Overview and analysis of the polyprotein cleavage sites in the family <i>Potyviridae</i>

Adams, Michael J.; Antoniw, J. F.; Beaudoin, Frédéric

doi:10.1111/j.1364-3703.2005.00296.x

Cited by 363 publications

(266 citation statements)

References 47 publications

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“…Both Su03-07 and Su94-54 had a typical potyvirus genome organization, with a large ORF encoding a 3281 aa and 3295 aa polyprotein, respectively, with a putative frameshift in the P3 coding region yielding a P3N-PIPO protein (Chung et al, 2008). The putative cleavage sites in the polyprotein were similar to those of other potyviruses (Adams et al, 2005a). The deduced amino-acid sequence of Su03-07 and Su94-54 contained the three conserved motifs involved in aphid transmission of potyviruses (Pirone and Blanc, 1996); "DAG" was present in the N-terminal part of the CP and "PTK" or "PTR" in the HC-Pro protein.…”

Section: Complete Sequences Of Su94-54 and Su03-07mentioning

confidence: 77%

New species in the papaya ringspot virus cluster: Insights into the evolution of the PRSV lineage

Desbiez¹,

Wipf-Scheibel²,

Millot³

et al. 2017

Virus Research

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Please cite this article as: Desbiez, C., Wipf-Scheibel, C., Millot, P., Verdin, E., Dafalla, G., Lecoq, H., New species in the papaya ringspot virus cluster: insights into the evolution of the PRSV lineage.Virus Research http://dx.doi.org/10. 1016/j.virusres.2017.06.022 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. SuWMV appeared 10 times more frequent than WMVBV in that country (14.6% vs. 1.5% of the samples tested). The geographic structure and molecular diversity patterns of the putative and acknowledged species suggest that the PRSV-like cluster originated in the Old World about 3600 years ago, with an important diversification in Africa.

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Section: Complete Sequences Of Su94-54 and Su03-07mentioning

confidence: 77%

New species in the papaya ringspot virus cluster: Insights into the evolution of the PRSV lineage

Desbiez¹,

Wipf-Scheibel²,

Millot³

et al. 2017

Virus Research

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“…En los potyvirus, como SCMV, en el extremo 5´UTR, en lugar de una estructura cap, se encuentra una proteína viral VPg unida covalentemente que remplaza la estructura cap, y el extremo 3´UTR está poliadelinado [26]. De acuerdo a los resultados de entrecruzamiento por luz ultravioleta, en este estudio se encontró que en el hospedante maíz existe por lo menos una proteína de aproximadamente 53 kDa, que posiblemente esté interactuando con la región 5´ UTR del potyvirus SCMV.…”

Section: Resultados Y Análisisunclassified

Exploración de la interacción entre la región 5´UTR del Sugarcane Mosaic Virus (SCMV) y proteínas del hospedero maíz

Chaves-Bedoya

2017

Respuestas

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Resumen Antecedentes: El virus del mosaico de la caña de azúcar, SCMV (Potyviridae) es un miembro de la gran familia de virus de ARN de cadena positiva, sin una estructura CAP en su extremo 5´no traducido (5`UTR), pero con una proteína viral unida al genoma (VPg) y una cola poli A en su extremo 3´UTR. Se ha sugerido que proteínas del hospedero hacen un puente entre las regiones no traducidas virales 5´y 3´ para potenciar la traducción viral. Dado que las regiones no traducidas presentes en los genomas virales contienen elementos involucrados en la regulación de su ciclo replicativo, es importante analizar la interacción entre estas regiones y las proteínas virales o del hospedero para inferir su función. Objetivo: Determinar si existen proteínas del hospedero maíz que pudieran estar interactuando con la región 5´UTR de SCMV. Metodología: La región 5´UTR de SCMV se amplificó por PCR incluyendo la secuencia del promotor T7 en la secuencia del oligo 5´ UTR y se generaron sus transcritos marcados radiactivamente. Los transcritos marcados fueron entrecruzados con extractos proteicos de hojas de maíz sanas e infectadas. Resultados: Los resultados sugieren la presencia de una proteína putativa de interacción del hospedante maíz de aproximadamente 53kDa, con la región 5´UTR de SCMV. Conclusión: Los ensayos de entrecruzamiento, especificidad y estabilidad de los complejos ARN-proteína sugieren que en el hospedante maíz existe al menos una proteína que interactúa con la región 5´ UTR de SCMV.Palabras Clave: Complejo ARN-proteína, entrecruzamiento UV, fracción proteica, Potyvirus, VPg.Abstract Background: Sugarcane mosaic virus is a member of the great family of positive sense RNA viruses, without a CAP structure in its 5´UTR end, but with a viral protein attached to the genome (VPg) and a poli A tail in its 3´UTR end. It has been suggested that some host proteins make a bridge between the untranslated 5´and 3´ regions (UTRs) to enhance the canonical and/or non-canonical virus translation. Since the UTR regions present in the viral genomes have some elements involved in the regulation of their replicative cycle, it is important to analyze the interaction that may occur between these regions and the viral or host proteins to infer regarding its function. Objective: To identify proteins of the host maize that could be interacting with the 5´UTR region of SCMV. Methodology: 5´UTR region of SCMV was amplified by PCR including the sequence of the T7 promoter in the 5´oligo sequence. The transcripts were radioactively labeled. Labeled transcripts were crosslinked with proteins extracts from healthy and infected maize leaves. Results: The results suggest the presence of a 53 kDa putative protein interacting with the 5 ´UTR region of SCMV. Conclusion: Crosslinking essays, specificity and the stability of the RNA-protein complex suggest that in maize host there is at least one protein that interacts with the 5´UTR region of SCMV.Keywords: RNA-Protein complex, UV crosslinking, protein fraction, Potyvirus, VPg

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“…Th e fi rst nuclear inclusion protein, NIa, possesses two domains: VPg, a viral genome-linked protein, and NIaPro, a protease responsible for cleavage of most proteins of the precursor polyprotein (Adams et al, 2005). The viral genome-linked protein VPg is a protein involved in Fig.…”

Section: Genome Sequence Propertiesmentioning

confidence: 99%

“…Proteinase P1 comprises 289 residues (32.47 kDa) and is cleaved at the C-terminal end at sequence MVQF/S, which is identical in PVV isolates DV42, and KER.LAL.P (Adams et al, 2005). P1 is the least conserved protein in PVV-phureja sharing 70-77% amino acid identity with homologs within the PVV group in agreement with previous observations, which showed P1 to be the protein most variable in size and sequence within the genus Potyvirus (Rohozková and Navrátil, 2011).…”

Section: Genome Sequence Propertiesmentioning

confidence: 99%

Genome sequence of a divergent Colombian isolate of potato virus V (PVV) infecting Solanum phureja

Gutiérrez¹,

Hj²,

M³

2016

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Summary. -Deep sequencing analysis of the transcriptome of a Solanum phureja cv. Criolla Colombia plant with symptoms typical of a virus disease revealed an infection with potato virus V (PVV). Th e PVV-phureja genome comprises 9904 nt, exhibits 83% nucleotide identity with currently fully sequenced PVV isolates and contains one large ORF that codes for a polyprotein of 3065 residues fl anked by 5' and 3' UTR of 217 and 448 nt, respectively. Phylogenetic analysis of the PVV-phureja polyprotein indicates that it is divergent with respect to most PVV isolates. Th is is the fi rst complete PVV genome of an isolate infecting a host diff erent to S. tuberosum and, to this date, the only one from the South American Andes.

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Overview and analysis of the polyprotein cleavage sites in the family Potyviridae

Cited by 363 publications

References 47 publications

New species in the papaya ringspot virus cluster: Insights into the evolution of the PRSV lineage

New species in the papaya ringspot virus cluster: Insights into the evolution of the PRSV lineage

Exploración de la interacción entre la región 5´UTR del Sugarcane Mosaic Virus (SCMV) y proteínas del hospedero maíz

Genome sequence of a divergent Colombian isolate of potato virus V (PVV) infecting Solanum phureja

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