Overview of the status and applications of capillary electrophoresis to the analysis of small molecules

Altria, Kevin D.; Elder, David

doi:10.1016/j.chroma.2003.10.054

Cited by 122 publications

(68 citation statements)

References 96 publications

(93 reference statements)

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“…Multicapillary CE instruments have been applied primarily to DNA sequencing, which in general presents no problem with sensitivity issues because of concentration amplification provided by PCR. Some commercial multicapillary CE instruments can be adapted for high-throughput analysis of proteins and peptides, although little information has been reported in this area (40). To compensate for such low throughput, investigators have immobilized multiple capture antibodies in the forepart of the same capillary, enabling selection and capture of several different analytes per single sample introduction (37 ).…”

Section: Applicationsmentioning

confidence: 99%

Immunoaffinity CE for Proteomics Studies.

Guzman

Phillips²

2005

Anal. Chem.

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Section: Applicationsmentioning

confidence: 99%

Immunoaffinity CE for Proteomics Studies.

Guzman

Phillips²

2005

Anal. Chem.

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“…[4][5][6][7][8][9][10][11][12] The mass sensitivity (S m ), defined as the minimum amount of analyte detectable, of NMR can be expressed as (2) where ω 0 is the operating frequency of the NMR system, M is the number of moles of analyte within the observe volume of the NMR detector, t is the total data acquisition time, and d c is the diameter of the NMR radio frequency coil, which is usually either a solenoid or saddle geometry. Equation 2 shows that one ideally performs experiments at the highest magnetic field strength, with the maximum concentration of analyte, and the smallest diameter NMR coil.…”

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confidence: 99%

Magnetic Resonance Microimaging and Numerical Simulations of Velocity Fields Inside Enlarged Flow Cells Used for Coupled NMR Microseparations

Zhang

Webb

2005

Anal. Chem.

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The coupling of various chemical microseparation methods with small-scale NMR detection is a growing area in analytical chemistry. The formation of enlarged flow cells within the active volume of the NMR detector can significantly increase the coil filling factor and hence the signal-to-noise ratio of the NMR spectra. However, flow cells can also lead to deterioration of the separation efficiency due to the development of complex flow patterns, the form of which depend on the particular geometry of the flow cell and the flow rate used. In this study, we investigated the flow characteristics in different flow cell geometries relevant to the coupling of capillary liquid chromatography and NMR. Computational fluid dynamics was used to simulate fluid flow inside flow cells with a volume of ~ 1 µL. Magnetic resonance microimaging was used to measure experimentally the velocity fields inside these flow cells. The results showed good agreement between experiment and simulation and demonstrated that a relatively gradual expansion and contraction is necessary to avoid areas of weak recirculation and strong radial velocities, both of which can potentially compromise separation efficiency.An essential component in the purification and analysis of unknown compounds is efficient separation of individual components from an often complex chemical or biological system. Advances in microseparations, include techniques such as capillary liquid chromatography (cLC), 1 capillary electrophoresis (CE), 2 and capillary electrochromatography 3 have played a large role in improving the separation efficiency. The chromatographic dilution (D) of cLC, for example, is given by (1) where C 0 is the initial concentration of the analyte, C max is the final compound concentration at the peak maximum, r is the column radius, ϵ is the column porosity, k is the retention factor, L is the column length, H is the column plate height, and V inj is the injected sample volume. Equation 1 shows that a smaller column radius results in less chromatographic dilution and higher concentration elution peaks. The smaller scale is also much more amenable to very rapid separations. In addition to these general benefits of microseparations, in electrophoretic separations the high surface area-to-volume ratio of the small capillaries used in CE systems,

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“…Em análises agroquímicas, a aplicação da eletroforese capilar tem mostrado sucesso na determinação de muitos fungicidas, herbicidas, inseticidas, acaricidas em água, solo e gêneros alimentícios. Resíduos de fármacos, tipicamente antibióticos, em alguns gêneros alimentícios de origem animal têm também sido mensurados por eletroforese capilar, como exemplo a oxitetraciclina em tecido de porco e a enrofloxacina em amostras de frango [31] . O primeiro estudo com uso de eletroforese capilar na análise de antibióticos da classe dos aminoglicosídeos utilizou detecção espectrofotométrica indireta, em pH ácido sob condições de polaridade invertida.…”

Section: Técnicas De Separação Eletroforese Capilarunclassified

Antimicrobianos em alimentos de origem vegetal - uma revisão

Maia

Rath

Reyes

2015

Segur. Aliment. Nutr.

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ResumoDoenças de origem bacteriana em frutas e hortaliças ocorrem no campo ou após a colheita, reduzem a produção e afetam a qualidade do produto para a comercialização e, muitas vezes, causam prejuízos econômicos elevados ao agricultor. O alto teor de água de frutas e hortaliças torna esses vegetais sensíveis ao ataque de bactérias. Para prevenir as perdas econômicas decorrentes de doenças bacterianas em alimentos de origem vegetal, o controle químico é feito com o uso de bactericidas agrícolas como a oxitetraciclina e estreptomicina. Esta revisão tem por objetivo abordar os antimicrobianos permitidos pela legislação brasileira para o uso em lavouras, assim como aspectos de legislação e de métodos analíticos utilizados para avaliação da presença de resíduos dos antimicrobianos em vegetais.Palavras-chave: antimicrobianos, vegetais, oxitetraciclina, estreptomicina. AbstractBacterial diseases in fruits and vegetables occur in the field or after the harvest, reduce the production and affect the product quality for the commercialization and, many times, cause high economical losses to the agriculturist. The high water content of fruits and vegetables makes these foods sensible to the bacterial attack. To prevent economic losses from bacterial disease in foods of vegetal origin, the chemical control is made with the use of agricultural bactericidal such as oxytetracycline and streptomycin. This paper presents a review about the antimicrobials permitted by the Brazilian legislation to be used in farming, as well as aspects related to the legislation and analytical methods used for the evaluation of the presence of antimicrobial residues in vegetables.

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Overview of the status and applications of capillary electrophoresis to the analysis of small molecules

Cited by 122 publications

References 96 publications

Immunoaffinity CE for Proteomics Studies.

Immunoaffinity CE for Proteomics Studies.

Magnetic Resonance Microimaging and Numerical Simulations of Velocity Fields Inside Enlarged Flow Cells Used for Coupled NMR Microseparations

Antimicrobianos em alimentos de origem vegetal - uma revisão

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