Overview, consequences, and strategies for overcoming matrix effects in LC-MS analysis: a critical review

Nasiri, Azadeh; Jahani, Reza; Mokhtari, Shaya; Yazdanpanah, Hassan; Daraei, Bahram; Faizi, Mehrdad; Kobarfard, Farzad

doi:10.1039/d1an01047f

Cited by 68 publications

(31 citation statements)

References 111 publications

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“…Recovery and matrix effects were assessed by a postextraction addition approach at low, medium, and high QC levels by using 6 lots of blank samples. 11 The IS-normalized matrix factor (IS-norm MF) was calculated by dividing the MF of gilteritinib by the MF of the IS. The CV% of the IS-norm MF calculated from the 6 lots should be <15%.…”

Section: Methodsmentioning

confidence: 99%

Development and Validation of an LC-MS/MS Method to Quantify Gilteritinib and Its Clinical Application in Patients With FLT3 Mutation–Positive Acute Myelogenous Leukemia

Zhang

Tajima

Suetsugu

et al. 2022

Therapeutic Drug Monitoring

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Background: Gilteritinib, a novel oral tyrosine kinase inhibitor, is used to treat acute myeloid leukemia (AML) with FMS-like tyrosine kinase-3 (FLT3) mutations. Therapeutic drug monitoring (TDM) of gilteritinib is important for improving clinical outcomes and ensuring safety. Therefore, this study aimed to develop a simplified method for quantifying gilteritinib in human plasma using liquid chromatography-tandem mass spectrometry. Methods: Liquid chromatography was performed by using anAcquity BEH C18 column (50 mm • 2.1 mm, 1.7 mm) and a gradient elution with 0.1% formic acid in water (A) and acetonitrile (B). Detection was performed by using a Shimadzu tandem mass spectrometer through multiple reaction monitoring in the positive-ion mode. Results:The developed method enabled quantification of gilteritinib in 4 minutes and was validated by evaluating selectivity, calibration curve (10-1000 ng/mL, r 2 . 0.99), a lower limit of quantification (LLOQ), accuracy (overall bias 24.2% to 1.9%), precision (intraday CV # 7.9%; interday CV # 13.6%), carryover, recovery, matrix effect, dilution integrity, and stability according to the US Food and Drug Administration (FDA) guidelines. This method was successfully applied to the TDM of gilteritinib trough concentrations in 3 patients with AML. Conclusions:The developed method fulfilled the FDA guideline criteria and can easily be implemented to facilitate TDM in patients receiving gilteritinib in a clinical setting.

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Section: Methodsmentioning

confidence: 99%

Development and Validation of an LC-MS/MS Method to Quantify Gilteritinib and Its Clinical Application in Patients With FLT3 Mutation–Positive Acute Myelogenous Leukemia

Zhang

Tajima

Suetsugu

et al. 2022

Therapeutic Drug Monitoring

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“…21 Electrospray ionization (ESI) is the most used interface in today's LC-MS hardware. 21 However, it shows higher susceptibility with respect to other atmospheric pressure ionization (API) sources. 21,22 To assess the order of the matrix effect, postcolumn infusion and post-extraction addition are the methods of choice, with the latter being widely used for method validation and for this study.…”

Section: Introductionmentioning

confidence: 99%

“…21 However, it shows higher susceptibility with respect to other atmospheric pressure ionization (API) sources. 21,22 To assess the order of the matrix effect, postcolumn infusion and post-extraction addition are the methods of choice, with the latter being widely used for method validation and for this study. 21,22 In addition to this, there is also the possibility of solvent dilution and minimizing the injection volume to reduce the matrix effect.…”

Section: Introductionmentioning

confidence: 99%

“…21,22 To assess the order of the matrix effect, postcolumn infusion and post-extraction addition are the methods of choice, with the latter being widely used for method validation and for this study. 21,22 In addition to this, there is also the possibility of solvent dilution and minimizing the injection volume to reduce the matrix effect. 21,22 However, this can lead to problems in terms of sensitivity.…”

Section: Introductionmentioning

confidence: 99%

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Sulfonated halloysite nanotubes as a novel cation exchange material for solid phase extraction of toxic pyrrolizidine alkaloids

et al. 2022

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“…The multiple reaction monitoring approaches can well eliminate intruders and make the analysis easier. 2 …”

Section: Introductionmentioning

confidence: 99%

Challenges for the determination of spiramycin in aqueous matrices using LC-MS/MS: evidence for the solvent intrusion on the molecule integrity

et al. 2022

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Overview, consequences, and strategies for overcoming matrix effects in LC-MS analysis: a critical review

Abstract: The high-performance ‎liquid chromatography-mass ‎spectrometry (LC-MS) ‎technique is widely applied ‎to ‎routine analysis in many ‎matrices. Despite the ‎enormous application of ‎LC/MS, this technique is ‎subjected to ‎drawbacks ‎called matrix...

Cited by 68 publications

References 111 publications

Development and Validation of an LC-MS/MS Method to Quantify Gilteritinib and Its Clinical Application in Patients With FLT3 Mutation–Positive Acute Myelogenous Leukemia

Development and Validation of an LC-MS/MS Method to Quantify Gilteritinib and Its Clinical Application in Patients With FLT3 Mutation–Positive Acute Myelogenous Leukemia

Sulfonated halloysite nanotubes as a novel cation exchange material for solid phase extraction of toxic pyrrolizidine alkaloids

Challenges for the determination of spiramycin in aqueous matrices using LC-MS/MS: evidence for the solvent intrusion on the molecule integrity

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