Overlapping roles for PLK1 and aurora A during meiotic centrosome biogenesis in mouse spermatocytes

Wellard, Stephen R; Zhang, Yujiao; Shults, Chris; Zhao, Xueqi; McKay, Matthew J.; Murray, Stephen A.; Jordan, Philip W.

doi:10.15252/embr.202154106

Cited by 11 publications

(14 citation statements)

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“…On the one hand, we show that CEP164 serves to dock the basal body during zygotene ciliogenesis, sharing a similar role to their function in somatic cells [22,24,25]. On the other hand, spermatids present CEP164 distal appendages to promote the polymerization of the flagellar axoneme, as previously reported [44]. Hence, the dynamics of CEP164 seems to be essential for the progression of spermatogenesis.…”

Section: Meiotic Centrosomes and Their Relation To Ciliogenesis And F...supporting

confidence: 83%

“…We then tested if mouse meiotic cilia emanate from the mother centriole. For this purpose, we first studied the dynamics of CEP164, a protein of the distal appendages required for cilia formation in somatic cells [22,43] that has also been identified at the mother meiotic centriole in mouse spermatocytes [44]. Our results corroborated that CEP164 labeled one of the two centrioles before centrosome duplication at early prophase I.…”

Section: Mouse Meiotic Cilia Emanate From the Mother Centriole Prior ...supporting

confidence: 57%

“…At leptotene, CEP164 labels a circular signal around one of the two CETN3-labeled centrioles of the not yet duplicated centrosome (Figure 6 I. A and A’). Centrosome duplication occurs during zygotene [30, 44], and four signals of CETN3 were observed, but CEP164 still labeled only one of the four centrioles at this stage (Figure 6 I.B and B’). We then we triple immunolabelled CETN3, CEP164 and AcTub.…”

Section: Resultsmentioning

confidence: 99%

“…A and A´). Centrosome duplication occurs during zygotene [30,44], and four signals of CETN3 were observed, but CEP164 still labeled only one of the four centrioles at this stage (Figure 6 I.B and B´). We then we triple immunolabelled CETN3, CEP164 and AcTub.…”

Section: Mouse Meiotic Cilia Emanate From the Mother Centriole Prior ...mentioning

confidence: 97%

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The male mouse meiotic cilium emanates from the mother centriole at zygotene prior to centrosome duplication

López-Jiménez

Pérez-Martín

Hidalgo

et al. 2022

Preprint

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Cilia are hair-like projections of the plasma membrane with an inner microtubule skeleton known as axoneme. Motile cilia and flagella beat to displace extracellular fluids, playing important roles in the airways and reproductive system, among others. Instead, primary cilia function as cell type-dependent sensory organelles, detecting chemical, mechanical or optical signals from the extracellular environment. Cilia dysfunction is associated with genetic diseases called ciliopathies, and with some types of cancer.Cilia have been recently identified in zebrafish gametogenesis as an important regulator of thebouquetconformation and recombination. However, there is very little information about the structure and functions of cilia in mammalian meiosis. Here we describe the presence of cilia in male mouse meiotic cells. These solitary cilia form transiently in 20% of zygotene spermatocytes and reach considerable lengths (up to 15 μm). CEP164 and CETN3 localization studies indicate that these cilia emanate from the mother centriole, prior to centrosome duplication. In addition, the study of telomeric TFR2 suggests that these cilia are not directly related to thebouquetconformation during early male mouse meiosis. Instead, based on TEX14 labeling of intercellular bridges in spermatocyte cysts, we suggest that mouse meiotic cilia may have sensory roles affecting cyst function during prophase I.

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Section: Meiotic Centrosomes and Their Relation To Ciliogenesis And F...supporting

confidence: 83%

Section: Mouse Meiotic Cilia Emanate From the Mother Centriole Prior ...supporting

confidence: 57%

Section: Resultsmentioning

confidence: 99%

Section: Mouse Meiotic Cilia Emanate From the Mother Centriole Prior ...mentioning

confidence: 97%

See 2 more Smart Citations

The male mouse meiotic cilium emanates from the mother centriole at zygotene prior to centrosome duplication

López-Jiménez

Pérez-Martín

Hidalgo

et al. 2022

Preprint

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show abstract

“…In addition, PLK1 is required for centrosome maturation and separation prior to metaphase I, leading to the formation of bipolar metaphase I spindles. PLK1 is vital for proficient centrosome biogenesis and accurate chromosome segregation during spermatogenesis (43). Herein, pathogenic mutations of PLK1 (c.1210G>T; p.A404S) were detected in the husband of a patient who had two miscarriages.…”

Section: Discussionmentioning

confidence: 95%

Identification of genetic polymorphisms in unexplained recurrent spontaneous abortion based on whole exome sequencing

Mou

Huang

et al. 2022

Ann Transl Med

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Background: The precise etiology of approximately 50% of patients with recurrent spontaneous abortion (RSA) is unclear, known as unexplained recurrent spontaneous abortion (URSA). This study identified the genetic polymorphisms in patients with URSA.Methods: Genomic DNA was extracted from 30 couples with URSA and 9 couples with normal reproductive history for whole exome sequencing. Variations in annotation, filtering, and prediction of harmfulness and pathogenicity were examined. Furthermore, predictions of the effects of changes in protein structure, Sanger validation, and functional enrichment analyses were performed. The missense mutated genes with significant changes in protein function, and genes with mutations of premature stop, splice site, frameshift, and in-frame indel were selected as candidate mutated genes related to URSA.Results: In 30 unrelated couples with URSA, 50%, 20%, and 30% had 2, 3, and more than 4 miscarriages, respectively. Totally, 971 maternal and 954 paternal mutations were found to be pathogenic or possibly pathogenic after preliminary filtering. Total variations were not associated with age nor the number of miscarriages. In 28 patients (involving 23 couples), 22 pathogenic or possibly pathogenic variants of 19 genes were found to be strongly associated with URSA, with an abnormality rate of 76.67%. Among these, 12 missense variants showed obvious changes in protein functions, including ANXA5 (c.949G>C; p.G317R),

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Arf1 GTPase Regulates Golgi‐Dependent G2/M Transition and Spindle Organization in Oocyte Meiosis

Zhang,

Zou,

Shan

et al. 2023

Advanced Science

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ADP‐ribosylation factor 1 (Arf1) is a small GTPase belonging to the Arf family. As a molecular switch, Arf1 is found to regulate retrograde and intra‐Golgi transport, plasma membrane signaling, and organelle function during mitosis. This study aimed to explore the noncanonical roles of Arf1 in cell cycle regulation and cytoskeleton dynamics in meiosis with a mouse oocyte model. Arf1 accumulated in microtubules during oocyte meiosis, and the depletion of Arf1 led to the failure of polar body extrusion. Unlike mitosis, it finds that Arf1 affected Myt1 activity for cyclin B1/CDK1‐based G2/M transition, which disturbed oocyte meiotic resumption. Besides, Arf1 modulated GM130 for the dynamic changes in the Golgi apparatus and Rab35‐based vesicle transport during meiosis. Moreover, Arf1 is associated with Ran GTPase for TPX2 expression, further regulating the Aurora A–polo‐like kinase 1 pathway for meiotic spindle assembly and microtubule stability in oocytes. Further, exogenous Arf1 mRNA supplementation can significantly rescue these defects. In conclusion, results reported the noncanonical functions of Arf1 in G2/M transition and meiotic spindle organization in mouse oocytes.

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Overlapping roles for PLK1 and aurora A during meiotic centrosome biogenesis in mouse spermatocytes

Cited by 11 publications

References 1 publication

The male mouse meiotic cilium emanates from the mother centriole at zygotene prior to centrosome duplication

The male mouse meiotic cilium emanates from the mother centriole at zygotene prior to centrosome duplication

Identification of genetic polymorphisms in unexplained recurrent spontaneous abortion based on whole exome sequencing

Arf1 GTPase Regulates Golgi‐Dependent G2/M Transition and Spindle Organization in Oocyte Meiosis

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