Overlapping expression of microRNAs in human embryonic colon and colorectal cancer

Monzó, Mariano; Navarro, Alfons; Bandrés, Eva; Artells, Rosa; Moreno, Isabel; Gel, Bernat; Ibeas, R.; Moreno, José; Martínez, Francisco García; Díaz, Tania; Martı́nez, Antonio; Balagué, Olga; Garcı́a-Foncillas, Jesús

doi:10.1038/cr.2008.81

Cited by 165 publications

(133 citation statements)

References 29 publications

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“…We identified 19 new miRNAs deregulated in CRC ( Table 1) that, to our knowledge, have never before been reported in cancer. We found deregulation of miR-1, miR-195, miR-143, miR-215, miR-31, miR-194, miR-30c, miR-133b, miR-192, miR-30b, miR-378*, miR-137, miR-133a, and miR-378 in CRC, which is consistent with earlier reports of miRNA expression in CRC (Michael et al, 2003;Bandres et al, 2006;Cummins et al, 2006;Volinia et al, 2006;Monzo et al, 2008;Arndt et al, 2009;Earle et al, 2010;Wang et al, 2010). Down-regulation of miR-378 in CRC compared with normal, observed in both our microarray and RT-PCR analysis, was also seen in a study by Wang et al (2010), who compared para-cancerous tissues and colorectal cancer samples.…”

Section: Mirna Profiling In Crc Versus Normal Colon Tissuesupporting

confidence: 92%

“…A number of studies published recently focus on the significance of miRNAs in CRC development, classification, diagnosis, and prognosis (Michael et al, 2003;Bandres et al, 2006;Cummins et al, 2006;Volinia et al, 2006;Monzo et al, 2008;Earle et al, 2010). Little is known, however, about differences in miRNA expression between CRCs with and without the KRAS mutation.…”

Section: Introductionmentioning

confidence: 99%

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MicroRNA profiling differentiates colorectal cancer according to KRAS status

Mosakhani

Sarhadi

Borze

et al. 2011

Genes Chromosomes & Cancer

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Recent studies have shown the important role of microRNAs (miRNAs) in a variety of biological processes, and in its ability to distinguish tumors according to their prognostic and predictive properties. To identify miRNA signatures associated with colorectal carcinoma (CRC) and with KRAS status, we studied, using Agilent's miRNA microarrays, miRNA expression in primary tumors from 55 metastatic CRC patients, including 15 with mutant and 40 with wild-type KRAS. Comparing these with normal colon tissue, we identified 49 miRNAs-including 19 novel miRNAs-significantly deregulated in tumor tissue. The presence of the KRAS mutation was associated with up-regulation of miR-127-3p, miR-92a, and miR-486-3p and down-regulation of miR-378. Increased expression of miR-127-3p and miR-92a in KRAS mutant tumors was significantly confirmed by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) (P < 0.05). We identified some predicted target genes of differentially expressed miRNAs between mutated and wild-type KRAS, such as RSG3 and TOB1, which are involved in apoptosis and proliferation. Target prediction and pathway analysis suggest a possible role for deregulated miRNAs in nicotinamide adenine dinucleotide phosphate (NADPH) regeneration and G protein-coupled receptor signaling pathways.

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Section: Mirna Profiling In Crc Versus Normal Colon Tissuesupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

MicroRNA profiling differentiates colorectal cancer according to KRAS status

Mosakhani

Sarhadi

Borze

et al. 2011

Genes Chromosomes & Cancer

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“…The function of miRNA is the downregulation of multiple target gene expression by degrading the mRNA or blocking its translation into protein through RNA interference (24,25). Several miRNAs, such as miRNA-21 (miR-21), miR-17-92 cluster, and miR-135, were highly expressed in CRC tissues (26)(27)(28)(29). Several recent studies reported that the circulating miRNA in plasma was a potential marker for CRC detection (30,31) and was remarkably stable in plasma and protected from endogenous RNase activity (32).…”

Section: Introductionmentioning

confidence: 99%

MicroRNA Expression Profiling of Exfoliated Colonocytes Isolated from Feces for Colorectal Cancer Screening

Koga

Yasunaga

Takahashi

et al. 2010

Cancer Prevention Research

189

167

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To reduce the colorectal cancer (CRC) mortality rate, we have reported several CRC screening methods using colonocytes isolated from feces. Expression analysis of oncogenic microRNA (miRNA) in peripheral blood was recently reported for CRC detection. In the present study, we conducted miRNA expression analysis of exfoliated colonocytes isolated from feces for CRC screening. Two hundred six CRC patients and 134 healthy volunteers were enrolled in the study. miRNA expression of the miR-17-92 cluster, miR-21, and miR-135 in colonocytes isolated from feces as well as frozen tissues was analyzed by quantitative realtime PCR. The expression of the miR-17-92 cluster, miR-21, and miR-135 was significantly higher in CRC tissues compared with normal tissues. The exfoliated colonocytes of 197 CRC patients and 119 healthy volunteers were analyzed because of the presence of sufficient miRNA concentration. miR-21 expression did not differ significantly between CRC patients and healthy volunteers (P = 0.6). The expression of miR-17-92 cluster and miR-135 was significantly higher in CRC patients than in healthy volunteers (P < 0.0001). The overall sensitivity and specificity by using miRNA expression was 74.1% (146/197; 95% confidence interval, 67.4-80.1) and 79.0% (94/119; 95% confidence interval, 70.6-85.9), respectively. Sensitivity was dependent only on tumor location (P = 0.0001). miRNA was relatively well conserved in exfoliated colonocytes from feces both of CRC patients and healthy volunteers. miRNA expression analysis of the isolated colonocytes may be a useful method for CRC screening. Furthermore, oncogenic miRNA highly expressed in CRC should be investigated for CRC screening tests in the future. Cancer Prev Res; 3(11); 1435-42. ©2010 AACR.

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“…Overlapping miRNA expression was detected between embryonic colonic mucosa and CRC. The miR-17-92 cluster and its target, E2F1, exhibit a similar pattern of expression in human colon development and in colonic carcinogenesis -regulating cell proliferation in both cases [59]. Authors of this study conclude that miRNA pathways play a major role in both embryonic development and neoplastic transformation of the colonic epithelium.…”

Section: Mir-96 Mir-135b Mir-183 (Up-regulated In Tumors and Crc Cementioning

confidence: 53%

“…Monzo et al [59] assessed the expression of mature miRNAs in human embryonic colon tissue, as well as in CRC and paired normal colon tissue. Overlapping miRNA expression was detected between embryonic colonic mucosa and CRC.…”

Section: Mir-96 Mir-135b Mir-183 (Up-regulated In Tumors and Crc Cementioning

confidence: 99%

MicroRNAs in Colorectal Cancer: Translation of Molecular Biology into Clinical Application

Svoboda¹

2010

J Mol Biomark Diagn

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MicroRNAs (miRNAs) are small non-coding RNAs 18-25 nucleotides in length that downregulate gene expression during various crucial cell processes such as apoptosis, differentiation and development. Changes in the expression profiles of miRNAs have been observed in a variety of human tumors, including colorectal cancer (CRC). Functional studies indicate that miRNAs act as tumor suppressors and oncogenes. These findings significantly extend Vogelstein's model of CRC pathogenesis and have shown great potential for miRNAs as a novel class of therapeutic targets. Several investigations have also described the ability of miRNA expression profiles to predict prognosis and response to selected treatments in CRC patients, and support diagnosis of CRC among cancer of unknown primary site. miRNAs' occurrence has been repeatedly observed also in serum and plasma, and miRNAs as novel minimally invasive biomarkers have indicated reasonable sensitivity for CRC detection and compare favorably with the fecal occult blood test. In this review, we summarize the knowledge regarding miRNAs' functioning in CRC while emphasizing their significance in pathogenetic signaling pathways and their potential to serve as disease biomarkers and novel therapeutic targets.

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Overlapping expression of microRNAs in human embryonic colon and colorectal cancer

Cited by 165 publications

References 29 publications

MicroRNA profiling differentiates colorectal cancer according to KRAS status

MicroRNA profiling differentiates colorectal cancer according to KRAS status

MicroRNA Expression Profiling of Exfoliated Colonocytes Isolated from Feces for Colorectal Cancer Screening

MicroRNAs in Colorectal Cancer: Translation of Molecular Biology into Clinical Application

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