statements)

References 28 publications

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“…Blood banks have invested considerable time and effort in the adoption as well as the optimization of the efficiency of various contaminant detection methods. An analytical test for the detection of bacteria must not only be generic, in order to detect all possible strains but also highly sensitive to detect the presence of bacteria at very low numbers [15][16][17][18]. Recently, the standard techniques for automated bacterial detection are based on the growth of bacteria such as Staphylococcus epidermis and Staphylococcus aureus in a nonspecific medium both in aerobic and anaerobic conditions.…”

Section: Hospitalmentioning

confidence: 99%

Surface modification of SiO2 nanoparticles for bacterial decontaminations of blood products

Alavi¹,

Hamblin²,

Mozafari³

et al. 2022

Cell. Mol. Biomed. Rep.

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Section: Hospitalmentioning

confidence: 99%

Surface modification of SiO2 nanoparticles for bacterial decontaminations of blood products

Alavi¹,

Hamblin²,

Mozafari³

et al. 2022

Cell. Mol. Biomed. Rep.

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“…Recently, finding an effective therapy for hindering bacterial, fungal, and viral infections have obtained high attention [1][2][3][4][5]. Tuberculosis is a kind of bacterial infection that is caused by Mycrobacterium tuberculosis [6].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Active Pulmonary Tuberculosis Among Women With Diabetes

Behzadmehr

Keikhaie

2022

Cell. Mol. Biomed. Rep.

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Diabetes is one of the most common non-contagious diseases in the world. This disease is the fourth or fifth cause of death in most developed countries. The relationship between tuberculosis and diabetes had been introduced years ago and diabetes is considered a threatening factor in tuberculosis in the research history. Not only tuberculosis is prevalent among diabetic persons, but also diabetes can affect the appearance of imaging of tuberculosis. This is a kind of analytic study, a case-control study, which was carried out between the years 2014 and 2015 in Zabol City. In this study, the radiographic findings from the patients suffering from pulmonary tuberculosis in diabetic patients and not diabetic patients are compared. These radiographic were handed to a radiologist and the radiologic findings of these graphs from the anatomic location (upper and lower half of the right and left bellows), Presence or absence of cavities, nodules, density and pleural involvement were recorded and compared with the diabetic and non-diabetic group. After gathering of the data using SPSS software, descriptive statistics were presented in the form of (frequency, percent) graphs and a chisquare test (p<0.05) was used to analyze and comparing of the results of diabetic and un-diabetic patients. The population of the study consisted of Chest radiographs for 124 TB patients which 61 (49.19 %) were suffering from diabetics. In this study, 45 (71.43%) non-diabetic and 42 (65.85%) diabetic patients were women (p=0.7). There were 12 diabetic TB patients (19.67%) and 3 nondiabetic-TB patients (4.76%) with the consolidation of middle part of left lung (p=0.01) and 8 (13.11%) diabetic TB and 1 (1.59%) nondiabetic TB with reticulonodular infiltration of lower part of left lung (p=0.02). There was no significant difference in the rest of the radiographic results. The findings of this study indicate that reticulonodular infiltration and consolidation of lower &middle parts of the lung in TB diabetic patients is more than in TB nondiabetic patients and diabetes can affect the findings of pulmonary tuberculosis radiography.

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“…The traditional cultivation method has always been microbial as the excellent and technique for biological detection (14); so far, it is still used as mycoplasma isolated in the world. The culture method is based on the directed culture of mycoplasma cells, and the user can promote some supplements into the medium for mycoplasma growth, On the 1-2 week after the initial inoculation, the subculture of the liquid medium was inoculated on the agar plate (15).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of culturing and molecular assay for detection of Mycoplasma gallisepticum in chicken suffering from chronic respiratory disease

Hamzah¹,

Ayed²,

Muhammed³

2022

Cell Mol Biol (Noisy-le-grand)

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Mycoplasma gallisepticum (M. gallisepticum) is a bacterium that causes chronic respiratory disease (CRD) and infectious sinusitis (IS) in chickens and turkeys. Therefore, rapid and immediate diagnosis or regular detection of Mycoplasma may be of great help to early detection. 120 chicken layers, Within Karbala city, were carried out during their laying period on breeding flocks. The study proposed a promising method for isolation of M. gallisepticum, 120 tracheal swabs and blood samples from chickens in different dairy farms were used to analyze M. gallisepticum utility of PCR and culture. Compared with ELISA anti-IgG M. gallisepticum, the clinical specificity of PCR detection is 89.66%, the sensitivity is 86.36%, and the kappa coefficient is 0.817. Compared with the culture method, the specificity is 100%, the specificity is 45%, and the kappa coefficient is 0.543. Demonstrate the method's effectiveness and applicability as a standard method for mycoplasmas field diagnosis.

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Overview of typing techniques as molecular epidemiology tools for bacterial characterization

Cited by 10 publications

References 28 publications

Surface modification of SiO2 nanoparticles for bacterial decontaminations of blood products

Surface modification of SiO2 nanoparticles for bacterial decontaminations of blood products

Evaluation of Active Pulmonary Tuberculosis Among Women With Diabetes

Evaluation of culturing and molecular assay for detection of Mycoplasma gallisepticum in chicken suffering from chronic respiratory disease

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