Overview of a Workshop on Screening Methods for Detecting Potential (Anti-) Estrogenic/Androgenic Chemicals in Wildlife

Ankley, Gerald T.; Mihaich, Ellen; Stahl, R.; Tillitt, Donald E.; Colborn, Theo; McMaster, Suzzanne; Miller, Ron; Bantle, John P.; Campbell, Pamela; Denslow, Nancy D.; Dickerson, Richard E.; Folmar, Leroy C.; Fry, Michael; Giesy, John P.; Gray, L. Earl; Guiney, Patrick; Hutchinson, Thomas H.; Kennedy, Seán; Kramer, Vincent J.; LeBlanc, Gerald A.; Mayes, Monte A.; Nimrod, Alison C.; Patiño, Reynaldo; Peterson, Richard E.; Purdy, Richard; Ringer, Robert K.; Thomas, P. C.; Touart, Les; Kraak, Glen Van Der; Zacharewski, Tim

doi:10.1897/1551-5028(1998)017<0068:ooawos>2.3.co;2

Cited by 78 publications

(88 citation statements)

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“…Biological tools are needed to measure effects, which are integrated responses to all estrogenic chemicals present, including those that might be missed by chemical analysis. Hence, in vitro testing of environmental samples and chemicals has become an important complementary technique to classical chemical analysis [25,26,27,28]. It allows targeting known and unknown chemicals and mixtures of chemicals that cause specific effects at the cellular or molecular level, and it can be used for high-throughput screening.…”

Section: Introductionmentioning

confidence: 99%

Combined biological and chemical assessment of estrogenic activities in wastewater treatment plant effluents

Aerni

Kobler

Rutishauser

et al. 2004

Analytical and Bioanalytical Chemistry

211

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Five wastewater treatment plant effluents were analyzed for known endocrine disrupters and estrogenicity. Estrogenicity was determined by using the yeast estrogen screen (YES) and by measuring the blood plasma vitellogenin (VTG) concentrations in exposed male rainbow trout (Oncorhynchus mykiss). While all wastewater treatment plant effluents contained measurable concentrations of estrogens and gave a positive response with the YES, only at two sites did the male fish have significantly increased VTG blood plasma concentrations after the exposure, compared to pre-exposure concentrations. Estrone (E1) concentrations ranged up to 51 ng L(-1), estradiol (E2) up to 6 ng L(-1), and ethinylestradiol (EE2) up to 2 ng L(-1) in the 90 samples analyzed. Alkylphenols, alkylphenolmonoethoxylates and alkylphenoldiethoxylates, even though found at microg L(-1) concentrations in effluents from wastewater treatment plants with a significant industrial content, did not contribute much to the overall estrogenicity of the samples taken due to their low relative potency. Expected estrogenicities were calculated from the chemical data for each sample by using the principle of concentration additivity and relative potencies of the various chemicals as determined with the yeast estrogen screen. Measured and calculated estradiol equivalents gave the same order of magnitude and correlated rather well (R(2)=0.6).

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Section: Introductionmentioning

confidence: 99%

Combined biological and chemical assessment of estrogenic activities in wastewater treatment plant effluents

Aerni

Kobler

Rutishauser

et al. 2004

Analytical and Bioanalytical Chemistry

211

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“…Observation of the alteration of serum levels of sex steroids in fish may act as a useful method to screen the EDs in aquatic environment and may be a sensitive index to evaluate the functional damage of reproduction in fish (Ankley et al 1998). The concentrations of both testosterone and 17b-estradiol in the serum of crucian carp after 60 days exposure to alachlor were measured, and significant changes in sex hormones were observed.…”

Section: Resultsmentioning

confidence: 99%

Altered Serum Levels of Sex Steroids and Biotransformation Enzyme Activities by Long-term Alachlor Exposure in Crucian Carp (Carassius auratus)

Liu

et al. 2007

Bull Environ Contam Toxicol

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“…The proposed method makes it possible to conduct analysis in several hours instead of several days, as is required for some systems based on transformed cells of homeotherms [5,12,13]. In addition, unlike the techniques based on competitive binding of ligands with estrogen receptors, in which the affinity of the chemical compound for the receptor is determined [14], this method does not require radioactive isotopes to be used. Thus, the created two-hybrid test system is a convenient model for screening and determining estrogenic activity of samples containing individual compounds and complex mixtures as well.…”

Section: Biochemistry Biophysics and Molecular Biologymentioning

confidence: 99%

Two-hybrid yeast test system for assessment of estrogenic activity of chemical compounds

Kozhemyako

Kovalchuk

Rasskazov

et al. 2005

Dokl Biochem Biophys

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The search for natural and synthetic compounds exhibiting estrogenic and antiestrogenic activity is a topical problem of modern biology and medicine. Compounds exhibiting estrogenic activity are widely used in medical practice to correct functioning and to treat a number of endocrine disorders and sexual diseases. Antagonists of steroid hormones may be used for designing antitumor preparation to treat hormonedependent malignancies. In addition, the search for compounds that display estrogenic activity and the determination of their concentrations in various water bodies and soil are important for assessing environmental pollution with mimetics of steroid hormones, which affect metabolic pathways and mimic or modify the effect of estrogens and, as a result, can disturb the functions of the endocrine system and pose a threat to health of humans and animals.It is known that the main target for estrogen and estrogen-like compounds is the nuclear steroid receptor (ER receptor), which binds in a specific manner with the steroid hormone and regulates gene expression [1]. In human and homeothermic animals, inactivated ER receptors exist in large complexes, being associated with heat shock proteins. After estrogen binds with the ER receptor, heat shock proteins dissociate, thereby triggering a conformational change that activates the receptor and causes its dimerization. The homodimeric complex displays a high affinity for the specific DNA sequence located in the regulatory region of estrogeninducible genes. Binding with this region initiates the biosynthesis of transcriptional factors that, in turn, activate the target gene expression. Then, mRNA is translated to yield protein that are the final effectors of cell responses observed. By inducing the synthesis of new proteins affecting cell functions, estrogens highly effectively influence various biochemical and physiological processes. Xenoestrogens or mimetics may function as ER ligands and bind with receptors, thus modulating endocrine metabolic pathways via the receptor-mediated process.One of the key problems in search for and study of compounds exhibiting steroid hormone activity is designing new test systems for rapid, highly sensitive, and highly selective assessment of the hormonal activity of preparations of interest. This study was devoted to designing a two-hybrid yeast test system containing human estrogen receptors and β -galactosidase gene as a reporter enzyme. For this purpose, we used the plasmids pCL1, pGBKT7-Lam (Clontech, United States); two expression plasmids ( pGBT9-hER α , containing the ER α human estrogen receptor gene, and pGAD424-hTIF2, containing the TIF2 expression coactivator gene) were kindly provided by Dr. Nishihara Tsutomu (Department of Environmental Bioscience, Osaka University, Japan). The Saccharomyces cerevisiae strain Y187 ( MAT α , 112 , gal4 ∆ , met -, gal80 ∆ , URA3::GAL1 UAS -GAL1 TATA -lacZ ) from Clontech (United States) was used as yeast culture. Yeast cells were transformed with plasmids using the lithium-acetate method; t...

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Overview of a Workshop on Screening Methods for Detecting Potential (Anti-) Estrogenic/Androgenic Chemicals in Wildlife

Cited by 78 publications

References 0 publications

Combined biological and chemical assessment of estrogenic activities in wastewater treatment plant effluents

Combined biological and chemical assessment of estrogenic activities in wastewater treatment plant effluents

Altered Serum Levels of Sex Steroids and Biotransformation Enzyme Activities by Long-term Alachlor Exposure in Crucian Carp (Carassius auratus)

Two-hybrid yeast test system for assessment of estrogenic activity of chemical compounds

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