Overexpression of wheat lipid transfer protein gene TaLTP5 increases resistances to Cochliobolus sativus and Fusarium graminearum in transgenic wheat

Zhu, Xinna; Zhao, Li; Xu, Huijun; Zhou, Mengya; Du, Liangcheng; Zhang, Zengyan

doi:10.1007/s10142-012-0286-z

Cited by 65 publications

(32 citation statements)

References 26 publications

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“…Defence-related genes play vital roles in defence against pathogens in plants (Guilleroux and Osbourn, 2004; Seo and Park, 2010; Zhang et al ., 2012). For example, transgenic wheat plants overexpressing a wheat LTP gene or a barley chitinase gene displayed increased resistance to fungal pathogens (Shin et al ., 2008; Zhu et al ., 2012). Although no natural highly resistant wheat cultivar has been identified, the transcription levels of some wheat defence-responsive genes were up-regulated during compatible interactions between wheat roots and Ggt using suppression subtractive hybridization and expressed sequence tag (EST) analysis (Guilleroux and Osbourn, 2004).…”

Section: Discussionmentioning

confidence: 99%

Transgenic wheat expressing Thinopyrum intermedium MYB transcription factor TiMYB2R-1 shows enhanced resistance to the take-all disease

Liu

Yang

Zhou

et al. 2013

Journal of Experimental Botany

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The disease take-all, caused by the fungus Gaeumannomyces graminis, is one of the most destructive root diseases of wheat worldwide. Breeding resistant cultivars is an effective way to protect wheat from take-all. However, little progress has been made in improving the disease resistance level in commercial wheat cultivars. MYB transcription factors play important roles in plant responses to environmental stresses. In this study, an R2R3-MYB gene in Thinopyrum intermedium, TiMYB2R-1, was cloned and characterized. The gene sequence includes two exons and an intron. The expression of TiMYB2R-1 was significantly induced following G. graminis infection. An in vitro DNA binding assay proved that TiMYB2R-1 protein could bind to the MYB-binding site cis-element ACI. Subcellular localization assays revealed that TiMYB2R-1 was localized in the nucleus. TiMYB2R-1 transgenic wheat plants were generated, characterized molecularly, and evaluated for take-all resistance. PCR and Southern blot analyses confirmed that TiMYB2R-1 was integrated into the genomes of three independent transgenic wheat lines by distinct patterns and the transgene was heritable. Reverse transcription–PCR and western blot analyses revealed that TiMYB2R-1 was highly expressed in the transgenic wheat lines. Based on disease response assessments for three successive generations, the significantly enhanced resistance to take-all was observed in the three TiMYB2R-1-overexpressing transgenic wheat lines. Furthermore, the transcript levels of at least six wheat defence-related genes were significantly elevated in the TiMYB2R-1 transgenic wheat lines. These results suggest that engineering and overexpression of TiMYB2R-1 may be used for improving take-all resistance of wheat and other cereal crops.

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Section: Discussionmentioning

confidence: 99%

Transgenic wheat expressing Thinopyrum intermedium MYB transcription factor TiMYB2R-1 shows enhanced resistance to the take-all disease

Liu

Yang

Zhou

et al. 2013

Journal of Experimental Botany

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“…Several possible mechanisms of action for this LTP have been proposed based on the previously known roles of LTPs in plant disease resistance. Although, no functional data are available for this LTP, the over-expression of TaLTP5, another LTP, in wheat confers increased FHB resistance (Zhu et al, 2012).…”

Section: Qfhsifa-5amentioning

confidence: 99%

Transcriptomics of cereal–Fusarium graminearum interactions: what we have learned so far

Kazan

Gardiner

2017

Molecular Plant Pathology

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The ascomycete fungal pathogen Fusarium graminearum causes the globally important Fusarium head blight (FHB) disease on cereal hosts, such as wheat and barley. In addition to reducing grain yield, infection by this pathogen causes major quality losses. In particular, the contamination of food and feed with the F. graminearum trichothecene toxin deoxynivalenol (DON) can have many adverse short- and long-term effects on human and animal health. During the last decade, the interaction between F. graminearum and both cereal and model hosts has been extensively studied through transcriptomic analyses. In this review, we present an overview of how such analyses have advanced our understanding of this economically important plant-microbe interaction. From a host point of view, the transcriptomes of FHB-resistant and FHB-susceptible cereal genotypes, including near-isogenic lines (NILs) that differ by the presence or absence of quantitative trait loci (QTLs), have been studied to understand the mechanisms of disease resistance afforded by such QTLs. Transcriptomic analyses employed to dissect host responses to DON have facilitated the identification of the genes involved in toxin detoxification and disease resistance. From the pathogen point of view, the transcriptome of F. graminearum during pathogenic vs. saprophytic growth, or when infecting different cereal hosts or different tissues of the same host, have been studied. In addition, comparative transcriptomic analyses of F. graminearum knock-out mutants with altered virulence have provided new insights into pathogenicity-related processes. The F. graminearum transcriptomic data generated over the years are now being exploited to build a systems level understanding of the biology of this pathogen, with an ultimate aim of developing effective and sustainable disease prevention strategies.

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“…Therefore, biotechnological solutions to durable FHB resistance might be an attractive alternative. So far, transgenic approaches in wheat for FHB resistance mainly focused on the constitutive overexpression of plant genes encoding candidate proteins for defense signaling or cell wall-related defense responses (Makandar et al , 2006; Shin et al , 2008; Volpi et al , 2011; Xiang et al , 2011; Ferrari et al , 2012; Zhu et al , 2012). Such overexpression approaches, despite having resulted in partial protection, bear the risk of being energy demanding or having unwanted side effects on growth of non-infected plants (Gurr and Rushton, 2005; Goto et al , 2016).…”

Section: Introductionmentioning

confidence: 99%

Host-induced silencing ofFusarium culmorumgenes protects wheat from infection

Chen

Kastner

Nowara

et al. 2016

EXBOTJ

124

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Overexpression of wheat lipid transfer protein gene TaLTP5 increases resistances to Cochliobolus sativus and Fusarium graminearum in transgenic wheat

Cited by 65 publications

References 26 publications

Transgenic wheat expressing Thinopyrum intermedium MYB transcription factor TiMYB2R-1 shows enhanced resistance to the take-all disease

Transgenic wheat expressing Thinopyrum intermedium MYB transcription factor TiMYB2R-1 shows enhanced resistance to the take-all disease

Transcriptomics of cereal–Fusarium graminearum interactions: what we have learned so far

Host-induced silencing ofFusarium culmorumgenes protects wheat from infection

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