Overview of the Cellular Stress Responses Involved in Fatty Acid Overproduction in E. coli

Sawant, Neha; Singh, Harinder; Appukuttan, Deepti

doi:10.1007/s12033-021-00426-4

Cited by 7 publications

(2 citation statements)

References 114 publications

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“…All three TSs reduced luminescence. This effect may result from the dependence of luminescence on decanal, a metabolic derivative that relies on fatty acid synthesis, which is sensitive to cellular stress . Regardless of its mechanistic basis, this sensitivity precludes the facile extension of luminescent B2H systems to screens of terpenoid pathways.…”

Section: Resultsmentioning

confidence: 99%

Genetically Encoded Detection of Biosynthetic Protease Inhibitors

et al. 2022

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Proteases are an important class of drug targets that continue to drive inhibitor discovery. These enzymes are prone to resistance mutations, yet their promise for treating viral diseases and other disorders continues to grow. This study develops a general approach for detecting microbially synthesized protease inhibitors and uses it to screen terpenoid pathways for inhibitory compounds. The detection scheme relies on a bacterial two-hybrid (B2H) system that links protease inactivation to the transcription of a swappable reporter gene. This system, which can accomodate multiple biochemical outputs (i.e., luminescence and antibiotic resistance), permitted the facile incorporation of four disease-relevant proteases. A B2H designed to detect the inactivation of the main protease of severe acute respiratory syndrome coronavirus 2 enabled the identification of a terpenoid inhibitor of modest potency. An analysis of multiple pathways that make this terpenoid, however, suggested that its production was necessary but not sufficient to confer a survival advantage in growth-coupled assays. This finding highlights an important challenge associated with the use of genetic selection to search for inhibitorsnotably, the influence of pathway toxicityand underlines the value of including multiple pathways with overlapping product profiles in pathway screens. This study provides a detailed experimental framework for using microbes to screen libraries of biosynthetic pathways for targeted protease inhibitors.

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Section: Resultsmentioning

confidence: 99%

Genetically Encoded Detection of Biosynthetic Protease Inhibitors

et al. 2022

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“…Thus, poor IAA accumulation is a primary reason for the reduced branching of lateral roots. Sub-optimal conditions give rise to imbalanced osmotic potential, which greatly inhibits active osmoregulation and triggers osmotic and oxidative stress (Sawant et al, 2021). The molecular expression of TaSnRK2.7 is considered a multifunctional regulatory factor involved in carbohydrate metabolism and reducing osmotic potential in wheat plants under sub-optimal temperature conditions (Zhang et al, 2011).…”

Section: Molecular Expressions and Regulationmentioning

confidence: 99%

Effects of Low Temperature Stress on Source–Sink Organs in Wheat and Phosphorus Mitigation Strategies

Hassan

Sun

et al. 2022

Front. Plant Sci.

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The 21st century presents many challenges to mankind, including climate change, fast growing human population, and serious concerns over food security. Wheat is a leading cereal crop that largely fulfills the global food needs. Low temperature stress accompanied by nutrient-starved soils is badly disrupting the source–sink relationship of wheat, thus causing an acute decline in final yield and deteriorating the grain quality. This review paper aimed to understand how low temperature stress affects wheat source–sink organs (i.e., leaves, roots, and spikes) and how phosphorus application reliefs in alleviating its harmful consequences. Also, we discussed mitigation strategies to enhance wheat capacity to adapt to varying temperature extremes and made rational recommendations based on modern agronomic and breeding approaches. Therefore, this study is likely to establish a solid foundation for improving the tolerance to low temperature stress and to improve its phosphorus utilization efficiency in wheat.

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