Overloading study of basic compounds with a positively charged C18 column in liquid chromatography

Wang, Chaoran; Guo, Zhimou; Zhang, Long; Zhang, Xiuli; Liang, Xinmiao

doi:10.1016/j.chroma.2013.01.074

Cited by 43 publications

(21 citation statements)

References 36 publications

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“…The peak broadening of the basic compound is very serious in Prep-LC and very sensitive to the amount of sample loading. Although the loadability may be improved at alkaline pH, due to the solubility of basic compounds at high pH mobile phases and high-pH-stable columns etc., it cannot completely solve the problems of bases [24][25][26].…”

Section: The Design and Selection Of The Samples And Columnsmentioning

confidence: 99%

Practical method for the definition of chromatographic peak parameters in preparative liquid chromatography

et al. 2016

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A practical method was established for the definition of chromatographic parameters in preparative liquid chromatography. The parameters contained both the peak broadening level under different amounts of sample loading and the concentration distribution of the target compound in the elution. The parameters of the peak broadening level were defined and expressed as a matrix, which consisted of sample loading, the forward broadening and the backward broadening levels. The concentration distribution of the target compound was described by the heat map of the elution profile. The most suitable stationary phase should exhibit the narrower peak broadening and it was best to broaden to both sides to compare to the peak under analytical conditions. Besides, the concentration distribution of the target compounds should be focused on the middle of the elution. The guiding principles were validated by purification of amitriptyline from the mixture of desipramine and amitriptyline. On the selected column, when the content of the impurity desipramine was lower than 0.1%, the recovery of target compound was much higher than the other columns even when the sample loading was as high as 8.03 mg/cm . The parameters and methods could be used for the evaluation and selection of stationary phases in preparative chromatography.

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Section: The Design and Selection Of The Samples And Columnsmentioning

confidence: 99%

Practical method for the definition of chromatographic peak parameters in preparative liquid chromatography

et al. 2016

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“…Waters ACQUITY BEH Shield RP18 column, a polar‐embedded column, was adopted in the quantitative determination of isoquinoline alkaloids from the roots of Hydrastis canadensis L. [7]. BEH Shield RP18 column was also proved to maintain satisfactory performance even with acidic additives of low ionic strength [55]. For instance, piperidine alkaloids from the herbs of Huperzia serrata (Thunb.…”

Section: Chromatographic Separation Methodsmentioning

confidence: 99%

“…While Acchrom Tech, a Chinese column supplier, launched a series of positive charge modified columns called “XCharge.” Both can greatly improve the loadability of alkaloids and acquire symmetrical peaks [57]. Moreover, it was reported that CSH analytical columns could provide excellent peak shape in the eluent system with weak acidity and low ionic strength, which is beneficial to the mass spectrometric analysis [55]. XCharge C 18 column was employed in the separation of polar and basic secondary metabolites including alkaloids from fruits of Macleaya cordata (Willd.)…”

Section: Chromatographic Separation Methodsmentioning

confidence: 99%

Liquid chromatographic separation of alkaloids in herbal medicines: Current status and perspectives

Zhao

Chen

Yu-qian

et al. 2020

J of Separation Science

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Alkaloids are a widespread group of basic compounds in herbal medicines and have attracted great interest due to various pharmaceutical activities and desirable druggability. Their distinctive structures make chromatographic separation fairly difficult. Peak tailing, poor resolution, and inferior column‐to‐column reproducibility are common obstacles to overcome. In order to provide a valuable reference, the methodologies and/or strategies on liquid chromatographic separation of alkaloids in herbal medicines proposed from 2012 to 2019 are thoroughly summarized.

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“…3c-j). The good performance for bases on this column could be explained using the multiple-site adsorption theory, in which the ionic repulsion would shield compounds from occupying high-energy sites in C18 deeper layer [80]. Using this method, 80 fractions of Corydalis yanhusuo W. T. were obtained, and then assayed for their ability to activate -opioid receptor.…”

Section: 22mentioning

confidence: 99%

Recent development in liquid chromatography stationary phases for separation of Traditional Chinese Medicine components

Jin

Liu

Guo

et al. 2016

Journal of Pharmaceutical and Biomedical Analysis

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Traditional Chinese Medicine (TCM) is an ancient medical practice which has been used to prevent and cure diseases for thousands of years. TCMs are frequently multi-component systems with mainly unidentified constituents. The study of the chemical compositions of TCMs remains a hotspot of research. Different strategies have been developed to manage the significant complexity of TCMs, in an attempt to determine their constituents. Reversed-phase liquid chromatography (RPLC) is still the method of choice for the separation of TCMs, but has many problems related to limited selectivity. Recently, enormous efforts have been concentrated on the development of efficient liquid chromatography (LC) methods for TCMs, based on selective stationary phases. This can improve the resolution and peak capacity considerably. In addition, high-efficiency stationary phases have been applied in the analysis of TCMs since the invention of ultra high-performance liquid chromatography (UHPLC). This review describes the advances in LC methods in TCM research from 2010 to date, and focuses on novel stationary phases. Their potential in the separation of TCMs using relevant applications is also demonstrated.

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Overloading study of basic compounds with a positively charged C18 column in liquid chromatography

Cited by 43 publications

References 36 publications

Practical method for the definition of chromatographic peak parameters in preparative liquid chromatography

Practical method for the definition of chromatographic peak parameters in preparative liquid chromatography

Liquid chromatographic separation of alkaloids in herbal medicines: Current status and perspectives

Recent development in liquid chromatography stationary phases for separation of Traditional Chinese Medicine components

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