Overlapping but Distinct RNA Elements Control Repression and Activation of nanos Translation

Crucs, Susan; Chatterjee, Seema; Gavis, Elizabeth R.

doi:10.1016/s1097-2765(00)80440-2

Cited by 95 publications

(91 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The structural context of the motif was also important for She-complex recognition: Positioning the motif in a duplex abolished activity, and increasing the distance between the motif and adjacent stem decreased activity in three of four zipcodes. A simple sequence motif stabilized by surrounding secondary structure appears to be a common theme of many protein binding sites in mRNAs, e.g., the Smg binding site in nos RNA (25). The She2͞3 recognition site defined in this work expands on the CGA triplet reported by Olivier et al (12) by virtue of a larger set of zipcodes that allowed us to identify the more degenerate bases downstream of the triplet as part of the recognition site.…”

Section: Complex Sequence and Structural Features Mediate She2͞3 Bindmentioning

confidence: 78%

Unbiased selection of localization elements reveals cis-acting determinants of mRNA bud localization in Saccharomyces cerevisiae

Jambhekar

McDermott

Sorber

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Cytoplasmic mRNA localization is a mechanism used by many organisms to generate asymmetry and sequester protein activity. In the yeast Saccharomyces cerevisiae, mRNA transport to bud tips of dividing cells is mediated by the binding of She2p, She3p, and Myo4p to coding regions of the RNA. To date, 24 bud-localized mRNAs have been identified, yet the RNA determinants that mediate localization remain poorly understood. Here, we used nonhomologous random recombination to generate libraries of sequences that could be selected for their ability to bind Shecomplex proteins, thereby providing an unbiased approach for minimizing and mapping localization elements in several transported RNAs. Analysis of the derived sequences and predicted secondary structures revealed short sequence motifs that mediate binding to the She complex and RNA localization to the bud tip in vivo. A predicted single-stranded core CG dinucleotide appears to be an important component of the RNA-protein interface, although other nucleotides contribute in a context-dependent manner. Our findings further our understanding of RNA recognition by the She complex, and the methods used here should be applicable for elucidating minimal RNA motifs involved in many other types of interactions.three-hybrid selection ͉ nonhomologous random recombination ͉ RNA zipcode

show abstract

Section: Complex Sequence and Structural Features Mediate She2͞3 Bindmentioning

confidence: 78%

Unbiased selection of localization elements reveals cis-acting determinants of mRNA bud localization in Saccharomyces cerevisiae

Jambhekar

McDermott

Sorber

et al. 2005

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Selectivity arises when their target RNA sequences are compared. As opposed to the NRE that Pum2 binds, Smaug binds the Smaug recognition element (Crucs et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Dendritic Localization of the Translational Repressor Pumilio 2 and Its Contribution to Dendritic Stress Granules

Vessey

Vaccani

Xie

et al. 2006

Journal of Neuroscience

162

178

View full text Add to dashboard Cite

Pumilio (Pum) protein acts as a translational inhibitor in several organisms including yeast, Drosophila, Xenopus, and mammals. Two Pumilio genes, Pum1 and Pum2, have been identified in mammals, but their function in neurons has not been identified. In this study, we found that Pum2 mRNA is expressed during neuronal development and that the protein is found in discrete particles in both the cell body and the dendritic compartment of fully polarized neurons. This finding indicates that Pum2 is a novel candidate of dendritically localized ribonucleoparticles (RNPs). During metabolic stress, Pum2 is present in stress granules (

show abstract

“…Translation control elements (TCE) are short elements (∼ 90 nt) found in the 3'UTR of nanos mRNA of drosophila [69]. Its secondary structure is composed of a helix and a multiloop with two hairpin loops branching off, one with a conserved primary structure in the hairpin.…”

Section: Important Regulatory Motifs In Utrs Of Mrnasmentioning

confidence: 99%

Evolutionary patterns of non-coding RNAs

Bompfünewerer

Flamm

Fried

et al. 2005

Theory in Biosciences

View full text Add to dashboard Cite

A plethora of new functions of non-coding RNAs have been discovered in past few years. In fact, RNA is emerging as the central player in cellular regulation, taking on active roles in multiple regulatory layers from transcription, RNA maturation, and Manuscript January 2005RNA modification to translational regulation. Nevertheless, very little is known about the evolution of this "Modern RNA World" and its components. In this contribution we attempt to provide at least a cursory overview of the diversity of non-coding RNAs and functional RNA motifs in non-translated regions of regular messenger RNAs (mRNAs) with an emphasis on evolutionary questions. This survey is complemented by an in-depth analysis of examples from different classes of RNAs focusing mostly on their evolution in the vertebrate lineage. We present a survey of Y RNA genes in vertebrates, studies of the molecular evolution of the U7 snRNA, the snoRNAs E1/U17, E2, and E3, the Y RNA family, the let-7 microRNA family, and the mRNA-like evf-1 gene. We furthermore discuss the statistical distribution of microRNAs in metazoans, which suggests an explosive increase in the microRNA repertoire in vertebrates. The analysis of the transcription of non-coding RNAs (ncRNAs) suggests that small RNAs in general are genetically mobile in the sense that their association with a hostgene (e.g. when transcribed from introns of a mRNA) can change on evolutionary time scales. The let-7 family demonstrates, that even the mode of transcription (as intron or as exon) can change among paralogous ncRNA.

show abstract

Overlapping but Distinct RNA Elements Control Repression and Activation of nanos Translation

Cited by 95 publications

References 37 publications

Unbiased selection of localization elements reveals cis-acting determinants of mRNA bud localization in Saccharomyces cerevisiae

Unbiased selection of localization elements reveals cis-acting determinants of mRNA bud localization in Saccharomyces cerevisiae

Dendritic Localization of the Translational Repressor Pumilio 2 and Its Contribution to Dendritic Stress Granules

Evolutionary patterns of non-coding RNAs

Contact Info

Product

Resources

About