Overview of spatio-temporal distribution inferred by multi-locus sequence typing of Taylorella equigenitalis isolated worldwide from 1977 to 2018 in equidae

Duquesne, Fabien; Merlin, Aurélie; Pérez-Cobo, Iratxe; Sedlák, Kamil; Melzer, Falk; Overesch, Gudrun; Frétin, David; Iwaniak, Wojciech; Breuil, Marie-France; Wernery, Ulrich; Hicks, Jessica A.; Agüero-García, Montserrat; Frías-Serrano, Nieves; Miguel-Ibáñez, Elena San; Patrasová, Eva; Waldvogel, Andreas; Szulowski, Krzysztof; Joseph, Marina; Jeeba, John; Shanty, Jose; Varghese, Preethamol; Hans, Aymeric; Pétry, Sandrine

doi:10.1016/j.vetmic.2020.108597

Cited by 5 publications

(2 citation statements)

References 29 publications

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“…A visual geoBURST analysis was constructed between the STs of isolates and other strains from diverse sources at the level of TLV. In Figure 3, the distribution of STs in different clone complexes (CCs) reflects the correlation between genetic distance and evolutionary relationship between close relatives of STs [28]. At the threshold of TLV, isolates and standard strain STs were divided into three main CCs, two secondary CCs, and several singletons.…”

Section: Multilocus Sequence Typing (Mlst) Of Isolatesmentioning

confidence: 99%

Identification of Burkholderia gladioli pv. cocovenenans in Black Fungus and Efficient Recognition of Bongkrekic Acid and Toxoflavin Producing Phenotype by Back Propagation Neural Network

Niu,

Song,

Hao

et al. 2024

Foods

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Burkholderia gladioli pv. cocovenenans is a serious safety issue in black fungus due to the deadly toxin, bongkrekic acid. This has triggered the demand for an efficient toxigenic phenotype recognition method. The objective of this study is to develop an efficient method for the recognition of toxin-producing B. gladioli strains. The potential of multilocus sequence typing and a back propagation neural network for the recognition of toxigenic B. cocovenenans was explored for the first time. The virulent strains were isolated from a black fungus cultivation environment in Qinba Mountain area, Shaanxi, China. A comprehensive evaluation of toxigenic capability of 26 isolates were conducted using Ultra Performance Liquid Chromatography for determination of bongkrekic acid and toxoflavin production in different culturing conditions and foods. The isolates produced bongkrekic acid in the range of 0.05–6.24 mg/L in black fungus and a highly toxin-producing strain generated 201.86 mg/L bongkrekic acid and 45.26 mg/L toxoflavin in co-cultivation with Rhizopus oryzae on PDA medium. Multilocus sequence typing phylogeny (MLST) analysis showed that housekeeping gene sequences have a certain relationship with a strain toxigenic phenotype. We developed a well-trained, back-propagation neutral network for prediction of toxigenic phenotype in B. gladioli based on MLST sequences with an accuracy of 100% in the training set and an accuracy of 86.7% in external test set strains. The BP neutral network offers a highly efficient approach to predict toxigenic phenotype of strains and contributes to hazard detection and safety surveillance.

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Section: Multilocus Sequence Typing (Mlst) Of Isolatesmentioning

confidence: 99%

Identification of Burkholderia gladioli pv. cocovenenans in Black Fungus and Efficient Recognition of Bongkrekic Acid and Toxoflavin Producing Phenotype by Back Propagation Neural Network

Niu,

Song,

Hao

et al. 2024

Foods

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“…CEM cases were first confirmed in Japan in 1980 [13] and spread to the Thoroughbred population, but was eradicated from Japanese Thoroughbred horses by 2010 as a result of comprehensive infestation control measures [1]. However, sporadic cases of CEM have been identified around the world in recent years [8,10,15]. The detection of T. equigenitalis through quarantine is most important in CEM-free countries such as Japan.…”

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confidence: 99%

Comparison of seven nucleic acid amplification tests for detection of <i>Taylorella equigenitalis</i>

Kinoshita

Kakoi

Ishige

et al. 2022

The Journal of Veterinary Medical Science

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Taylorella equigenitalis causes contagious equine metritis. Here we compared seven nucleic acid amplification tests for T. equigenitalis to select a rapid and reliable diagnostic method. The 95% detection limits of each assay varied greatly: real-time PCR had the lowest detection limit (0.77 fg/reaction); those of some of the conventional PCRs (cPCRs) were >100 fg/reaction. In experimentally infected samples, real-time PCR and semi-nested PCR showed the highest positive numbers (33 out of 42 samples), but two of the cPCRs detected only 2 and 7 positive results. Our results indicate that the use of sensitive molecular assays is important for the efficient detection of T. equigenitalis in clinical samples.

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Validation of an Easy Handling Sample Preparation and Triplex Real Time PCR for Rapid Detection of T. equigenitalis and Other Organisms Associated with Endometritis in Mares

Léon

Versmisse²,

Despois³

et al. 2020

Journal of Equine Veterinary Science

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Overview of spatio-temporal distribution inferred by multi-locus sequence typing of Taylorella equigenitalis isolated worldwide from 1977 to 2018 in equidae

Cited by 5 publications

References 29 publications

Identification of Burkholderia gladioli pv. cocovenenans in Black Fungus and Efficient Recognition of Bongkrekic Acid and Toxoflavin Producing Phenotype by Back Propagation Neural Network

Identification of Burkholderia gladioli pv. cocovenenans in Black Fungus and Efficient Recognition of Bongkrekic Acid and Toxoflavin Producing Phenotype by Back Propagation Neural Network

Comparison of seven nucleic acid amplification tests for detection of <i>Taylorella equigenitalis</i>

Validation of an Easy Handling Sample Preparation and Triplex Real Time PCR for Rapid Detection of T. equigenitalis and Other Organisms Associated with Endometritis in Mares

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