Overproduction of PU.1 in mast cell progenitors: its effect on monocyte- and mast cell-specific gene expression

Nishiyama, Chiharu; Nishiyama, Makoto; Ito, Toshifumi; Masaki, Shigehiro; Maeda, Keiko; Masuoka, Nobutaka; Yamane, Hisakazu; Kitamura, Toshio; Ogawa, Hideoki; Okumura, Ko

doi:10.1016/j.bbrc.2003.11.145

Cited by 26 publications

(39 citation statements)

References 42 publications

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“…In addition, overexpression of PU.1 in CD34 ϩ human myeloid progenitors promotes LC development (10,11). In recent analysis, we found that monocyte-specific gene expression and monocyte-like morphological change were induced by overproduction of PU.1 in mouse bone marrow-derived mast cell progenitors (12,20,21). In this study, overproduction of PU.1 caused several monocyte-like characteristics on both BMMC and PMC, suggesting that developed mast cells still have the capacity to exhibit monocyte-like features.…”

Section: Discussionmentioning

confidence: 94%

“…Infection of BMMC and PMC was performed, according to a previously reported method (12,20). In brief, pMX-puro (mock vector) and pMXpuro-PU.1 (for the expression of wild-type PU.1) were transiently introduced into Plat-E with Fugene6 (Roche Diagnostics).…”

Section: Transfectionmentioning

confidence: 99%

“…Electron microscopy and May-Grünwald-Giemsa's staining were performed, as described previously (12).…”

Section: Morphological Analysismentioning

confidence: 99%

“…Recently, we found that overproduction of PU.1 in mouse bone marrow-derived hemopoietic progenitor cells developing toward mast cells induced the expression of several monocyte-specific genes and caused morphological change (12). In this study, we conducted overexpression of PU.1 by retrovirus transfection system in differentiated mast cells, mouse bone marrow-derived mast cells (BMMC), and peritoneal mast cells (PMC), and examined its effect on the expression of monocyte-and mast cell-specific markers and the cell morphology.…”

mentioning

confidence: 99%

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Mast Cells Acquire Monocyte-Specific Gene Expression and Monocyte-Like Morphology by Overproduction of PU.1

Ito

Nishiyama²,

Nishiyama

et al. 2005

The Journal of Immunology

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PU.1 is a myeloid- and lymphoid-specific transcription factor that belongs to the Ets family. Recently, we found that overproduction of PU.1 in mouse bone marrow-derived hemopoietic progenitor cells induced monocyte-specific gene expression and caused their monocyte-like morphological change. In the present study, PU.1 was overproduced by using retrovirus expression system in differentiated bone marrow-derived mast cells. By overexpression of PU.1, cell surface expression of MHC class II, CD11b, CD11c, and F4/80 was induced, accompanied by reduced expression of c-kit, a mast cell-specific marker. Morphology of PU.1-transfected cells was altered toward monocyte-like one. PU.1-overproducing cells acquired T cell stimulatory ability and showed an increase in response to LPS stimulation, while response through FcεRI was markedly reduced by overproduction of PU.1. These results suggest that the differentiated mast cells still have potential to display monocytic features. When PU.1 was overproduced in a different type of mast cell, peritoneal mast cells, similar monocyte-like morphological change, and the expression of CD11b and F4/80 were induced. However, surface level of CD11c and MHC class II was not affected. These results indicate that the potential capacity to exhibit monocytic features is different between both the mast cells.

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Section: Discussionmentioning

confidence: 94%

Section: Transfectionmentioning

confidence: 99%

“…Electron microscopy and May-Grünwald-Giemsa's staining were performed, as described previously (12).…”

Section: Morphological Analysismentioning

confidence: 99%

mentioning

confidence: 99%

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Mast Cells Acquire Monocyte-Specific Gene Expression and Monocyte-Like Morphology by Overproduction of PU.1

Ito

Nishiyama²,

Nishiyama

et al. 2005

The Journal of Immunology

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“…Low levels of PU.1 expression promote B-cell lymphocyte development, while enforced expression of high levels of PU.1 drives macrophage differentiation at the expense of B-cells (47). In support of this notion, retroviral expression of PU.1 into murine hematopoietic cells from marrow promotes monocyte development in culture conditions that usually support mast cell differentiation (48). Furthermore, enforced expression of PU.1 into differentiated marrow derived mast cells will give rise to cells with the morphologic appearance and the gene expression profile characteristic of monocytes (49).…”

Section: Pu1mentioning

confidence: 91%

Mast cell transcriptional networks

Takemoto

Lee

Jegga

et al. 2008

Blood Cells, Molecules, and Diseases

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Unregulated activation of mast cells can contribute to the pathogenesis of inflammatory and allergic diseases, including asthma, rheumatoid arthritis, inflammatory bowel disease, and multiple sclerosis (1;2). Absence of mast cells in animal models can lead to impairment in the innate immune response to parasites and bacterial infections (3)(4)(5). Aberrant clonal accumulation and proliferation of mast cells can result in a variety of diseases ranging from benign cutaneous mastocytosis to systemic mastocytosis or mast cell leukemia(6). Understanding mast cell differentiation provides important insights into mechanisms of lineage selection during hematopoiesis and can provide targets for new drug development to treat mast cell disorders,. In this review, we discuss controversies related to development, sites of origin,, and the transcriptional program of mast cells. KeywordsMast cells; transcription factors; GATA; PU.1; Mitf Origins of Mast CellsLike other granulocytes (neutrophils, eosinophils and basophils), mast cells are derived from hematopoietic stem cells in the marrow; however, they are unique with regard to the tissue compartments in which they traffic and reside. The gastrointestinal mucosa is the major compartment occupied by committed mast cell precursors in the mouse (7). Trafficking to these compartments is dependent on the expression of the alpha4 beta7 integrin and the chemokine receptor CXCR2.(8;9) Mast cell progenitors migrate to the connective tissue of the skin, lung, and the mucosa of other gastrointestinal tissues where they mature under the influence local Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Transplantation studies in mice indicate that mast cells are derived from hematopoietic stem cells of marrow (or spleen)(10;11). Studies using prospectively identified hematopoietic precursors have reported different precursor cells that give rise to the committed mast cell progenitor One study found that the committed mast cell progenitor (MCP) arose from an early multipotential progenitor (MPP) that is distinct from the common myeloid progenitor (12). However, using a similar strategy to prospectively isolate hematopoietic precursors from mouse marrow, another study found that the MCP was derived from either the common myeloid progenitor or the granulocyte-monocyte progenitor(13) (Figure 1). Plasticity may permit other committed progenitor cell populations to be "reprogrammed" into the mast cell lineage by altering the expression of selected transcription factors. Isolated common lymphoid precursors that are retrovirally-tranduced with the tr...

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The transcription factor profile of human mast cells in comparison with monocytes and granulocytes

Babina

Schülke

Kirchhof

et al. 2005

CMLS, Cell. Mol. Life Sci.

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Expression profiles of mRNAs and proteins for various transcription factors were determined for human skin mast cells (sMCs), leukemic HMC-1 MCs, monocytes and granulocytes. By quantitative RT-PCR, sMCs expressed lower levels of c-fos, PU.1, C/EBPalpha, and C/EBPepsilon than monocytes and granulocytes, but higher levels of MITF, SCL, GATA-1 and GATA-2. At the protein level, MITF, SCL, GATA-2, Elf-1 and c-fos were clearly detectable in sMCs. With the exception of c-fos, these proteins were absent or expressed only slightly in monocytes and granulocytes. The expression of NF-E2p45, GATA-1, PU.1, Ets-1, C/EBPalpha and C/EBPepsilon was below the detection limit in sMCs, but detectable in other myelocytes. The high expression of SCL and GATA-2 in sMCs is reminiscent of stem cells. The absence of C/EBPvarepsilon in sMCs, but strong expression in HMC-1, suggests it may impair MC maturation. In summary, mature human MCs can be characterized as C/EBPalpha(low), C/EBPvarepsilon-, PU.1(low), GATA-1(low), GATA-2+, SCL+, MITF(high).

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Overproduction of PU.1 in mast cell progenitors: its effect on monocyte- and mast cell-specific gene expression

Cited by 26 publications

References 42 publications

Mast Cells Acquire Monocyte-Specific Gene Expression and Monocyte-Like Morphology by Overproduction of PU.1

Mast Cells Acquire Monocyte-Specific Gene Expression and Monocyte-Like Morphology by Overproduction of PU.1

Mast cell transcriptional networks

The transcription factor profile of human mast cells in comparison with monocytes and granulocytes

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