Overlapping functions of recD, recJ and recN provide evidence of three epistatic groups of genes in Escherichia coli recombination and DNA repair

Lloyd, Robert G.; Buckman, Carol

doi:10.1016/0300-9084(91)90218-p

Cited by 64 publications

(52 citation statements)

References 39 publications

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“…Sci. USA 93 (1996) 14471 recombination and repair (7,8,11,18). It, however, presents a problem, which is the function of RecF protein in this complex.…”

Section: Discussionmentioning

confidence: 99%

“…Several genetic studies have suggested that the products of the recO and recR genes are involved and presumably interact with RecF in many of these processes. (i) The recF, recO, and recR genes belong to the same epistasis group (7). (ii) Certain phenotypes associated with recF, recO, and recR are suppressed by recA803 (8)(9)(10), recA441, recA730, and other recA mutations (11).…”

mentioning

confidence: 99%

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Interactions of RecF protein with RecO, RecR, and single-stranded DNA binding proteins reveal roles for the RecF–RecO–RecR complex in DNA repair and recombination

Hegde

Qin

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

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“…Sci. USA 93 (1996) 14471 recombination and repair (7,8,11,18). It, however, presents a problem, which is the function of RecF protein in this complex.…”

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Interactions of RecF protein with RecO, RecR, and single-stranded DNA binding proteins reveal roles for the RecF–RecO–RecR complex in DNA repair and recombination

Hegde

Qin

et al. 1996

Proc. Natl. Acad. Sci. U.S.A.

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“…Group -y includes the recP and recH mutations. Similarly, Lloyd and Buckman (22) examined DNA repair and recombination in E. coli strains carrying various mutation combinations in two or more RecF pathway genes and reported that the recombination genes in E. coli also define three epistatic groups.…”

Section: Methodsmentioning

confidence: 99%

Characterization of Bacillus subtilis recombinational pathways

1991

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Recombination in Bacillus subtilis requires the products of numerous rec loci. To dissect the various mechanisms which may be involved in genetic recombination, we constructed a series of isogenic strains containing more than one mutant rec allele. On the basis of their impairment in genetic exchange, the various loci (represented by specific rec alleles) were classified into different epistatic groups. Group alpha consists of rec genes represented by recB, recD, recF, recG, recL, and recR mutations, while group beta comprises the addA and addB mutations. Group gamma consists of the recH and recP mutations. These results suggest that B. subtilis has multiple pathways for genetic recombination and that the products of the genes within the alpha, beta, and gamma epistatic groups are involved in these alternative recombination pathways. The RecA protein is required in all three pathways of intermolecular recombination.

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“…The RecF recombination pathway was originally characterized in strains that are defective for RecBCD, SbcB and SbcCD (reviewed in Clark and Sandler, 1994). In these strains, RecF may act in combination with different presynaptic and post-synaptic enzymes (Lloyd and Buckman, 1991;Clark and Sandler, 1994;. Interestingly, the RecF recombination pathway activated by the uvrD mutation differs from the previously described ones, as (i) it is active in the presence of RecBCD, SbcB and SbcCD proteins; (ii) it requires SOS induction and UvrD inactivation; and (iii) it depends on a specific presynaptic protein, RecQ, and a specific post-synaptic complex, RuvAB.…”

Section: Recf-dependent Pathway For Uvrd-stimulated Recombinationmentioning

confidence: 99%

uvrD mutations enhance tandem repeat deletion in the Escherichia coli chromosome via SOS induction of the RecF recombination pathway

Bierne

Seigneur

et al. 1997

Molecular Microbiology

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SummaryIt has previously been shown that recombination between tandem repeats is not significantly affected by a recA mutation in Escherichia coli. Here, we describe the activation of a RecA-dependent recombination pathway in a hyper-recombination mutant. In order to analyse how tandem repeat deletion may proceed, we searched for mutants that affect this process. Three hyper-recombination clones were characterized and shown to be mutated in the uvrD gene. Two of the mutations were identified as opal mutations at codons 130 and 438. A uvrD ::Tn5 mutation was used to investigate the mechanism of deletion formation in these mutants. The uvrD-mediated stimulation of deletion was abolished by a lexAind3 mutation or by inactivation of either the recA, recF, recQ or ruvA genes. We conclude that (i) this stimulation requires SOS induction and (ii) tandem repeat recombination in uvrD mutants occurs via the RecF pathway. In uvrD þ cells, constitutive expression of SOS genes is not sufficient to stimulate deletion formation. This suggests that the RecF recombination pathway activated by SOS induction is antagonized by the UvrD protein. Paradoxically, we observed that the overproduction of UvrD from a plasmid also stimulates tandem repeat deletion. However, this stimulation is RecA independent, as is deletion in a wild-type strain. We propose that the presence of an excess of the UvrD helicase favours replication slippage. This work suggests that the UvrD helicase controls a balance between different routes of tandem repeat deletion.

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Overlapping functions of recD, recJ and recN provide evidence of three epistatic groups of genes in Escherichia coli recombination and DNA repair

Cited by 64 publications

References 39 publications

Interactions of RecF protein with RecO, RecR, and single-stranded DNA binding proteins reveal roles for the RecF–RecO–RecR complex in DNA repair and recombination

Interactions of RecF protein with RecO, RecR, and single-stranded DNA binding proteins reveal roles for the RecF–RecO–RecR complex in DNA repair and recombination

Characterization of Bacillus subtilis recombinational pathways

uvrD mutations enhance tandem repeat deletion in the Escherichia coli chromosome via SOS induction of the RecF recombination pathway

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