Overexpression of XMyoD or XMyf5 in Xenopus Embryos Induces the Formation of Enlarged Myotomes through Recruitment of Cells of Nonsomitic Lineage

Ludolph, David C.; Neff, Anton W.; Mescher, Anthony L.; Malacinski, George M.; Parker, Marcie; Smith, Rosamund C.

doi:10.1006/dbio.1994.1294

Cited by 32 publications

(28 citation statements)

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“…The MyoD-injected side of the embryo displayed a dorsoanterior bulge, comprising an enlarged myotome and, in addition, a large area between the differentiated myotome and the epidermis that does not stain for muscle or neural markers (Fig. 3F, data not shown) (Ludolph et al, 1994). Strikingly, there were more ph3-positive cells on the injected side than the uninjected side of the embryo, indicating that MyoD overexpression can promote proliferation under some circumstances (100% of embryos, n=9) (Fig.…”

Section: Myod Does Not Upregulate P27 Xic1mentioning

confidence: 95%

A single cdk inhibitor, p27Xic1, functions beyond cell cycle regulation to promote muscle differentiation inXenopus

Vernon

Philpott

2003

Development

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The molecular basis of the antagonism between cellular proliferation and differentiation is poorly understood. We have investigated the role of the cyclin-dependent kinase inhibitor p27 Xic1 in the co-ordination of cell cycle exit and differentiation during early myogenesis in vivo using Xenopus embryos. In this report, we demonstrate that p27 Xic1 is highly expressed in the developing myotome, that ablation of p27 Xic1 protein prevents muscle differentiation and that p27 Xic1 synergizes with the transcription factor MyoD to promote muscle differentiation. Furthermore, the ability of p27 Xic1 to promote myogenesis resides in an Nterminal domain and is separable from its cell cycle regulation function. This data demonstrates that a single cyclin-dependent kinase inhibitor, p27 Xic1 , controls in vivo muscle differentiation in Xenopus and that regulation of this process by p27 Xic1 requires activities beyond cell cycle inhibition.

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Section: Myod Does Not Upregulate P27 Xic1mentioning

confidence: 95%

A single cdk inhibitor, p27Xic1, functions beyond cell cycle regulation to promote muscle differentiation inXenopus

Vernon

Philpott

2003

Development

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“…In Xenopus, in vivo analysis indicated that transcripts for each XMRF can induce a myogenic program after ectopic microinjection in 2-to 32-cell embryos (Ludolph et al, 1994; Fig. 3).…”

Section: In Vivo Xenopus Datamentioning

confidence: 98%

Myogenic regulatory factors: Redundant or specific functions? Lessons from Xenopus

Chanoine

Gaspera

Charbonnier

2004

Developmental Dynamics

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“…In some cases, specific target gene transactivation for each MRF has been reported (Ridgeway et al, 2000;Charbonnier et al, 2002Charbonnier et al, , 2003Suelves et al, 2004). Ectopic expression of Myf5, Myod (Ludolph et al, 1994), or Mrf4 (Chanoine and Hardy, 2003) indicates that MRFs are able to induce ectopic myogenesis in Xenopus. We performed gain of function experiments by injection of synthetic mRNA coding for each MRF at a relative low dose (100 pg per embryo) into a single blastomere at the two-cell stage.…”

Section: Ability Of Myf5 To Induce Ectopic Muscle Structural Gene Expmentioning

confidence: 99%

Myogenic waves and myogenic programs during Xenopus embryonic myogenesis

Gaspera

Armand

Sequeira

et al. 2012

Developmental Dynamics

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Background: Although Xenopus is a key model organism in developmental biology, little is known about the myotome formation in this species. Here, we assessed the expression of myogenic regulatory factors of the Myod family (MRFs) during embryonic development and revealed distinct MRF programs. Results: The expression pattern of each MRF during embryonic development highlights three successive myogenic waves. We showed that a first median and lateral myogenesis initiates before dermomyotome formation: the median cell population expresses Myf5, Myod, and Mrf4, whereas the lateral one expresses Myod, moderate levels of Myogenin and Mrf4. The second wave of myoblasts arising from the dermomyotome is characterized by the full MRF program expression, with high levels of Myogenin. The third wave is revealed by Myf5 expression in the myotome and could contribute to the formation of plurinucleated fibers at larval stages. Furthermore, Myf5-or Myod-expressing anlagen are identified in craniofacial myogenesis. Conclusions: The first median and lateral myogenesis and their associated MRF programs have probably disappeared in mammals. However, some aspects of Xenopus myogenesis have been conserved such as the development of somitic muscles by successive myogenic waves and the existence of Myf5-dependent andindependent lineages. Developmental Dynamics 241:995-1007,

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Overexpression of XMyoD or XMyf5 in Xenopus Embryos Induces the Formation of Enlarged Myotomes through Recruitment of Cells of Nonsomitic Lineage

Cited by 32 publications

References 0 publications

A single cdk inhibitor, p27Xic1, functions beyond cell cycle regulation to promote muscle differentiation inXenopus

A single cdk inhibitor, p27Xic1, functions beyond cell cycle regulation to promote muscle differentiation inXenopus

Myogenic regulatory factors: Redundant or specific functions? Lessons from Xenopus

Myogenic waves and myogenic programs during Xenopus embryonic myogenesis

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