Overproduction of isoprenoids by Saccharomyces cerevisiae in a synthetic grape juice medium in the absence of plant genes

Camesasca, Laura; Minteguiaga, Manuel; Fariña, Laura; Salzman, Valentina; Aguilar, Pablo S.; Gaggero, Carina; Carrau, Francisco

doi:10.1016/j.ijfoodmicro.2018.05.025

Cited by 14 publications

(9 citation statements)

References 31 publications

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“…In S. cerevisiae, COQ1 overexpression can increase the production of semiterpenoids (such as farnesol and nerolid), but the specific reasons for this are unclear. 114 In addition, Camesasca et al demonstrated that COQ1 deletion reduced the amount of FPP instead of increasing it. 115 In eukaryotes, FPP is converted to heme A under the continuous catalysis of heme O synthase (COX10) and heme A synthase (COX15).…”

Section: Optimization Of Farnesene Metabolic Fluxmentioning

confidence: 99%

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Recent Advances in the Biosynthesis of Farnesene Using Metabolic Engineering

Tang

Wen

et al. 2021

J. Agric. Food Chem.

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Farnesene, as an important sesquiterpene isoprenoid polymer of acetyl-CoA, is a renewable feedstock for diesel fuel, polymers, and cosmetics. It has been widely applied in agriculture, medicine, energy, and other fields. In recent years, farnesene biosynthesis is considered a green and economical approach because of its mild reaction conditions, low environmental pollution, and sustainability. Metabolic engineering has been widely applied to construct cell factories for farnesene biosynthesis. In this paper, the research progress, common problems, and strategies of farnesene biosynthesis are reviewed. They are mainly described from the perspectives of the current status of farnesene biosynthesis in different host cells, optimization of the metabolic pathway for farnesene biosynthesis, and key enzymes for farnesene biosynthesis. Furthermore, the challenges and prospects for future farnesene biosynthesis are discussed.

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Section: Optimization Of Farnesene Metabolic Fluxmentioning

confidence: 99%

“…In S. cerevisiae, COQ1 overexpression can increase the production of semiterpenoids (such as farnesol and nerolid), but the specific reasons for this are unclear . In addition, Camesasca et al demonstrated that COQ1 deletion reduced the amount of FPP instead of increasing it .…”

Section: Optimization Of Farnesene Metabolic Fluxmentioning

confidence: 99%

Recent Advances in the Biosynthesis of Farnesene Using Metabolic Engineering

Tang

Wen

et al. 2021

J. Agric. Food Chem.

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“…Overexpression of COQ1 together with a mutated FPP synthetase, Erg20 K197E , resulted in significantly higher levels of linalool (above 750 μg/L), geraniol, α-terpineol and the sesquiterpenes, farnesol and nerolidol (Camesasca et al. 2018). Recently, a methodology was developed to create brewer's yeasts capable of biosynthesizing monoterpenes to similar levels as those typically found in finished beer, and importantly, beer brewed with this yeast was characterized by a hoppy aroma in sensory analysis, without the addition of any external hops (Denby et al.…”

Section: Biosynthesis and Occurrence Of Fruity And Floral Aroma Compomentioning

confidence: 99%

The molecular biology of fruity and floral aromas in beer and other alcoholic beverages

Holt

Mikš

Carvalho

et al. 2018

FEMS Microbiology Reviews

169

125

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Aroma compounds provide attractiveness and variety to alcoholic beverages. We discuss the molecular biology of a major subset of beer aroma volatiles, fruity and floral compounds, originating from raw materials (malt and hops), or formed by yeast during fermentation. We introduce aroma perception, describe the most aroma-active, fruity and floral compounds in fruits and their presence and origin in beer. They are classified into categories based on their functional groups and biosynthesis pathways: (1) higher alcohols and esters, (2) polyfunctional thiols, (3) lactones and furanones, and (4) terpenoids. Yeast and hops are the main sources of fruity and flowery aroma compounds in beer. For yeast, the focus is on higher alcohols and esters, and particularly the complex regulation of the alcohol acetyl transferase ATF1 gene. We discuss the release of polyfunctional thiols and monoterpenoids from cysteine- and glutathione-S-conjugated compounds and glucosides, respectively, the primary biological functions of the yeast enzymes involved, their mode of action and mechanisms of regulation that control aroma compound production. Furthermore, we discuss biochemistry and genetics of terpenoid production and formation of non-volatile precursors in Humulus lupulus (hops). Insight in these pathways provides a toolbox for creating innovative products with a diversity of pleasant aromas.

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“…Therefore, enough GPP is one of the keys to improve linalool production. Several studies have shown that mutations at K197, F96, and N127 of ERG20 can prefer to produce GPP, especially double mutations of F96W and N127W (ERG20 F96W−N127W ) showed excellent ability to increase monoterpene production [ 15 – 17 ]. To date, several strategies have been established to increase the flux of GPP to linalool synthesis [ 8 , 18 – 21 ].…”

Section: Introductionmentioning

confidence: 99%

Enhancement of linalool production in Saccharomyces cerevisiae by utilizing isopentenol utilization pathway

et al. 2022

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Background Linalool is a monoterpenoid, also a vital silvichemical with commercial applications in cosmetics, flavoring ingredients, and medicines. Regulation of mevalonate (MVA) pathway metabolic flux is a common strategy to engineer Saccharomyces cerevisiae for efficient linalool production. However, metabolic regulation of the MVA pathway is complex and involves competition for central carbon metabolism, resulting in limited contents of target metabolites. Results In this study, first, a truncated linalool synthase (t26AaLS1) from Actinidia arguta was selected for the production of linalool in S. cerevisiae. To simplify the complexity of the metabolic regulation of the MVA pathway and increase the flux of isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP), we introduced the two-step isopentenyl utilization pathway (IUP) into S. cerevisiae, which could produce large amounts of IPP/DMAPP. Further, the S. cerevisiae IDI1 (ecoding isopentenyl diphosphate delta-isomerase) and ERG20F96W−N127W (encoding farnesyl diphosphate synthase) genes were integrated into the yeast genome, combined with the strategies of copy number variation of the t26AaLS1 and ERG20F96W−N127W genes to increase the metabolic flux of the downstream IPP, as well as optimization of isoprenol and prenol concentrations, resulting in a 4.8-fold increase in the linalool titer. Eventually, under the optimization of carbon sources and Mg2+ addition, a maximum linalool titer of 142.88 mg/L was obtained in the two-phase extractive shake flask fermentation. Conclusions The results show that the efficient synthesis of linalool in S. cerevisiae could be achieved through a two-step pathway, gene expression adjustment, and optimization of culture conditions. The study may provide a valuable reference for the other monoterpenoid production in S. cerevisiae.

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Overproduction of isoprenoids by Saccharomyces cerevisiae in a synthetic grape juice medium in the absence of plant genes

Cited by 14 publications

References 31 publications

Recent Advances in the Biosynthesis of Farnesene Using Metabolic Engineering

Recent Advances in the Biosynthesis of Farnesene Using Metabolic Engineering

The molecular biology of fruity and floral aromas in beer and other alcoholic beverages

Enhancement of linalool production in Saccharomyces cerevisiae by utilizing isopentenol utilization pathway

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