Overexpression, purification, and biochemical characterization of GumC, an enzyme involved in the biosynthesis of exopolysaccharide by Xylella fastidiosa

Pieri, Celina de; Beltramini, Leila Maria; Selistre-de-Araújo, Heloísa Sobreiro; Vettore, André Luiz; Silva, Felipe Rodrigues da; Arruda, Paulo; Oliva, Glaucius; Souza, Dulce Helena Ferreira de

doi:10.1016/j.pep.2003.11.003

Cited by 5 publications

(2 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…X. fastidiosa survival inside xylem of plant and insect vectors (9). GumC may be responsible for regulating gum polymerization or secretion of fastidian gum (12). There was an increased expression level of gumC in DAC1984, indicating that gumC is repressed by GacA in vitro.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of Regulatory Pathways in Xylella fastidiosa : Genes and Phenotypes Controlled by gacA

Shi

Dumenyo

Hernández-Martínez

et al. 2009

Appl Environ Microbiol

View full text Add to dashboard Cite

The xylem-limited, insect-transmitted bacterium Xylella fastidiosa causes Pierce's disease in grapes through cell aggregation and vascular clogging. GacA controls various physiological processes and pathogenicity factors in many gram-negative bacteria, including biofilm formation in Pseudomonas syringae pv. tomato DC3000. Cloned gacA of X. fastidiosa was found to restore the hypersensitive response and pathogenicity in gacA mutants of P. syringae pv. tomato DC3000 and Erwinia amylovora. A gacA mutant of X. fastidiosa (DAC1984) had significantly reduced abilities to adhere to a glass surface, form biofilm, and incite disease symptoms on grapevines, compared with the parent (A05). cDNA microarray analysis identified 7 genes that were positively regulated by GacA, including xadA and hsf, predicted to encode outer membrane adhesion proteins, and 20 negatively regulated genes, including gumC and an antibacterial polypeptide toxin gene, cvaC. These results suggest that GacA of X. fastidiosa regulates many factors, which contribute to attachment and biofilm formation, as well as some physiological processes that may enhance the adaptation and tolerance of X. fastidiosa to environmental stresses and the competition within the host xylem.

show abstract

Section: Discussionmentioning

confidence: 99%

“…PD progression in grapevines inoculated with wild-type X. fastidiosa A05 and gacA mutant DAC1984. Disease severity was based on a visual disease scale of 0 to 5 and was assessed 4,8,12,16, and 20 weeks after inoculation (16). The data are averages for 10 independent replications.…”

Section: Discussionmentioning

confidence: 99%