Overview of the Neurocytology of Ganglia and Identification of Putative Serotonin- and Dopamine-Secreting Neurons in the Bivalve Peppery Furrow Shell (<i>Scrobicularia plana</i>)

Tantiwisawaruji, Sukanlaya; Malhão, Fernanda; Lopes, Célia; Silva, Ana; Kovitvadhi, Uthaiwan; Pardal, Miguel Ângelo; Rocha, Maria João; Rocha, Eduardo

doi:10.2983/035.036.0304

Cited by 3 publications

(4 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The ganglia have the three previously identified and characterized distinct cell types in S. plana [ 19 ]: neurons (larger and smaller somata), neuroglia, and pigmented cells ( Figure 3 ). The neurons are unequivocally recognizable by their bigger size, having a single roundish nucleus, typically with one nucleolus, and abundant and well-stained cytoplasm ( Figure 3 A).…”

Section: Resultsmentioning

confidence: 85%

“…As to cellularity, the data are given in various forms, including by splitting the N in numbers per cellular contingents perfectly defined by morphology, as follows: (a) neurons with larger and smaller cell bodies, (b) fusiform, roundish, and triangularly (cell body) shaped glial cells, and (c) pigmented neural cells. These phenotypes were earlier specified in the species [ 19 ]. The concept and identification of neurons with larger and smaller somata were absolute because each counted cell body with well-stained cytoplasm was tagged as a “large” (oval/round, often with larger cytoplasmic granules, Ø of ≈ 18 × 25 µm) or as a “small” neuron (oval/round, thick heterochromatin rim, Ø ≈ 7 × 15 µm).…”

Section: Methodsmentioning

confidence: 99%

“…Direct and indirect evidence supports the idea for the latter. In the peppery furrow shell, Scrobicularia plana , serotonin immunostaining showed stronger intensity and expression extension in the visceral ganglia of mature animals than in immature ones [ 19 ]. In the pen shell, Atrina pectinate , a neuropepetide detected in visceral ganglia was considered to promote oocyte maturation and sperm motility activation [ 20 ].…”

Section: Introductionmentioning

confidence: 99%

“…Some disrupt the nervous system, and better descriptions of its structure in bivalves will contribute to better diagnosis, appreciation and prediction of the neurotoxic impacts described in these organisms [ 36 , 37 , 38 ]. The species’ central nervous system follows the general organization seen in bivalves [ 19 ]: three pairs of capsulated ganglia—the right and left cerebral (i.e., cerebropleural) ganglia, the pedal (fused in one) ganglia, and the visceral ganglia (fused in one)—connected by nerves (i.e., nerve cords) ( Figure 1 ). However, the exact location, the relative size of ganglia, and the degree of ganglionic fusion may vary with the bivalve species [ 39 , 40 ].…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A Stereological Study of the Three Types of Ganglia of Male, Female, and Undifferentiated Scrobicularia plana (Bivalvia)

Tantiwisawaruji

Rocha

Silva

et al. 2022

Animals

Self Cite

View full text Add to dashboard Cite

Neurotransmitters modulate gonadal maturation in bivalves. However, it remains unclear whether there are differences in the nervous system structure between sexes, maturation, and ganglia. Therefore, a stereological study was conducted on the ganglia of adult peppery furrow shell (Scrobicularia plana). Equal-sized males, females, and undifferentiated (gamete absence) animals were fixed with 10% formalin and processed for light microscopy. They were serially cut into 35 µm paraffin thick sections and stained with hematoxylin-eosin. Sections with cerebral (cerebropleural), pedal, and visceral ganglia were studied. The parameters estimated were the volumes of the ganglia, the total and relative volumes of their cortex (outer layer) and medulla (neuropil), and the total number of cells (neurons, glia, and pigmented) per ganglia and compartment. The volumes and numbers were estimated, respectively, by the Cavalieri principle and by the optical fractionator. Females show a larger glia to neuron numerical ratio. Further, females have a greater ganglionic volume than undifferentiated adults, with males showing intermediate values. These facts indicate that the ganglia size is related somehow to maturation. The cell size forms the basis of the differences because total cellularity is equal among the groups. The three ganglion types differ in total volumes and the volume ratio of the cortex versus the medulla. The greater volumes of the pedal ganglia (vis-a-vis the cerebral ones) and of the visceral ganglia (in relation to all others) imply more voluminous cortexes and medullae, but more neuronal and non-neuronal cells only in the visceral. The new fundamental data can help interpret bivalve neurophysiology.

show abstract

Section: Resultsmentioning

confidence: 85%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A Stereological Study of the Three Types of Ganglia of Male, Female, and Undifferentiated Scrobicularia plana (Bivalvia)

Tantiwisawaruji

Rocha

Silva

et al. 2022

Animals

Self Cite

View full text Add to dashboard Cite

show abstract

Chronic exposure to bisphenol A induces behavioural, neurochemical, histological, and ultrastructural alterations in the ganglia tissue of the date mussels Lithophaga lithophaga

Abd Elkader,

Al-Shami

2023

Environ Sci Pollut Res

View full text Add to dashboard Cite

Bisphenol A (BPA), a common plastic additive, has been demonstrated mechanistically to be a potential endocrine disruptor and to affect a variety of body functions in organisms. Although previous research has shown that BPA is toxic to aquatic organisms, the mechanism of neurotoxic effects in marine bivalves remains unknown. The current study aimed to elucidate the neurotoxic effects of BPA when administered at different concentrations (0.25, 1, 2, and 5 µg/L) for twenty-eight days in the ganglia of a bivalve model, the Mediterranean mussel (Lithophaga lithophaga), which is an ecologically and economically important human food source of bivalve species in the Mediterranean Sea. Our findings revealed an increase in behavioural disturbances and malondialdehyde levels in treated mussel ganglia compared to the control group. Furthermore, superoxide dismutase activity increased in the ganglia of L. lithophaga treated with 0.25 and 2 µg/L. However, at BPA concentrations of 1 and 5 µg/L, SOD activity was significantly reduced, as was total glutathione concentration. BPA causes neurotoxicity, as evidenced by concentration-dependent inhibition of acetylcholinesterase, dopamine, and serotonin. After chronic exposure to BPA, neurons showed distortion of the neuronal cell body and varying degrees of pyknosis. The ultrastructure changes in BPA-treated groups revealed the lightening of the nucleoplasm and a shrunken nuclear envelope. Overall, our findings suggest that BPA exposure altered antioxidation, neurochemical biomarkers, histopathological, and ultrastructural properties, resulting in behavioural changes. As a result, our findings provide a basis for further study into the toxicity of BPA in marine bivalves.

show abstract

Acetylcholinesterase and dopamine inhibition suppress the filtration rate, burrowing behaviours, and immunological responses induced by bisphenol A in the hemocytes and gills of date mussels, Lithophaga lithophaga

Elkader,

Al-Shami

2024

Aquatic Toxicology

View full text Add to dashboard Cite

Overview of the Neurocytology of Ganglia and Identification of Putative Serotonin- and Dopamine-Secreting Neurons in the Bivalve Peppery Furrow Shell (Scrobicularia plana)

Cited by 3 publications

References 47 publications

A Stereological Study of the Three Types of Ganglia of Male, Female, and Undifferentiated Scrobicularia plana (Bivalvia)

A Stereological Study of the Three Types of Ganglia of Male, Female, and Undifferentiated Scrobicularia plana (Bivalvia)

Chronic exposure to bisphenol A induces behavioural, neurochemical, histological, and ultrastructural alterations in the ganglia tissue of the date mussels Lithophaga lithophaga

Acetylcholinesterase and dopamine inhibition suppress the filtration rate, burrowing behaviours, and immunological responses induced by bisphenol A in the hemocytes and gills of date mussels, Lithophaga lithophaga

Contact Info

Product

Resources

About