Overview of Cell Signaling Pathways in Cancer

Harvey, Amanda

doi:10.1007/978-3-319-95228-4_12

Cited by 12 publications

(10 citation statements)

References 14 publications

(17 reference statements)

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“…We identified 12 important pathways that might have an essential role in the incidence and development of LUAD: pathways in cancer, ubiquitin-mediated proteolysis, protein processing in the endoplasmic reticulum, necroptosis, hypertrophic cardiomyopathy, cardiac muscle contraction, focal adhesion, the PI3K–Akt and Ras signaling pathways, the neurotrophin and mTOR signaling pathways, and the MAPK signaling pathway. Many studies have reported that the PI3K–Akt signaling pathway, the Ras signaling pathway, the neurotrophic-signaling pathway, the mTOR-signaling pathway, and the MAPK signaling pathway are regulated in the development of lung adenocarcinoma patients [ 58 , 59 ]. The PI3K/AKT/mTOR signal pathway is a key intracellular signal transduction pathway, with an essential function in cell proliferation, growth, survival, vesicle trafficking, glucose transport, and cytoskeletal organization.…”

Section: Discussionmentioning

confidence: 99%

Circulating miR-1246 Targeting UBE2C, TNNI3, TRAIP, UCHL1 Genes and Key Pathways as a Potential Biomarker for Lung Adenocarcinoma: Integrated Biological Network Analysis

Huang

Wei

Kaliamurthi

et al. 2020

JPM

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Analysis of circulating miRNAs (cmiRNAs) before surgical operation (BSO) and after the surgical operation (ASO) has been informative for lung adenocarcinoma (LUAD) diagnosis, progression, and outcomes of treatment. Thus, we performed a biological network analysis to identify the potential target genes (PTGs) of the overexpressed cmiRNA signatures from LUAD samples that had undergone surgical therapy. Differential expression (DE) analysis of microarray datasets, including cmiRNAs (GSE137140) and cmRNAs (GSE69732), was conducted using the Limma package. cmiR-1246 was predicted as a significantly upregulated cmiRNA of LUAD samples BSO and ASO. Then, 9802 miR-1246 target genes (TGs) were predicted using 12 TG prediction platforms (MiRWalk, miRDB, and TargetScan). Briefly, 425 highly expressed overlapping miRNA-1246 TGs were observed between the prediction platform and the cmiRNA dataset. ClueGO predicted cell projection morphogenesis, chemosensory behavior, and glycosaminoglycan binding, and the PI3K–Akt signaling pathways were enriched metabolic interactions regulating miRNA-1245 overlapping TGs in LUAD. Using 425 overlapping miR-1246 TGs, a protein–protein interaction network was constructed. Then, 12 PTGs of three different Walktrap modules were identified; among them, ubiquitin-conjugating enzyme E2C (UBE2C), troponin T1(TNNT1), T-cell receptor alpha locus interacting protein (TRAIP), and ubiquitin c-terminal hydrolase L1(UCHL1) were positively correlated with miR-1246, and the high expression of these genes was associated with better overall survival of LUAD. We conclude that PTGs of cmiRNA-1246 and key pathways, namely, ubiquitin-mediated proteolysis, glycosaminoglycan binding, the DNA metabolic process, and the PI3K–Akt–mTOR signaling pathway, the neurotrophin and cardiomyopathy signaling pathway, and the MAPK signaling pathway provide new insights on a noninvasive prognostic biomarker for LUAD.

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Section: Discussionmentioning

confidence: 99%

Circulating miR-1246 Targeting UBE2C, TNNI3, TRAIP, UCHL1 Genes and Key Pathways as a Potential Biomarker for Lung Adenocarcinoma: Integrated Biological Network Analysis

Huang

Wei

Kaliamurthi

et al. 2020

JPM

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“…In the study of tumor processes, special attention is paid to signaling pathways that are responsible for the aberrant cell growth, survival, genome maintenance, etc. [ 155 ]. Signalome study provides another level for deciphering pathological processes and uses it to inform new treatments for precision medicine in cancer [ 156 ].…”

Section: Comparison Of Hepg2 Normal Hepatocyte Hb and Hccmentioning

confidence: 99%

The Curious Case of the HepG2 Cell Line: 40 Years of Expertise

Arzumanian

Kiseleva

Poverennaya

2021

IJMS

152

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Liver cancer is the third leading cause of cancer death worldwide. Representing such a dramatic impact on our lives, liver cancer is a significant public health concern. Sustainable and reliable methods for preventing and treating liver cancer require fundamental research on its molecular mechanisms. Cell lines are treated as in vitro equivalents of tumor tissues, making them a must-have for basic research on the nature of cancer. According to recent discoveries, certified cell lines retain most genetic properties of the original tumor and mimic its microenvironment. On the other hand, modern technologies allowing the deepest level of detail in omics landscapes have shown significant differences even between samples of the same cell line due to cross- and mycoplasma infection. This and other observations suggest that, in some cases, cell cultures are not suitable as cancer models, with limited predictive value for the effectiveness of new treatments. HepG2 is a popular hepatic cell line. It is used in a wide range of studies, from the oncogenesis to the cytotoxicity of substances on the liver. In this regard, we set out to collect up-to-date information on the HepG2 cell line to assess whether the level of heterogeneity of the cell line allows in vitro biomedical studies as a model with guaranteed production and quality.

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“…Hypoxia-inducible factor signalling pathway (HIF-signalling) is described for different tissues such as brain 1 , 2 , lungs 3 , 4 , cardio-vascular system 5 , 6 , muscles 7 – 9 or skin 10 – 14 . In the given tissues, HIF-signalling has a different priority compared to the rest of the affected growth, metabolic and transcription pathways 15 – 18 . In the case of malignant melanoma (MM) the HIF-signalling pathway is one of the most dominant pathways 11 , 19 , 20 .…”

Section: Introductionmentioning

confidence: 99%

Effect of hypoxia factors gene silencing on ROS production and metabolic status of A375 malignant melanoma cells

Špaková

Rabajdová

Mičková

et al. 2021

Sci Rep

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The innate response of melanocytes to exogenous or endogenous stress stimuli like extreme pH and temperature, metabolite and oxygen deficiency or a high UV dose initiates a cellular stress response. This process activates adaptive processes to minimize the negative impact of the stressor on the pigment cell. Under physiological conditions, a non-cancer cell is directed to apoptosis if the stressor persists. However, malignant melanoma cells will survive persistent stress thanks to distinct "cancerous" signaling pathways (e.g. MEK) and transcription factors that regulate the expression of so-called "survival genes" (e.g. HIF, MITF). In this survival response of cancer cells, MEK pathway directs melanoma cells to deregulate mitochondrial metabolism, to accumulate reduced species (NADH), and to centralize metabolism in the cytosol. The aim of this work was to study the effect of gene silencing in malignant melanoma A375 cells on metabolic processes in cytosol and mitochondria. Gene silencing of HIF-1α, and miR-210 in normoxia and pseudohypoxia, and analysis of its effect on MITF-M, and PDHA1 expression. Detection of cytosolic NADH by Peredox-mCherry Assay. Detection of OCR, and ECAR using Seahorse XF96. Measurement of produced O2•− with MitoTracker Red CMXRos. 1H NMR analysis of metabolites present in cell suspension, and medium. By gene silencing of HIF-1α and miR-210 the expression of PDHA1 was upregulated while that of MITF-M was downregulated, yielding acceleration of mitochondrial respiratory activity and thus elimination of ROS. Hence, we detected a significantly reduced A375 cell viability, an increase in alanine, inositol, nucleotides, and other metabolites that together define apoptosis. Based on the results of measurements of mitochondrial resipiratory activity, ROS production, and changes in the metabolites obtained in cells under the observed conditions, we concluded that silencing of HIF-1α and miR-210 yields apoptosis and, ultimately, apoptotic cell death in A375 melanoma cells.

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Overview of Cell Signaling Pathways in Cancer

Cited by 12 publications

References 14 publications

Circulating miR-1246 Targeting UBE2C, TNNI3, TRAIP, UCHL1 Genes and Key Pathways as a Potential Biomarker for Lung Adenocarcinoma: Integrated Biological Network Analysis

Circulating miR-1246 Targeting UBE2C, TNNI3, TRAIP, UCHL1 Genes and Key Pathways as a Potential Biomarker for Lung Adenocarcinoma: Integrated Biological Network Analysis

The Curious Case of the HepG2 Cell Line: 40 Years of Expertise

Effect of hypoxia factors gene silencing on ROS production and metabolic status of A375 malignant melanoma cells

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