Overview of methodologies for the culturing, recovery and detection of
            <i>Campylobacter</i>

N, Marcela Soto-Beltrá; Lee, Bertram G.; Amézquita-López, Bianca A.; Quiñones, Beatriz

doi:10.1080/09603123.2022.2029366

Cited by 9 publications

(10 citation statements)

References 87 publications

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“…Our data revealed a higher prevalence of Campylobacter in the adult group between 19 and 65 years old with a median age in cases of 20 years. This age distribution is in accordance with observations from several industrialized countries [ 4 , 11 , 26 ]. Similar trends have been found in urban communities from developing countries [ 11 ].…”

Section: Discussionsupporting

confidence: 92%

“…Campylobacter jejuni is the most prevalent human pathogen. Campylobacteriosis has been the most frequent gastrointestinal infection reported in Europe during the last decade, with an incidence of 59.7 cases per 100,000 persons in 2019 [ 3 , 4 ]. Similar results have been reported from the USA, with an incidence of 19.5/100,000 during the same period [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

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Campylobacter spp. Prevalence in Santiago, Chile: A Study Based on Molecular Detection in Clinical Stool Samples from 2014 to 2019

et al. 2023

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Campylobacter spp. is an emerging cause of infectious diarrhea worldwide. In South American countries such as Chile, its prevalence is underestimated due to inadequate detection methods. Gastrointestinal multiplex PCR panels (GMP) permit rapid and sensitive detection of bacterial pathogens and provide important epidemiological information. This study aimed to analyze Campylobacter epidemiology using the results of molecular methods and to compare molecular detection results to those of culture methods. We performed a retrospective, descriptive analysis of Campylobacter spp. detected in clinical stool samples between 2014–2019 by GMP and culture. Within 16,582 specimens examined by GMP, Campylobacter was the most prevalent enteropathogenic bacteria (8.5%), followed by Salmonella spp. (3.9%), Shigella spp./enteroinvasive Escherichia coli (EIEC) (1.9%), and Yersinia enterocolitica (0.8%). The highest Campylobacter prevalence occurred in 2014/2015. Campylobacteriosis affected more males (57.2%) and adults from 19–65 years (47.9%) and showed a bimodal seasonality with summer and winter peaks. In 11,251 routine stool cultures, Campylobacter spp. was detected in 4.6%, mostly C. jejuni (89.6%). Among 4533 samples tested by GMP and culture in parallel, GMP showed a superior sensitivity (99.1% versus 50%, respectively). The study suggests that Campylobacter spp. is the most frequent bacterial enteropathogen in Chile.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Campylobacter spp. Prevalence in Santiago, Chile: A Study Based on Molecular Detection in Clinical Stool Samples from 2014 to 2019

et al. 2023

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“…Briefly, previously preserved putative Campylobacter isolates were revived by subculturing on blood agar plates with selective supplement (BASs) at 42 °C for 24 hours. e colonies (3)(4)(5) were then suspended in sterile distilled water in an Eppendorf tube.…”

Section: Extraction Of Bacterial Dna and Pcr Identification Of Thementioning

confidence: 99%

“…ermophilic campylobacters, particularly C. jejuni and C. coli, are of most clinical relevance and are also most frequently encountered. Other species C. concisus, C. lari, C. upsaliensis, and C. ureolyticus are described as "emerging Campylobacter species" and have been underrated as causal agents of human gastroenteritis because of biases in the existing identification methods [4,5]. ermotolerant campylobacters are presumed to be commensals in birds and insignificant for avian health, with reports indicating that the bacterium may colonise the palatine lymphoid tissues and the crops in broilers, with higher colonisation in intensive systems [1].…”

Section: Introductionmentioning

confidence: 99%

Seasonal Prevalence and Molecular Identification of Thermophilic Campylobacter from Chicken, Cattle, and Respective Drinking Water in Kajiado County, Kenya

Wanja

Mbuthia

Aboge

et al. 2022

International Journal of Microbiology

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Thermophilic Campylobacter species are a leading cause of human gastroenteritis throughout the world and have been implicated in reproductive disorders (abortion), mastitis, enteritis, and/or diarrhoea in livestock. A cross-sectional survey was conducted in Kajiado County to determine prevalence, seasonality, and molecular detection of thermophilic Campylobacter species (with emphasis on C. jejuni, C. coli, and other thermophilic Campylobacter species) in chicken, cattle, and respective pooled drinking water. A total of 457 samples comprising 265 cattle rectal swabs, 142 chicken cloacal swabs, and 50 trough water samples were collected from 55 randomly selected smallholder farms. Individual samples were subjected to standard techniques for isolation and biochemical tests, followed by singleplex polymerase chain reaction (sPCR) assays for identification and confirmation of genus and species. Overall, thermophilic Campylobacter prevalence was 35.4% (95% confidence interval (95% CI) = 31.0–39.8), with C. jejuni dominating at 55.6% (95% CI = 47.9–63.3%) over C. coli in all sample types. The highest thermophilic Campylobacter prevalence was observed in cloacal swabs of live chicken at 44.4% (95% CI = 36.2–52.6%), followed by rectal swabs from live cattle at 30.9% (95% CI = 25.3–36.5%). Water samples from cattle drinkers/trough were found to be contaminated at 34% (95% CI = 20.9–47.1%). The isolation rate was higher in cattle under the confinement system (44.3%) (95% CI = 36.1–52.5%) than in those under the free-roaming grazing system. Thermophilic Campylobacter species were isolated in both seasons, with higher prevalence (39.8% (95% CI = 33.6–45.9)) recorded during rainy and cold season in all sample types except for water. There was significant ( P < 0.05 ) association between season and thermophilic Campylobacter occurrence, even though there were no statistical differences in the prevalence values across the two seasons. Results of this study demonstrate that cattle, chicken, and respective drinking water harbour potentially pathogenic thermophilic campylobacters, with C. jejuni being widely distributed among farms. It is possible that seasonal variations and cattle confinement result in differences in thermophilic Campylobacter carriage. Further epidemiological and phylogenetic studies comparing distribution of thermophilic Campylobacter spp. isolates in livestock, environmental, and human samples are recommended to establish source attribution to reduce the impact of resultant diseases for the wellbeing of public and livestock.

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“…Members of the Campylobacter genus are nutritionally fastidious and strictly grow under anaerobic or microaerobic conditions, and they are morphologically diverse, including spiral-, curved-, or rod-shaped. Campylobacter naturally colonizes humans, other mammals, birds, reptiles, and shellfish, particularly birds, and can be transmitted to humans through contaminated food or water [ 1 , 2 , 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Phenotypic and Genomic Characteristics of Campylobacter gastrosuis sp. nov. Isolated from the Stomachs of Pigs in Beijing

Wang,

Gu,

et al. 2023

Microorganisms

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Campylobacter is among the four main causes of gastroenteritis worldwide. Most reported Campylobacter infections are caused by C. jejuni and C. coli. However, other emerging Campylobacter pathogens have been recognized as important pathogens in humans and animals. A novel bacterial strain, PS10T, was isolated from the gastric mucous of pigs in 2022 in Beijing, China. The cell was Gram-negative, microaerobic, motile, and negative for catalase, oxidase, and urease. Phylogenetic and phylogenomic analyses based on the 16S rRNA gene and core genome indicated that this isolate belongs to the genus Campylobacter. There were low dDDH relatedness and ANI values shared within this strain and its closest species C. mucosalis below the cut-off values generally recognized for isolates of the same species. The draft genome size of PS10T is 2,240,910 bp in length with a percentage of DNA G+C contents of 37.72%. Comparing the phenotypic and phylogenetic features among this isolate and its related organisms, strain PS10T represents a novel species within the genus Campylobacter, for which the name Campylobacter gastrosuis sp. nov. (Type strain PS10T = GDMCC 1.3686T = JCM 35849T) is proposed.

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Overview of methodologies for the culturing, recovery and detection of Campylobacter

Cited by 9 publications

References 87 publications

Campylobacter spp. Prevalence in Santiago, Chile: A Study Based on Molecular Detection in Clinical Stool Samples from 2014 to 2019

Campylobacter spp. Prevalence in Santiago, Chile: A Study Based on Molecular Detection in Clinical Stool Samples from 2014 to 2019

Seasonal Prevalence and Molecular Identification of Thermophilic Campylobacter from Chicken, Cattle, and Respective Drinking Water in Kajiado County, Kenya

Phenotypic and Genomic Characteristics of Campylobacter gastrosuis sp. nov. Isolated from the Stomachs of Pigs in Beijing

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