Overexpression of β‐expansin gene <i><scp>GmEXPB2</scp></i> improves phosphorus efficiency in soybean

Zhou, Jia; Xie, Jiawei; Liao, Hong; Wang, Xiurong

doi:10.1111/ppl.12077

Cited by 63 publications

(49 citation statements)

References 51 publications

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“…Four types of cis-acting elements, light responsive elements, hormone responsive elements, environmental stress-related elements, and development related elements, were found to be apparently abundant for the NtEXPs. In plants, the expression of expansins are regulated by both internal and external factors, resulting in their involvement in a variety of developmental processes, such as seed germination , fruit growth and/or ripening (Rose et al 1997;Brummell et al 1999;Ishimaru et al 2007), as well as in nutrient uptake and efficiency (Zhou et al 2014), and stress tolerance Zhao et al 2011;Lü et al 2013), which are conjured to be regulated by the corresponding cis-acting elements they contain. Plant hormones can regulate the expression of expansin genes and the regulation of expansin activity by plant hormones is well documented (Zhao et al 2012;Li et al 2014;Lu et al 2016).…”

Section: Discussionmentioning

confidence: 99%

“…The regulation of plant architecture by expansins to adapt to certain external environmental changes, such as drought, heat, low phosphorus (P), is also well studied (Cosgrove 2015;Lu et al 2016). Some examples include the wheat (Triticum aestivum) TaEXPB23, Poa pratens PpEXPA1, soybean GmEXPB2 and rose (Rosa hybrida) RhEXPA4, the transgenic plants with which gained tolerance to drought (Li et al , 2013, tolerance to heat (Xu et al 2014), improved P efficiency Zhou et al 2014) and tolerance to drought and salt (Lü et al 2013;Yan et al 2014), respectively. In addition, we found that for many cis-elements two or more copies located at adjacent sites, which might enhance the effects of their binding to trans-acting factors such as transcription factors.…”

Section: Discussionmentioning

confidence: 99%

“…Expansins are commonly found in plants and are well known to play important roles in diverse biological processes related to cell wall modification, including seed germination , root growth and development Yu et al 2011), stem growth and internode elongation (Cho and Kende 1997;Kuluev et al 2014), leaf initiation and expansion (Fleming et al 1997;Kuluev et al 2013), flowering and flower size (Kuluev et al 2012), pollen germination and fertilization (Cosgrove 2000a), fruit growth and/or ripening (Rose et al 1997;Brummell et al 1999;Civello et al 1999;Ishimaru et al 2007). They have also been associated with nutrient uptake and efficiency (Zhou et al 2014), abiotic and biotic stress tolerance Zhao et al 2011;Lü et al 2013). After looking closely at the various roles played by expansins in plants, Marowa et al (2016) have concluded that inclusion of expansins in plant breeding programs presents an opportunity to improve crops in various aspects.…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide identification of the expansin gene family in tobacco (Nicotiana tabacum)

2016

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Expansins are pH-dependent cell wall loosening proteins which form a large family in plants. They have been shown to be involved in various developmental processes and been implicated in enabling plants' ability to absorb nutrients from the soil as well as conferring biotic and abiotic stress resistances. It is therefore clear that they can be potential targets in genetic engineering for crop improvement. Tobacco (Nicotiana tabacum) is a major crop species as well as a model organism. Considering that only a few tobacco expansins have been studied, a genome-wide analysis of the tobacco expansin gene family is necessary. In this study, we identified 52 expansins in tobacco, which were classified into four subfamilies: 36 NtEXPAs, 6 NtEXPBs, 3 NtEXLAs and 7 NtEXLBs. Compared to other species, the NtEXLB subfamily size was relatively larger. Phylogenetic analysis showed that the 52 tobacco expansins were divided into 13 subgroups. Gene structure analysis revealed that genes within subfamilies/subgroups exhibited similar characteristics such as gene structure and protein motif arrangement. Whole-genome duplication and tandem duplication events may have played important roles in the expanding of tobacco expansins. Cis-Acting element analysis revealed that each expansin gene was regulated or several expansin genes were co-regulated by both internal and environmental factors. 35 of these genes were identified as being expressed according to a microarray analysis. In contrast to most NtEXPAs which had higher expression levels in young organs, NtEXLAs and NtEXLBs were preferentially expressed in mature or senescent tissues, suggesting that they might play different roles in different organs or at different developmental stages. As the first step towards genome-wide analysis of the tobacco expansin gene family, our work provides solid background information related to structure, evolution and expression as well as regulatory cis-acting elements of the tobacco expansins. This information will provide a strong foundation for cloning and functional exploration of expansin genes in tobacco.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genome-wide identification of the expansin gene family in tobacco (Nicotiana tabacum)

2016

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“…To explore effects of GmEXPB2 on nodule organogenesis, soybean transgenic composites and whole transgenic plants were generated according to Guo et al (2011) and Zhou et al (2014), respectively. The transgenic composite plants included GmEXPB2 OX, suppression (RNAi), and empty vector (CK) lines.…”

Section: Characterization Of Nodule Organogenesis Using Soybean Wholementioning

confidence: 99%

GmEXPB2, a Cell Wall β-Expansin Gene, Affects Soybean Nodulation through Modifying Root Architecture and Promoting Nodule Formation and Development

Zhao

Tan

et al. 2015

Plant Physiol.

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Nodulation is an essential process for biological nitrogen (N 2 ) fixation in legumes, but its regulation remains poorly understood. Here, a b-expansin gene, GmEXPB2, was found to be critical for soybean (Glycine max) nodulation. GmEXPB2 was preferentially expressed at the early stage of nodule development. b-Glucuronidase staining further showed that GmEXPB2 was mainly localized to the nodule vascular trace and nodule vascular bundles, as well as nodule cortical and parenchyma cells, suggesting that GmEXPB2 might be involved in cell wall modification and extension during nodule formation and development. Overexpression of GmEXPB2 dramatically modified soybean root architecture, increasing the size and number of cortical cells in the root meristematic and elongation zones and expanding root hair density and size of the root hair zone. Confocal microscopy with green fluorescent protein-labeled rhizobium USDA110 cells showed that the infection events were significantly enhanced in the GmEXPB2-overexpressing lines. Moreover, nodule primordium development was earlier in overexpressing lines compared with wild-type plants. Thereby, overexpression of GmEXPB2 in either transgenic soybean hairy roots or whole plants resulted in increased nodule number, nodule mass, and nitrogenase activity and thus elevated plant N and phosphorus content as well as biomass. In contrast, suppression of GmEXPB2 in soybean transgenic composite plants led to smaller infected cells and thus reduced number of big nodules, nodule mass, and nitrogenase activity, thereby inhibiting soybean growth. Taken together, we conclude that GmEXPB2 critically affects soybean nodulation through modifying root architecture and promoting nodule formation and development and subsequently impacts biological N 2 fixation and growth of soybean.

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“…These are changes that are observed in response to drought condition , Zhou et al, 2014. In 2003, Lee et al demonstrated the role of GmEXP1 in cell wall expansion at the developing site of the tobacco plant root.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of a soybean expansin gene, GmEXP1, improvesdrought tolerance in transgenic tobacco

Lo¹,

Le²,

Nguyen³

et al. 2015

Turk J Bot

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The EXP1 gene encodes expansin, which has the ability to loosen the plant cell wall. The soybean expansin gene GmEXP1 is activated specifically during the root elongation process, and thus it plays important roles in root development. During the drought period, changes in pressure within the cell and the fast development of the root allow plants to collect water from deep soil, which in turn helps plants grow and develop. In this study, we have successfully cloned and generated a GmEXP1 construct expressing recombinant expansin protein in tobacco plants. GmEXP1 is expressed in transgenic tobacco plants and passed on to the next generation. The transgenic tobacco plants have improved drought tolerance, which is demonstrated in both the length and volume of roots. From these promising results, we applied the same approach to generate drought-tolerant plants.

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Overexpression of β‐expansin gene GmEXPB2 improves phosphorus efficiency in soybean

Cited by 63 publications

References 51 publications

Genome-wide identification of the expansin gene family in tobacco (Nicotiana tabacum)

Genome-wide identification of the expansin gene family in tobacco (Nicotiana tabacum)

GmEXPB2, a Cell Wall β-Expansin Gene, Affects Soybean Nodulation through Modifying Root Architecture and Promoting Nodule Formation and Development

Overexpression of a soybean expansin gene, GmEXP1, improvesdrought tolerance in transgenic tobacco

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