Overproduction of a β-fructofuranosidase1 with a high FOS synthesis activity for efficient biosynthesis of fructooligosaccharides

Aung, Thu; Jiang, Hong; Liu, Guanglei; Chi, Zhe; Huang, Zhong; Chi, Zhen‐Ming

doi:10.1016/j.ijbiomac.2019.03.039

Cited by 31 publications

(41 citation statements)

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“…Invertase consolidated in the food industry as one of the most important carbohydrase for the sector (Lincoln et al., 2018). It presents numerous applications, such as the production of artificial honey (Nascimento et al., 2019), the synthesis of fructooligosaccharides (FOS) (Aung et al., 2019), and the development of enzymatic electrodes for the detection of sucrose in food and beverage (Park et al., 1991), in the production of invert sugar (Ozcan et al., 2019). It is also one of the most employed enzymes by the confectionery industry (Nadeem et al., 2015).…”

Section: Introductionmentioning

confidence: 99%

Cunninghamella echinulata PA3S12MM invertase: Biochemical characterization of a promiscuous enzyme

et al. 2021

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The Cunninghamella echinulata PA3S12MM fungus is a great producer of invertases in a growth medium supplemented by apple peels. The enzyme was purified 4.5 times after two chromatographic processes, and it presented a relative molecular mass of 89.2 kDa. The invertase reached maximum activity at pH of 6 and at 60°C, in addition to presenting stability in alkaline pH and thermal activation at 50°C. The enzymatic activity increased in the presence of Mn 2+ and dithiothreitol (DTT), while Cu 2+ and Z 2+ ions inhibited it. Also, DTT showed to protect enzymatic activity. The apparent values for K m , V máx , and K cat for the sucrose hydrolysis were, respectively, 173.8 mmol/L, 908.7 mmol/L min −1 , and 1,388.79 s −1 . The carbohydrate content was of 83.13%. The invertase presented hydrolytic activity over different types of glycosidic bonds, such as α1 ↔ 2β (sucrose), α1 → 4 (polygalacturonic acid), α1 → 4 and α1 → 2 (pectin), and α1 ↔ 1 (trehalose), indicating that the enzyme is multifunctional.Thus, the biochemical properties showed by the C. echinulata PA3S12MM suggest a broad industrial application, such as in the biomass hydrolysis or in the food industry.

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Section: Introductionmentioning

confidence: 99%

Cunninghamella echinulata PA3S12MM invertase: Biochemical characterization of a promiscuous enzyme

et al. 2021

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“…Effect of exogeneous nitrogen and phosphorous on transfructosylating enzyme production in shake asks Effect of NaNO 3 -N/yeast extract-N ratio NaNO 3 and yeast extract are two commonly used nitrogen sources for transfructosylating enzyme production by Aureobasidium strains. In many studies with synthetic sucrose media, these two nitrogen sources were used together at high concentrations of 10-20 g/L with the addition of other nutrients such as phosphorous, magnesium and potassium (Aung et al 2019;Jung et al 2011;Salinas and Perotti 2009;Yoshikawa et al 2006;Zhang et al 2019). Since molasses is rich in many nutrients, only exogeneous nitrogen and phosphorus were used in the present study.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, relatively high concentrations of nitrogen sources (10-20 g/L) and phosphorous sources (~ 5 g/L) together with other nutrients such as magnesium are often used in synthetic sucrose media for producing transfructosylating enzymes (Aung et al 2019;Dominguez et al 2012;Jung et al 2011;Salinas and Perotti 2009;Sangeetha et al 2004a;Sangeetha et al 2004b;Shin et al 2004;Vandáková et al 2004;Zhang et al 2019). In the studies of transfructosylating enzyme production by Aureobasidium strains, combination of yeast extract with NaNO 3 is widely used in synthetic sucrose medium (Aung et al 2019;Jung et al 2011;Salinas and Perotti 2009;Vandáková et al 2004;Yoshikawa et al 2006;Zhang et al 2019). Some studies reported the production of transfructosylating enzymes using molasses by Aspergillus strains as media but high-cost organic nitrogen sources such as yeast extract and peptone were still used at relatively high concentrations (e.g., 15-30 g/L) with others (Dorta et al 2006;Xie et al 2017).…”

Section: Effect Of Exogenous Nano 3 and Na 2 Hpomentioning

confidence: 99%

“…pullulans CCY 27-1-94 was only reported in one study, in which combination of 10 g/L yeast extract and 10 g/L NaNO 3 led to the maximum FFase activity production using synthetic sucrose media (Vandáková et al 2004). Moreover, high concentrations of yeast extract and NaNO 3 , e.g., 10-20 g/L (each) at various ratios were used for transfructosylating enzyme production from pure sucrose media by Aureobasidium strains (Aung et al 2019;Jung et al 2011;Salinas and Perotti 2009;Zhang et al 2019).…”

Section: Introductionmentioning

confidence: 99%

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Highly Efficient Production of Transfructosylating Enzymes Using Low-Cost Sugarcane Molasses by A. Pullulans FRR 5284

Khatun

Hassanpour

Harrison

et al. 2021

Preprint

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In this study, sugarcane molasses was used to produce transfructosylating enzymes by A. pullulans FRR 5284. It was found that NaNO3 was a better nitrogen source than yeast extract while exogeneous phosphorous was not needed. Adding only 4.4 g/L NaNO3 into the molasses medium containing 100 g/L sugars led to the highest total transfructosylating activity of 123.8 U/mL. Scale-up of the enzyme production process from shake flasks to 1 L reactor improved the enzyme activity and productivity to 171.7 U/mL and 3.58 U/mL/h, 39% and 108% higher than the corresponding activity and productivity from shake flasks, respectively. FOS production from 500 g/L sucrose led to the highest yields of ~ 61% using intracellular, extracellular, and total enzymes from shake flasks and the reactor. Enzymes from different sources led to very different FOS profiles, indicating that FOS profiles can be controlled by adjusting intracellular and extracellular enzyme ratios to adjust prebiotic activity.

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“…3 production of fructo-oligosaccharides at higher sucrose concentrations with high nutraceutical properties (prebiotics) (4).…”

Section: Introductionmentioning

confidence: 99%

Optimization of β-Fructofuranosidase Production from Agrowaste by Aspergillus carbonarius and Its Application in the Production of Inverted Sugar

Batista

Melo

Santos

et al. 2021

Food Technol. Biotechnol. (Online)

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Research background. Microbial β-fructofuranosidases are widely employed in food industry to produce inverted sugar or fructooligosaccharides. In this study, a newly isolated Aspergillus carbonarius PC-4 strain was used to optimize the β-fructofuranosidase production under a cost-effective process and the sucrose hydrolysis was evaluated to produce inverted sugars. Experimental approach. Optimization of nutritional components of culture medium was carried out using Simplex Lattice mixture design for 72 h and 120 h at 28 ˚C. One-Factor-at-a-time methodology was used to optimize the physicochemical parameters. Crude enzyme was used for sucrose hydrolysis at different concentrations. Results and conclusions. The optimized condition of enzyme production was pineapple crown waste (1.3 %, m/V) and yeast extract (0.3 %, m/V) for 72 h (9.4 U/mL), obtaining R² 91.85 %, R² adjusted 85.06 %, highest F value (13.52) and low p-value (0.003). One-factor-at-a-time used for optimizing the physicochemical conditions showed optimum temperature (20 ˚C), pH (5.5), agitation (180 rpm) and time-course (72 h) with an increase of 3.0-folds for enzyme production. The invertase-induced sucrose hydrolysis showed the maximum yield (3,451.7 µmols of reducing sugars) using 10 % of initial sucrose concentration. Higher sucrose concentrations caused inhibition of invertase activity, possibly due to saturation of substrate or formation of sucrose aggregates making it difficult for the enzyme to access sucrose molecules within the created clusters. So, a cost-effective method was developed for the invertase production using agroindustrial waste and the enzyme produced can be used efficiently for inverted sugar production at high sucrose concentration. Novelty and scientific contribution. This study presents an efficient utilization of pineapple crown wastes to produce invertase by a newly isolated Aspergillus carbonarius PC-4 strain. This enzyme exhibited a good potential for invert sugar production at high initial sucrose concentration, which is interesting for industrial applications.

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Overproduction of a β-fructofuranosidase1 with a high FOS synthesis activity for efficient biosynthesis of fructooligosaccharides

Cited by 31 publications

References 29 publications

Cunninghamella echinulata PA3S12MM invertase: Biochemical characterization of a promiscuous enzyme

Cunninghamella echinulata PA3S12MM invertase: Biochemical characterization of a promiscuous enzyme

Highly Efficient Production of Transfructosylating Enzymes Using Low-Cost Sugarcane Molasses by A. Pullulans FRR 5284

Optimization of β-Fructofuranosidase Production from Agrowaste by Aspergillus carbonarius and Its Application in the Production of Inverted Sugar

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